PMID- 37084957
OWN - NLM
STAT- MEDLINE
DCOM- 20230816
LR  - 20230817
IS  - 1872-9428 (Electronic)
IS  - 0166-6851 (Linking)
VI  - 255
DP  - 2023 Sep
TI  - Designing a multi-epitope chimeric protein from different potential targets: A 
      potential vaccine candidate against Plasmodium.
PG  - 111560
LID - S0166-6851(23)00018-X [pii]
LID - 10.1016/j.molbiopara.2023.111560 [doi]
AB  - Malaria is an infectious disease that has been a continuous threat to mankind 
      since the time immemorial. Owing to the complex multi-staged life cycle of the 
      plasmodium parasite, an effective malaria vaccine which is fully protective 
      against the parasite infection is urgently needed to deal with the challenges. In 
      the present study, essential parasite proteins were identified and a chimeric 
      protein with multivalent epitopes was generated. The designed chimeric protein 
      consists of best potential B and T cell epitopes from five different essential 
      parasite proteins. Physiochemical studies of the chimeric protein showed that the 
      modeled vaccine construct was thermo-stable, hydrophilic and antigenic in nature. 
      And the binding of the vaccine construct with Toll-like receptor-4 (TLR-4) as 
      revealed by the molecular docking suggests the possible interaction and role of 
      the vaccine construct in activating the innate immune response. The constructed 
      vaccine being a chimeric protein containing epitopes from different potential 
      candidates could target different stages or pathways of the parasite. Moreover, 
      the approach used in this study is time and cost effective, and can be applied in 
      the discoveries of new potential vaccine targets for other pathogens.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Devi, Sanasam Bijara
AU  - Devi SB
AD  - Department of Life science & Bioinformatics, Assam University, Silchar 788011 
      India. Electronic address: jarasanasam@gmail.com.
FAU - Kumar, Sanjeev
AU  - Kumar S
AD  - Department of Life science & Bioinformatics, Assam University, Silchar 788011 
      India.
LA  - eng
PT  - Journal Article
DEP - 20230420
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Malaria Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Molecular Docking Simulation
MH  - Plasmodium falciparum/genetics
MH  - *Malaria Vaccines/genetics
MH  - Epitopes, T-Lymphocyte/genetics/chemistry
MH  - *Plasmodium
MH  - *Parasites
MH  - Recombinant Fusion Proteins/genetics
MH  - Computational Biology
OTO - NOTNLM
OT  - Chimeric protein
OT  - Malaria
OT  - Peptide vaccine
OT  - Plasmodium falciparum
OT  - Reverse vaccinology
COIS- Declaration of Competing Interest The authors declare no conflicting interest in 
      publishing the article.
EDAT- 2023/04/22 10:42
MHDA- 2023/08/16 06:42
CRDT- 2023/04/21 19:28
PHST- 2022/07/13 00:00 [received]
PHST- 2023/03/30 00:00 [revised]
PHST- 2023/04/03 00:00 [accepted]
PHST- 2023/08/16 06:42 [medline]
PHST- 2023/04/22 10:42 [pubmed]
PHST- 2023/04/21 19:28 [entrez]
AID - S0166-6851(23)00018-X [pii]
AID - 10.1016/j.molbiopara.2023.111560 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 2023 Sep;255:111560. doi: 
      10.1016/j.molbiopara.2023.111560. Epub 2023 Apr 20.