PMID- 37078361
OWN - NLM
STAT- MEDLINE
DCOM- 20230614
LR  - 20230614
IS  - 1696-3547 (Electronic)
IS  - 0214-6282 (Linking)
VI  - 67
IP  - 1
DP  - 2023
TI  - Circ-JA760602 promotes the apoptosis of hypoxia-induced cardiomyocytes by 
      transcriptionally suppressing BCL2.
PG  - 9-17
LID - 10.1387/ijdb.220150jl [doi]
AB  - Acute myocardial infarction (AMI) is myocardial necrosis caused by the complete 
      or partial obstruction of a coronary artery. Circular RNAs (circRNAs) have been 
      proven as regulators in the progression of various human diseases, including AMI. 
      However, the role of novel circ-JA760602 in AMI remains unknown. Here, we 
      investigated the role of circ-JA760602 in modulating the apoptosis of 
      hypoxia-induced AMI cells using the AC16 cardiomyocyte in vitro cell model. The 
      expression of circ-JA760602 in AC16 cardiomyocytes subjected to hypoxia was 
      measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell 
      viability was measured by cell counting kit-8 (CCK-8) assay. Apoptosis of 
      cardiomyocytes was evaluated by TUNEL assay and flow cytometry analysis. The 
      cellular location of circ-JA760602 was identified through fluorescence in situ 
      hybridization (FISH) assay and subcellular fractionation assay. The downstream 
      molecular mechanisms of circ-JA760602 were demonstrated by luciferase reporter 
      assay, RNA binding protein immunoprecipitation (RIP) assay and chromatin 
      immunoprecipitation (ChIP) assay. Rescue assays were performed to demonstrate the 
      effects of BCL2 knockdown on circ-JA760602 silencing-mediated cardiomyocyte 
      apoptosis. Circ-JA760602 expression was elevated after hypoxia treatment. 
      Knockdown of circ-JA760602 enhanced viability and curbed apoptosis of 
      hypoxia-treated cardiomyocytes. EGR1 and E2F1 could activate BCL2 transcription. 
      Cytoplasmic circ-JA760602 bound with EGR1 and E2F1 to thus inhibit their nuclear 
      translocation. BCL2 knockdown reversed the effects of circ-JA760602 silencing on 
      the apoptosis of hypoxia-treated AC16 cells. Circ-JA760602 promotes 
      hypoxia-induced apoptosis of cardiomyocytes by binding with EGR1 and E2F1 to 
      inhibit the transcriptional activation of BCL2.
FAU - Li, Chao
AU  - Li C
AD  - Department of Cardiology, the First Affiliated Hospital of Anhui Medical 
      University; the Fifth Clinical College, Medical University of Anhui; Hefei 
      Hospital Affiliated to Medical University of Anhui (the Second People's Hospital 
      of Hefei), Hefei, Anhui, China.
FAU - Wang, Jingwen
AU  - Wang J
AD  - Department of Cardiology, the Second Hospital Affiliated to Medical University of 
      Anhui, Hefei, Anhui Province, China.
FAU - Feng, Jun
AU  - Feng J
AD  - Department of Cardiology, Hefei Hospital Affiliated to Medical University of 
      Anhui (the Second People's Hospital of Hefei), Hefei, Anhui Province, China.
FAU - Zhou, Gaoliang
AU  - Zhou G
AD  - Department of Cardiology, Hefei Hospital Affiliated to Medical University of 
      Anhui (the Second People's Hospital of Hefei), Hefei, Anhui Province, China.
FAU - Jiang, Yongjin
AU  - Jiang Y
AD  - Department of Cardiology, Hefei Hospital Affiliated to Medical University of 
      Anhui (the Second People's Hospital of Hefei), Hefei, Anhui Province, China.
FAU - Luo, Chunmiao
AU  - Luo C
AD  - Department of Cardiology, Hefei Hospital Affiliated to Medical University of 
      Anhui (the Second People's Hospital of Hefei), Hefei, Anhui Province, China.
FAU - Cheng, Ziping
AU  - Cheng Z
AD  - Department of Cardiology, the First Hospital Affiliated to Medical University of 
      Anhui, Hefei, Anhui Province, China.
FAU - Li, Jiehua
AU  - Li J
AD  - Department of Cardiology, the First Hospital Affiliated to Medical University of 
      Anhui, Hefei, Anhui Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
RN  - 0 (BCL2 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Humans
MH  - Apoptosis/genetics
MH  - Cell Proliferation
MH  - Hypoxia
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs
MH  - *Myocytes, Cardiac
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - RNA, Circular
EDAT- 2023/04/20 06:41
MHDA- 2023/06/06 06:42
CRDT- 2023/04/20 05:14
PHST- 2023/06/06 06:42 [medline]
PHST- 2023/04/20 06:41 [pubmed]
PHST- 2023/04/20 05:14 [entrez]
AID - 220150jl [pii]
AID - 10.1387/ijdb.220150jl [doi]
PST - ppublish
SO  - Int J Dev Biol. 2023;67(1):9-17. doi: 10.1387/ijdb.220150jl.