PMID- 37087608
OWN - NLM
STAT- MEDLINE
DCOM- 20230425
LR  - 20230509
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 41
IP  - 5
DP  - 2023 May 8
TI  - [Determination of eight cannabinoids in foods with enhanced matrix removal-lipid 
      adsorbent by ultra performance liquid chromatography-tandem mass spectrometry].
PG  - 426-433
LID - 10.3724/SP.J.1123.2022.08010 [doi]
AB  - A novel method was developed for the simultaneous determination of eight 
      cannabinoids in six types of food matrices, including chocolate, fondant, 
      biscuit, beverage, cookie and baijiu, using ultra performance liquid 
      chromatography-tandem mass spectrometry (UPLC-MS/MS). The sample extraction and 
      cleanup steps were optimized, and various purification methods were investigated 
      to remove the oil matrix and glue in chocolate and fudge, respectively. Enhanced 
      matrix removal-lipid adsorbent (EMR-Lipid) provided efficient, selective cleanup 
      of the evaluated matrices. The sample was extracted using acetonitrile, followed 
      by EMR-Lipid cleanup, and then dried using anhydrous sodium sulfate. The 
      acetonitrile layer was concentrated by nitrogen to near-dry after 100 muL 10% 
      glycerol in methanol was added to improve the recovery by reducing loss during 
      concentration under the stream of nitrogen gas. Eight cannabinoids were separated 
      using a Waters ACQUITY UPLC BEH Shield RP18 column (100 mmx3.0 mm, 1.7 mum). The 
      responses of the cannabinoids in the positive and negative ionization modes were 
      investigated and optimized, and the responses were superior in the negative ion 
      mode compared to those in the positive ion mode. MS detection was performed in 
      the multi-reaction monitoring (MRM) mode using an electrospray source in the 
      negative ion mode. The cannabinoids were quantified using an external standard 
      with matrix calibration curves to reduce the influences of the matrix effects on 
      the quantitative results. The developed method was verified, and the conditions 
      of sample pretreatment were also optimized. The calibration curves of 
      tetrahydrocannabinol, cannabidivarin, tetrahydrocannabivarin, and cannabigerol 
      and those of cannabidiol, cannabinol, cannabidiolic acid, and 
      tetrahydrocannabinolic acid exhibited good linearities, with r>0.995, in the 
      ranges of 2-200 and 0.4-40 ng/mL, respectively. The respective limits of 
      detection (LODs, S/N=3) and quantification (LOQs, S/N=10) of 
      tetrahydrocannabinol, cannabidivarin, tetrahydrocannabivarin, and cannabigerol 
      were 4 and 10 mug/kg, and those of cannabidiol, cannabinol, cannabidiolic acid, 
      and tetrahydrocannabinolic acid were 0.8 and 2 mug/kg. The average recoveries of 
      the cannabinoids were 82.0%-114.9% under three spiked levels with corresponding 
      relative standard deviations (RSDs) of <15% (n=6). EMR-Lipid provided efficient, 
      selective cleanups of food matrices with good accuracy. The method is sensitive, 
      rapid, accurate, simple to execute, and it is suitable for the determination of 
      cannabinol compounds in typical food matrices.
FAU - Shao, Man
AU  - Shao M
AD  - Sichuan Institute of Food Inspection, Key Laboratory of Baijiu Supervising 
      Technology for State Market Regulation, Chengdu 610097, China.
FAU - Yu, Xiao-Qin
AU  - Yu XQ
AD  - Sichuan Institute of Food Inspection, Key Laboratory of Baijiu Supervising 
      Technology for State Market Regulation, Chengdu 610097, China.
FAU - Huang, Li-Juan
AU  - Huang LJ
AD  - Sichuan Institute of Food Inspection, Key Laboratory of Baijiu Supervising 
      Technology for State Market Regulation, Chengdu 610097, China.
FAU - Yao, Huan
AU  - Yao H
AD  - Sichuan Institute of Food Inspection, Key Laboratory of Baijiu Supervising 
      Technology for State Market Regulation, Chengdu 610097, China.
FAU - Li, Shu-Cai
AU  - Li SC
AD  - Sichuan Institute of Food Inspection, Key Laboratory of Baijiu Supervising 
      Technology for State Market Regulation, Chengdu 610097, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - J1K406072N (cannabigerol)
RN  - I198VBV98I (cannabidivarin)
RN  - FJX8O3OJCD (cannabidiolic acid)
RN  - 7J8897W37S (Dronabinol)
RN  - 7UYP6MC9GH (Cannabinol)
RN  - 19GBJ60SN5 (Cannabidiol)
RN  - 0 (Lipids)
SB  - IM
MH  - Chromatography, Liquid
MH  - *Tandem Mass Spectrometry/methods
MH  - Chromatography, High Pressure Liquid
MH  - Dronabinol
MH  - Cannabinol
MH  - *Cannabidiol
MH  - Lipids
PMC - PMC10122764
OTO - NOTNLM
OT  - cannabinoids
OT  - enhanced matrix removal-lipid cleaning adsorbent (EMR-Lipid)
OT  - foods
OT  - ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
EDAT- 2023/04/23 06:41
MHDA- 2023/04/25 06:42
PMCR- 2023/05/08
CRDT- 2023/04/23 03:12
PHST- 2023/04/25 06:42 [medline]
PHST- 2023/04/23 06:41 [pubmed]
PHST- 2023/04/23 03:12 [entrez]
PHST- 2023/05/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2022.08010 [doi]
PST - ppublish
SO  - Se Pu. 2023 May 8;41(5):426-433. doi: 10.3724/SP.J.1123.2022.08010.