PMID- 37086652
OWN - NLM
STAT- MEDLINE
DCOM- 20230515
LR  - 20230515
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 259
DP  - 2023 Jun
TI  - Thyroid endocrine disruption induced by [C(8)mim]Br: An integrated in vivo, in 
      vitro, and in silico study.
PG  - 106535
LID - S0166-445X(23)00138-8 [pii]
LID - 10.1016/j.aquatox.2023.106535 [doi]
AB  - Conventional thyroid-disrupting chemicals (TDCs) such as polybrominated diphenyl 
      ethers, polychlorinated biphenyls, and bisphenols perturb animal's thyroid 
      endocrine system by mimicking the action of endogenous thyroid hormones (THs), 
      since they share a similar backbone structure of coupled benzene rings with THs. 
      1-methyl-3-octylimidazolium bromide ([C(8)mim]Br), a commonly used ionic liquid 
      (IL), has no structural similarity to THs. Whether it interferes with thyroid 
      function and how its mode of action differs from conventional TDCs is largely 
      unknown. Herein, zebrafish embryo-larvae experiments (in vivo), GH3 cell line 
      studies (in vitro), and molecular simulation analyses (in silico) were carried 
      out to explore the effect of [C(8)mim]Br on thyroid homeostasis and its 
      underlying mechanism. Molecular docking results suggested that [C(8)mim](+) 
      likely bound to retinoid X receptors (RXRs), which may compromise the formation 
      of TH receptor/RXR heterodimers. This then perturbed the negative regulation of 
      thyroid-stimulating hormone beta (tshbeta) transcription by T3 in GH3 cell line. The 
      resulting enhancement of tshbeta expression further caused hyperthyroidism and 
      developmental toxicity in larval zebrafish. These findings provided a crucial 
      aspect of the ecological risks of ILs, and presented a new insight into the 
      thyroid-disrupting mechanisms for emerging pollutants that do not have structural 
      similarity to THs.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Wei, Penghao
AU  - Wei P
AD  - School of Environmental and Municipal Engineering, Qingdao University of 
      Technology, Qingdao 266520, China; School of Environmental Science and 
      Engineering, Shandong University, Qingdao 266237, China.
FAU - Xiao, Yihua
AU  - Xiao Y
AD  - School of Environmental and Municipal Engineering, Qingdao University of 
      Technology, Qingdao 266520, China.
FAU - Liu, Changqing
AU  - Liu C
AD  - School of Environmental and Municipal Engineering, Qingdao University of 
      Technology, Qingdao 266520, China.
FAU - Yan, Bing
AU  - Yan B
AD  - Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water 
      Quality and Conservation of the Pearl River Delta, Ministry of Education, 
      Guangzhou University, Guangzhou 510006, China. Electronic address: 
      drbingyan@gzhu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230407
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (Thyroid Hormones)
SB  - IM
MH  - Animals
MH  - *Thyroid Gland
MH  - Zebrafish/metabolism
MH  - Molecular Docking Simulation
MH  - *Water Pollutants, Chemical/toxicity
MH  - Endocrine System
MH  - Thyroid Hormones/metabolism
MH  - Larva
OTO - NOTNLM
OT  - Adverse outcome pathway
OT  - Ionic liquids
OT  - Retinoid X receptors
OT  - Thyroid endocrine system
OT  - Thyroid-stimulating hormone
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/04/23 00:41
MHDA- 2023/05/15 06:42
CRDT- 2023/04/22 18:07
PHST- 2023/03/05 00:00 [received]
PHST- 2023/04/03 00:00 [revised]
PHST- 2023/04/06 00:00 [accepted]
PHST- 2023/05/15 06:42 [medline]
PHST- 2023/04/23 00:41 [pubmed]
PHST- 2023/04/22 18:07 [entrez]
AID - S0166-445X(23)00138-8 [pii]
AID - 10.1016/j.aquatox.2023.106535 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2023 Jun;259:106535. doi: 10.1016/j.aquatox.2023.106535. Epub 2023 
      Apr 7.