PMID- 37079130
OWN - NLM
STAT- MEDLINE
DCOM- 20230505
LR  - 20230505
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 46
IP  - 6
DP  - 2023 Jun
TI  - Metabolic analysis of Schizochytrium sp. mutants with high EPA content achieved 
      with ARTP mutagenesis screening.
PG  - 893-901
LID - 10.1007/s00449-023-02874-5 [doi]
AB  - Eicosapentaenoic acid (EPA) belonged to the omega-3 series of polyunsaturated fatty 
      acids and had physiological functions lipid as regulating blood lipid and 
      preventing cardiovascular diseases. Schizochytrium sp. was considered to be a 
      potential industrial fermentation strain of EPA because of its fast growth, high 
      oil content, and simple fatty acid composition. However, Schizochytrium sp. 
      produced EPA with low production efficiency and a long synthesis path. This 
      research aims to improve the yield of EPA in Schizochytrium sp. by ARTP 
      mutagenesis and to reveal the mechanism of high-yield EPA through transcriptome 
      analysis. ARTP mutagenesis screening yielded the mutant M12 that whereas the 
      productivity of EPA increased 108% reaching 0.48 g/L, the total fatty acid 
      concentration was 13.82 g/L with an increase of 13.7%. The transcriptomics 
      revealed 2995 differentially expressed genes were identified between M12 and the 
      wild-type strain and transcripts involved in carbohydrate, amino acid, energy, 
      and lipid metabolism were up-regulated. Among them, the hexokinase (HK) and the 
      phosphofructokinase genes (PFK), which can catalyze pyruvate to acetyl-CoA, were 
      increased 2.23-fold and 1.78-fold. Glucose-6-phosphate dehydrogenase (G6PD) and 
      glutamate dehydrogenase (GLDH), which can both generate NADPH, were increased by 
      1.67-fold and 3.11-fold. Furthermore, in the EPA synthesis module, the expression 
      of 3-oxoacyl-[acyl-carrier protein] reductase(fabG) and carbonyl reductase 4 / 
      3-oxoacyl-[acyl-carrier protein] reductase beta subunit(CBR4), also up-regulated 
      1.11-fold and 2.67-fold. These may lead to increases in cell growth. The results 
      provide an important reference for further research on promoting fatty acid and 
      EPA accumulation in Schizochytrium sp.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Wei, Xinyu
AU  - Wei X
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Wang, Yuzhou
AU  - Wang Y
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Liu, Xiner
AU  - Liu X
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Hu, Zijian
AU  - Hu Z
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Qian, Jinyi
AU  - Qian J
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Shi, Tianqiong
AU  - Shi T
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Wang, Yuetong
AU  - Wang Y
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China.
FAU - Ye, Chao
AU  - Ye C
AD  - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 
      Nanjing, 210023, People's Republic of China. chaoye09@njnu.edu.cn.
LA  - eng
GR  - 22208167/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230420
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 0 (Fatty Acids)
RN  - EC 1.- (Oxidoreductases)
RN  - 0 (Carrier Proteins)
SB  - IM
MH  - *Eicosapentaenoic Acid/metabolism
MH  - Docosahexaenoic Acids/metabolism
MH  - *Stramenopiles
MH  - Fatty Acids/metabolism
MH  - Mutagenesis
MH  - Oxidoreductases/metabolism
MH  - Carrier Proteins/genetics
OTO - NOTNLM
OT  - ARTP
OT  - EPA
OT  - Schizochytrium sp.
OT  - Transcriptome
EDAT- 2023/04/20 13:41
MHDA- 2023/05/05 06:42
CRDT- 2023/04/20 11:17
PHST- 2023/01/17 00:00 [received]
PHST- 2023/04/10 00:00 [accepted]
PHST- 2023/05/05 06:42 [medline]
PHST- 2023/04/20 13:41 [pubmed]
PHST- 2023/04/20 11:17 [entrez]
AID - 10.1007/s00449-023-02874-5 [pii]
AID - 10.1007/s00449-023-02874-5 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2023 Jun;46(6):893-901. doi: 10.1007/s00449-023-02874-5. 
      Epub 2023 Apr 20.