PMID- 37076464
OWN - NLM
STAT- MEDLINE
DCOM- 20230831
LR  - 20230831
IS  - 1875-5402 (Electronic)
IS  - 1386-2073 (Linking)
VI  - 26
IP  - 14
DP  - 2023
TI  - Nomilin Attenuates Lipopolysaccharide-Induced Inflammatory Response by Binding 
      with Myeloid Differentiation Protein-2.
PG  - 2469-2475
LID - 10.2174/1386207326666230418112827 [doi]
AB  - BACKGROUND: Nomilin shows anti-inflammatory activity by inhibiting the activation 
      of the Toll-like receptor 4 (TLR 4)/NF-kappaB pathway. However, the key target of the 
      anti-inflammatory activity of nomilin has not been elaborated and needs further 
      exploration. OBJECTIVE: This study aimed to assess the drug potential of nomilin 
      and its ability to target myeloid differentiation protein 2 (MD-2) as a mechanism 
      underlying the anti-inflammatory activity of nomilin on the lipopolysaccharide 
      (LPS)-TLR4/MD-2-NF-kappaB signaling pathways. METHODS: The methods of ForteBio and 
      molecular docking were used to investigate the internation between MD-2 and 
      nomilin. 3-(4,5)-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide 
      experiment was performed to test the effect of nomilin on cell viability. 
      Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, and 
      Western blot experiments were carried out to assess the anti-inflammatory 
      activity and possible mechanism of nomilin in vitro. RESULTS: The results 
      indicated that nomilin exhibited binding affinity with MD-2. Nomilin 
      significantly reduced the release and expression of NO, IL-6, TNF-alpha, and IL-1beta 
      induced by LPS in vitro. It inhibited the expression of LPS-TLR4/MD-2-NF-kappaB 
      signaling pathway proteins, such as TLR4, Myd88, P65, P-P65, and iNOS. 
      CONCLUSION: Our results suggested that nomilin had therapeutic potential and was 
      bound to MD-2. Nomilin exhibited anti-inflammatory activity by binding to the key 
      protein MD-2 and inhibiting the LPS-TLR4/MD-2-NF-kappaB signaling pathway.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Chen, Yuting
AU  - Chen Y
AD  - College of Pharmacy, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, 110847, China.
FAU - Guo, Song
AU  - Guo S
AD  - Department of Computer Application, Shenyang Sport University, Shenyang, 110102, 
      China.
FAU - Chen, Guirong
AU  - Chen G
AD  - College of Pharmacy, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, 110847, China.
AD  - Department of Pharmacy, 967th Hospital of the Joint Logistics Support Force of 
      the Chinese People's Liberation Army, Dalian, Liaoning, China.
FAU - Liu, Chang
AU  - Liu C
AD  - College of Pharmacy, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, 110847, China.
FAU - Zhang, Mingbo
AU  - Zhang M
AD  - College of Pharmacy, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, 110847, China.
FAU - Wang, Xiaobo
AU  - Wang X
AD  - Department of Pharmacy, 967th Hospital of the Joint Logistics Support Force of 
      the Chinese People's Liberation Army, Dalian, Liaoning, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Comb Chem High Throughput Screen
JT  - Combinatorial chemistry & high throughput screening
JID - 9810948
RN  - 0 (NF-kappa B)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptor 4)
RN  - DRM0753K4T (nomilin)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - *NF-kappa B/metabolism
MH  - *Lipopolysaccharides/pharmacology
MH  - Toll-Like Receptor 4/metabolism
MH  - Molecular Docking Simulation
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cell Differentiation
OTO - NOTNLM
OT  - Anti-inflammatory activity
OT  - ELISA
OT  - LPS-TLR4/MD-2-NF-kappaB signaling pathway
OT  - lipopolysaccharide
OT  - myeloid differentiation protein 2
OT  - nomilin
EDAT- 2023/04/20 00:41
MHDA- 2023/08/31 06:42
CRDT- 2023/04/19 23:13
PHST- 2022/07/21 00:00 [received]
PHST- 2023/02/12 00:00 [revised]
PHST- 2023/03/03 00:00 [accepted]
PHST- 2023/08/31 06:42 [medline]
PHST- 2023/04/20 00:41 [pubmed]
PHST- 2023/04/19 23:13 [entrez]
AID - CCHTS-EPUB-131082 [pii]
AID - 10.2174/1386207326666230418112827 [doi]
PST - ppublish
SO  - Comb Chem High Throughput Screen. 2023;26(14):2469-2475. doi: 
      10.2174/1386207326666230418112827.