PMID- 37078133
OWN - NLM
STAT- MEDLINE
DCOM- 20230918
LR  - 20230920
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 43
IP  - 10
DP  - 2023 Oct
TI  - Development of a yeast reporter gene assay to detect ligands of freshwater 
      cladoceran Daphnia magna ultraspiracle, a homolog of vertebrate retinoid X 
      receptors.
PG  - 1447-1461
LID - 10.1002/jat.4476 [doi]
AB  - Endocrine-disrupting chemicals (EDCs) often affect homeostatic regulation in 
      living organisms by directly acting on nuclear receptors (NRs). Retinoid X 
      receptors (RXRs), the most highly conserved members of the NR superfamily during 
      evolution, function as partners to form heterodimers with other NRs, such as 
      retinoic acid, thyroid hormone, and vitamin D(3) receptors. RXRs also 
      homodimerize and induce the expression of target genes upon binding with their 
      natural ligand, 9-cis-retinoic acid (9cRA), and typical EDCs organotin compounds, 
      such as tributyltin and triphenyltin. In the present study, we established a new 
      yeast reporter gene assay (RGA) to detect the ligands of freshwater cladoceran 
      Daphnia magna ultraspiracle (Dapma-USP), a homolog of vertebrate RXRs. D. magna 
      has been used as a representative crustacean species for aquatic EDC assessments 
      in the Organization for Economic Corporation and Development test guidelines. 
      Dapma-USP was expressed along with the Drosophila melanogaster steroid receptor 
      coactivator Taiman in yeast cells carrying the lacZ reporter plasmid. The RGA for 
      detecting agonist activity of organotins and o-butylphenol was improved by use of 
      mutant yeast strains lacking genes encoding cell wall mannoproteins and/or plasma 
      membrane drug efflux pumps as hosts. We also showed that a number of other human 
      RXR ligands, phenol and bisphenol A derivatives, and terpenoid compounds such as 
      9c-RA exhibited antagonist activity on Dapma-USP. Our newly established 
      yeast-based RGA system is valuable as the first screening tool to detect ligand 
      substances for Dapma-USP and for evaluating the evolutionary divergence of the 
      ligand responses of RXR homologs between humans and D. magna.
CI  - (c) 2023 John Wiley & Sons Ltd.
FAU - Ito-Harashima, Sayoko
AU  - Ito-Harashima S
AD  - Department of Biological Science, Graduate School of Science, Osaka Prefecture 
      University, Sakai, Japan.
AD  - Department of Applied Biological Chemistry, Graduate School of Agriculture, Osaka 
      Metropolitan University, Sakai, Japan.
FAU - Tsubouchi, Yumiko
AU  - Tsubouchi Y
AD  - Department of Biological Science, Graduate School of Science, Osaka Prefecture 
      University, Sakai, Japan.
FAU - Takada, Eiji
AU  - Takada E
AD  - Department of Biological Science, Graduate School of Science, Osaka Prefecture 
      University, Sakai, Japan.
FAU - Kawanishi, Masanobu
AU  - Kawanishi M
AD  - Department of Biological Science, Graduate School of Science, Osaka Prefecture 
      University, Sakai, Japan.
AD  - Department of Biological Chemistry, Graduate School of Science, Osaka 
      Metropolitan University, Sakai, Japan.
FAU - Yagi, Takashi
AU  - Yagi T
AUID- ORCID: 0000-0002-5476-8424
AD  - Department of Biological Science, Graduate School of Science, Osaka Prefecture 
      University, Sakai, Japan.
AD  - Department of Biological Chemistry, Graduate School of Science, Osaka 
      Metropolitan University, Sakai, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230504
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Ligands)
RN  - 53282-60-3 (4-dansyl aminophenylmercuric acetate)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Retinoid X Receptors/genetics/metabolism
MH  - Ligands
MH  - *Saccharomyces cerevisiae/genetics/metabolism
MH  - Genes, Reporter
MH  - *Daphnia/genetics/metabolism
MH  - Drosophila melanogaster/genetics
MH  - Vertebrates/genetics/metabolism
OTO - NOTNLM
OT  - Daphnia magna
OT  - organotin
OT  - retinoid X receptor
OT  - ultraspiracle
OT  - yeast reporter gene assay
EDAT- 2023/04/20 06:42
MHDA- 2023/09/18 12:44
CRDT- 2023/04/20 03:03
PHST- 2023/04/16 00:00 [revised]
PHST- 2022/10/01 00:00 [received]
PHST- 2023/04/17 00:00 [accepted]
PHST- 2023/09/18 12:44 [medline]
PHST- 2023/04/20 06:42 [pubmed]
PHST- 2023/04/20 03:03 [entrez]
AID - 10.1002/jat.4476 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2023 Oct;43(10):1447-1461. doi: 10.1002/jat.4476. Epub 2023 May 
      4.