PMID- 37079165
OWN - NLM
STAT- MEDLINE
DCOM- 20230706
LR  - 20230706
IS  - 1613-2246 (Electronic)
IS  - 0021-5155 (Linking)
VI  - 67
IP  - 4
DP  - 2023 Jul
TI  - Galectin-3, a damage-associated molecular pattern, in tears of patients with 
      vernal keratoconjunctivitis.
PG  - 431-439
LID - 10.1007/s10384-023-00994-9 [doi]
AB  - PURPOSE: Galectin-3 is a damage-associated molecular pattern (DAMPs), released 
      from damaged or dying cells. In this study, we investigated the concentration and 
      source of galectin-3 in the tears of patients with vernal keratoconjunctivitis 
      (VKC) and evaluated whether the concentration of galectin-3 in tears represents a 
      biomarker of corneal epithelial damage. STUDY DESIGN: Clinical and experimental. 
      METHODS: We measured the concentration of galectin-3 in tear samples from 26 
      patients with VKC and 6 healthy controls by enzyme-linked immunosorbent assay 
      (ELISA). The expression of galectin-3 in cultured human corneal epithelial cells 
      (HCEs) stimulated with or without tryptase or chymase was investigated by 
      polymerase chain reaction (PCR), ELISA, and Western blotting. We also estimated 
      the concentration of galectin-3 in the supernatants of cultured HCEs induced to 
      necrosis. Finally, we investigated whether recombinant galectin-3 induced the 
      expression of various genes related to cell migration or the cell cycle in HCEs 
      by using microarray analysis. RESULTS: High concentrations of galectin-3 were 
      detected in the tears of patients with VKC. The concentration showed significant 
      correlation with the severity of corneal epithelial damage. Stimulation of 
      cultured HCEs with various concentrations of tryptase or chymase had no effect on 
      the expression of galectin-3. However, high concentrations of galectin-3 were 
      detected in the supernatants of necrotic HCEs. Recombinant human galectin-3 
      induced various cell migration- and cell cycle-related genes. CONCLUSION: The 
      concentrations of galectin-3 in the tears of patients with VKC may represent a 
      biomarker of the severity of corneal epithelial damage.
CI  - (c) 2023. Japanese Ophthalmological Society.
FAU - Ito, Yousuke
AU  - Ito Y
AD  - Juntendo University Graduate School of Medicine, Tokyo, Japan.
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, 2-1-1, 
      Tomioka, Urayasu-shi, Chiba, Japan.
FAU - Usui-Ouchi, Ayumi
AU  - Usui-Ouchi A
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, 2-1-1, 
      Tomioka, Urayasu-shi, Chiba, Japan.
FAU - Ebihara, Nobuyuki
AU  - Ebihara N
AD  - Department of Ophthalmology, Juntendo University Urayasu Hospital, 2-1-1, 
      Tomioka, Urayasu-shi, Chiba, Japan. ebihara@juntendo.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20230420
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
RN  - EC 3.4.21.39 (Chymases)
RN  - 0 (Galectin 3)
RN  - EC 3.4.21.59 (Tryptases)
RN  - 0 (LGALS3 protein, human)
SB  - IM
MH  - Humans
MH  - Chymases/metabolism
MH  - *Conjunctivitis, Allergic/diagnosis/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Galectin 3/genetics/metabolism
MH  - Tears/metabolism
MH  - Tryptases/metabolism
OTO - NOTNLM
OT  - Chymase
OT  - DAMPs (damage-associated molecular patterns)
OT  - Galectin-3
OT  - Tryptase
OT  - Vernal keratoconjunctivitis
EDAT- 2023/04/20 13:41
MHDA- 2023/07/03 06:41
CRDT- 2023/04/20 11:18
PHST- 2022/09/08 00:00 [received]
PHST- 2023/02/24 00:00 [accepted]
PHST- 2023/07/03 06:41 [medline]
PHST- 2023/04/20 13:41 [pubmed]
PHST- 2023/04/20 11:18 [entrez]
AID - 10.1007/s10384-023-00994-9 [pii]
AID - 10.1007/s10384-023-00994-9 [doi]
PST - ppublish
SO  - Jpn J Ophthalmol. 2023 Jul;67(4):431-439. doi: 10.1007/s10384-023-00994-9. Epub 
      2023 Apr 20.