PMID- 37087583
OWN - NLM
STAT- MEDLINE
DCOM- 20230504
LR  - 20230504
IS  - 1673-4254 (Print)
IS  - 2663-0842 (Electronic)
IS  - 1673-4254 (Linking)
VI  - 43
IP  - 3
DP  - 2023 Mar 20
TI  - [M2 macrophage-derived exosomal lncRNA NR_028113.1 promotes macrophage 
      polarization possibly by activating the JAK2/STAT3 signaling pathway].
PG  - 393-399
LID - 10.12122/j.issn.1673-4254.2023.03.08 [doi]
AB  - OBJECTIVE: To explore the effect of M2 macrophage-derived exosomal lncRNA 
      NR_028113.1 on macrophage polarization and its possible mechanism. METHODS: Bone 
      marrow-derived macrophages (BMDMs) from BALB/c mice were isolated and cultured in 
      vitro. After IL-4 treatment to induce M2 macrophage polarization, exosomes 
      (M2-exo) were extracted from the supernatant of M2 macrophages and identified. 
      The expression of lncRNA in M2-exo was detected by qRT-PCR. BMDMs were 
      co-cultured with M2-exo (100 mug/mL) or PBS for 48 h, and the changes in cellular 
      expression levels of Arg1, YM-1, FIZZ1, iNOS and TNF-alpha were detected using 
      qRT-PCR and Western blotting. The percentage of CD206(+) cells was analyzed using 
      flow cytometry, and the phosphorylation levels of JAK2/STAT3 proteins were 
      detected using Western blotting. A lncRNA smart silencer was designed to 
      specifically inhibit the expression of lncRNA NR_028113.1 in the M2 macrophages, 
      from which exosomes were extracted and co-cultured with BMDMs for 48 h. The mRNA 
      expression levels of Arg1, YM-1, FIZZ1, iNOS and TNF-alpha, CD206(+) cell percentage 
      and the phosphorylation levels of JAK2/STAT3 proteins were detected using 
      qRT-PCR, flow cytometry and Western blotting. RESULTS: LncRNA NR_028113.1 was 
      highly expressed in the exosomes of M2 macrophages (P < 0.05). Co-culture with 
      M2-exo significantly increased mRNA expressions of M2 macrophage marker genes 
      Arg1, YM-1 and FIZZ1 (P < 0.05), lowered the expressions of iNOS and TNF-alpha (P < 
      0.05), and increased CD206(+) cell percentage and JAK2/STAT3 protein 
      phosphorylation level in BMDMs (P < 0.05). After inhibiting the expression of 
      lncRNA NR_028113.1 in M2 macrophages, the extracted M2-exo caused significant 
      down-regulation of the mRNA expressions of Arg1, YM-1 and FIZZ1 and up-regulation 
      of iNOS and TNF-alpha mRNA (P < 0.05), resulting also in signi-ficantly reduced 
      CD206(+) cell percentage and lowered phosphorylation levels of JAK2/STAT3 
      proteins in co-cultured BMDM (P < 0.05). CONCLUSIONS: M2 macrophage-derived 
      exosomal lncRNA NR_028113.1 can significantly promote M2 polarization of 
      macrophages possibly by activating the JAK2/STAT3 signaling pathway.
FAU - Zhang, M
AU  - Zhang M
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
FAU - Li, Z
AU  - Li Z
AD  - Department of Rheumatology, Yijishan Hospital, Wannan Medical College, Wuhu 
      241001, China.
FAU - Pei, W
AU  - Pei W
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
FAU - Li, X
AU  - Li X
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
FAU - Yang, H
AU  - Yang H
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
FAU - Zhu, X
AU  - Zhu X
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
FAU - Lu, K
AU  - Lu K
AD  - Key Laboratory of Non- coding RNA Transformation Research of Anhui Higher 
      Education Institution, Wannan Medical College, Wuhu 241001, China.
AD  - Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - EC 2.7.10.2 (Jak2 protein, mouse)
RN  - EC 2.7.10.2 (Janus Kinase 2)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Exosomes/genetics
MH  - Janus Kinase 2/metabolism
MH  - *Macrophage Activation/genetics
MH  - *Macrophages/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction/genetics
MH  - STAT3 Transcription Factor/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC10122731
OTO - NOTNLM
OT  - JAK2
OT  - STAT3
OT  - exosomes
OT  - long non-coding RNA
OT  - macrophage polarization
EDAT- 2023/04/23 06:41
MHDA- 2023/04/25 10:20
PMCR- 2023/03/20
CRDT- 2023/04/23 01:52
PHST- 2023/04/25 10:20 [medline]
PHST- 2023/04/23 06:41 [pubmed]
PHST- 2023/04/23 01:52 [entrez]
PHST- 2023/03/20 00:00 [pmc-release]
AID - nfykdxxb-43-3-393 [pii]
AID - 10.12122/j.issn.1673-4254.2023.03.08 [doi]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2023 Mar 20;43(3):393-399. doi: 
      10.12122/j.issn.1673-4254.2023.03.08.