PMID- 37080405
OWN - NLM
STAT- MEDLINE
DCOM- 20230612
LR  - 20230612
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 241
DP  - 2023 Jun 30
TI  - Carboxymethylcellulose hydrogels crosslinked with keratin nanoparticles for 
      efficient prednisolone delivery.
PG  - 124497
LID - S0141-8130(23)01391-0 [pii]
LID - 10.1016/j.ijbiomac.2023.124497 [doi]
AB  - Carboxymethylcellulose (CMC) and keratin nanoparticle (KNP) hydrogels were 
      obtained, characterized, and applied as drug delivery systems (DDSs) for the 
      first time. Lyophilized CMC/KNP mixtures containing 10, 25, and 50 wt% of KNPs 
      were kept at 170  degrees C for 90 min to crosslink CMC chains through a solid-state 
      reaction with the KNPs. The hydrogels were characterized by infrared 
      spectroscopy, thermal analyses, X-ray diffraction, mechanical measurements, and 
      scanning electron microscopy. The infrared spectra indicated the formation of 
      ester and amide linkages between crosslinked CMC and KNPs. The elastic modulus of 
      the hydrogel containing 10 wt% KNPs was 2-fold higher than that of the hydrogel 
      containing 50 wt% KNPs. The mechanical properties influenced the hydrogel 
      stability and water uptake. The anti-inflammatory prednisolone (PRED) drug was 
      incorporated into the hydrogels, and the release mechanism was investigated. The 
      hydrogels supported PRED release by drug desorption for approximately 360 h. A 
      sustained release mechanism was achieved. The CMC/KNP and CMC/KNP/PRED hydrogels 
      were cytocompatible toward mammalian cells. The CMC/KNP/PRED set imparted the 
      highest cell viability after 7 days of incubation. This study showed a 
      straightforward procedure to create DDSs (chemically crosslinked) based on 
      polysaccharides and proteins for efficient PRED delivery.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Silva, Otavio A
AU  - Silva OA
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil.
FAU - Pella, Michelly G
AU  - Pella MG
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil.
FAU - Sabino, Roberta M
AU  - Sabino RM
AD  - School of Advanced Materials Discovery, Colorado State University (CSU), Fort 
      Collins, CO, USA; Institute for Medical Engineering and Science, Massachusetts 
      Institute of Technology (MIT), Cambridge, MA, USA.
FAU - Popat, Ketul C
AU  - Popat KC
AD  - School of Advanced Materials Discovery, Colorado State University (CSU), Fort 
      Collins, CO, USA; Department of Mechanical Engineering, Colorado State University 
      (CSU), Fort Collins, CO, USA.
FAU - Kipper, Matt J
AU  - Kipper MJ
AD  - School of Advanced Materials Discovery, Colorado State University (CSU), Fort 
      Collins, CO, USA; Department of Chemical and Biological Engineering, Colorado 
      State University (CSU), Fort Collins, CO, USA.
FAU - Rubira, Adley F
AU  - Rubira AF
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil.
FAU - Follmann, Heveline D M
AU  - Follmann HDM
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil.
FAU - Silva, Rafael
AU  - Silva R
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil.
FAU - Martins, Alessandro F
AU  - Martins AF
AD  - Group of Polymers and Composite Materials, Department of Chemistry, State 
      University of Maringa (UEM), Maringa, PR, Brazil; Laboratory of Materials, 
      Macromolecules, and Composites, Federal University of Technology-Parana (UTFPR), 
      Apucarana, PR, Brazil; Department of Chemical and Biological Engineering, 
      Colorado State University (CSU), Fort Collins, CO, USA. Electronic address: 
      afmartins@utfpr.edu.br.
LA  - eng
PT  - Journal Article
DEP - 20230418
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Hydrogels)
RN  - 68238-35-7 (Keratins)
RN  - K679OBS311 (Carboxymethylcellulose Sodium)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Animals
MH  - *Hydrogels/chemistry
MH  - Keratins
MH  - Carboxymethylcellulose Sodium/chemistry
MH  - Prednisolone/pharmacology
MH  - Anti-Inflammatory Agents
MH  - *Nanoparticles
MH  - Mammals
OTO - NOTNLM
OT  - Anti-inflammatory
OT  - Polysaccharides
OT  - Release
COIS- Declaration of competing interest The authors have no competing financial 
      interests to declare or personal relationships that can influence the work 
      reported in this paper.
EDAT- 2023/04/21 00:42
MHDA- 2023/06/12 06:42
CRDT- 2023/04/20 19:26
PHST- 2023/02/07 00:00 [received]
PHST- 2023/04/04 00:00 [revised]
PHST- 2023/04/13 00:00 [accepted]
PHST- 2023/06/12 06:42 [medline]
PHST- 2023/04/21 00:42 [pubmed]
PHST- 2023/04/20 19:26 [entrez]
AID - S0141-8130(23)01391-0 [pii]
AID - 10.1016/j.ijbiomac.2023.124497 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Jun 30;241:124497. doi: 10.1016/j.ijbiomac.2023.124497. 
      Epub 2023 Apr 18.