PMID- 37080513
OWN - NLM
STAT- MEDLINE
DCOM- 20230509
LR  - 20230509
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 328
DP  - 2023 Jul 1
TI  - Study of DNA methylation of hsd17beta, er and reproductive endocrine disrupting 
      effects in female Chlamys farreri under benzo[a]pyrene stress.
PG  - 121667
LID - S0269-7491(23)00669-3 [pii]
LID - 10.1016/j.envpol.2023.121667 [doi]
AB  - Benzo[a]pyrene (B[a]P) is one kind of persistent organic pollutants (POPs) in the 
      marine environment which has multiple toxic effects. However, epigenetic studies 
      correlated with reproductive endocrine disruption in invertebrates have not been 
      explored. In our study, Chlamys farreri in the mature stage were exposed to B[a]P 
      (0, 0.4, 2 and 10 mug/L) for 5 and 10 d to explore the effects on reproductive 
      endocrine and DNA methylation. The results proved that B[a]P stress significantly 
      restrained the growth of mature oocytes, reduced the content of sex hormones, and 
      affected the expression of genes related to ovarian development. Histological 
      observation showed that the ovarian microstructure was damaged. The detection of 
      SAM/SAH, dnmts, GNMT in the ovary showed that the level of global DNA methylation 
      fluctuated. Significant hypermethylation of the hsd17beta promoter region in the 
      ovary was associated with a significant downregulation of its gene expression. In 
      summary, our results suggested that exposure to B[a]P might affect DNA 
      methylation to regulate key reproductive genes, interfere with the synthesis of 
      sex hormones, and inhibit ovarian development. These findings provide a basis for 
      a better understanding of how epigenetic mechanisms are involved in the response 
      of marine invertebrates to POPs stress, opening up new avenues for incorporating 
      environmental epigenetic approaches into marine invertebrate management and 
      conservation plans.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Tang, Jian
AU  - Tang J
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China.
FAU - Song, Aimin
AU  - Song A
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China.
FAU - Pan, Luqing
AU  - Pan L
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China. Electronic address: panlq@ouc.edu.cn.
FAU - Miao, Jingjing
AU  - Miao J
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China.
FAU - Li, Zeyuan
AU  - Li Z
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China.
FAU - Zhou, Yueyao
AU  - Zhou Y
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao, 266003, China.
LA  - eng
PT  - Journal Article
DEP - 20230418
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 0 (Gonadal Steroid Hormones)
SB  - IM
MH  - Female
MH  - Animals
MH  - *Benzo(a)pyrene/toxicity/metabolism
MH  - DNA Methylation
MH  - Ovary/metabolism
MH  - Gonadal Steroid Hormones/metabolism
MH  - *Pectinidae/genetics
OTO - NOTNLM
OT  - Benzo[a]pyrene
OT  - Chlamys farreri
OT  - DNA methylation
OT  - Ovarian development
OT  - Reproductive toxicity
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/04/21 00:41
MHDA- 2023/05/09 10:16
CRDT- 2023/04/20 19:28
PHST- 2022/11/20 00:00 [received]
PHST- 2023/04/14 00:00 [revised]
PHST- 2023/04/17 00:00 [accepted]
PHST- 2023/05/09 10:16 [medline]
PHST- 2023/04/21 00:41 [pubmed]
PHST- 2023/04/20 19:28 [entrez]
AID - S0269-7491(23)00669-3 [pii]
AID - 10.1016/j.envpol.2023.121667 [doi]
PST - ppublish
SO  - Environ Pollut. 2023 Jul 1;328:121667. doi: 10.1016/j.envpol.2023.121667. Epub 
      2023 Apr 18.