PMID- 37087609 OWN - NLM STAT- MEDLINE DCOM- 20230425 LR - 20230509 IS - 1872-2059 (Electronic) IS - 1000-8713 (Print) IS - 1000-8713 (Linking) VI - 41 IP - 5 DP - 2023 May 8 TI - [Determination of 18 caine anesthetics in animal meat using solid phase extraction combined with ultra-performance liquid chromatography-tandem mass spectrometry]. PG - 434-442 LID - 10.3724/SP.J.1123.2022.08019 [doi] AB - Because of the widespread application of anesthetic drugs in the fields of animal breeding and transportation, demand for the rapid, sensitive detection of anesthetic drugs in animal meat is increasing. The complex animal meat matrix contains various interfering substances, such as proteins, fats, and phospholipids, along with anesthetic drug residues at very low concentrations. Therefore, adopting appropriate pretreatment methods is necessary to improve the sensitivity of detection. In this study, a rapid, accurate analytical method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and solid phase extraction (SPE) was established to determine the contents of 18 caines in animal meat. The MS parameters, such as the collision energies of 18 caines, were optimized. Furthermore, the chromatographic separation conditions and response intensities of the caine in different mobile phases were compared. The effects of different pretreatment conditions on the extraction efficiencies of the 18 caines in meat samples and those of different purification conditions, such as extraction solvent, SPE column, and dimethylsulfoxide (DMSO) dosage, on their recoveries were investigated. Combined with the external standard method, the 18 caines in meat were successfully quantified. Sample pretreatment is a three-step process. First, in ultrasound-assisted extraction, 2.0 g samples were added to 2.0 mL water and extracted using 10 mL 0.1% (v/v) formic acid in acetonitrile under ultrasound conditions for 10 min. SPE was then performed using an Oasis PRIME HLB column. Finally, DMSO-assisted concentration was employed: the organic layer was collected and dried at 40 ℃ under a stream of N(2) gas with the addition of 100 muL DMSO. Acetonitrile-water (1ratio9, v/v) was added to the residue to yield a final volume of 1.0 mL for use in UPLC-MS/MS. The 18 caines were separated using an HSS T(3) (100 mmx2.1 mm, 1.8 mum) column with 0.1% (v/v) formic acid in water (containing 0.02 mmol/L ammonium acetate) and methanol as mobile phases. Samples were detected using an electrospray ion source (ESI) in the positive ion and multiple reaction monitoring (MRM) modes during UPLC-MS/MS. Under the optimized conditions, the 18 target caine anesthetics displayed good linearities in the range of 1.00-50.0 mug/L, and the correlation coefficients (R(2)) were >0.999. The respective limits of detection (LODs) and quantification (LOQs) were 0.2-0.5 mug/kg, and 0.6-1.5 mug/kg. In pork, beef, and mutton samples, the recoveries obtained at three spiked levels were 83.4%-100.4% with relative standard deviations (RSDs) of 3.1%-8.5%. This simple, rapid, sensitive method may be applied in the detection of 18 caine anesthetics in animal meat and may provide technical support to the food safety department in China in monitoring the residues of caine anesthetics in animal meat. FAU - Wu, Shao-Ming AU - Wu SM AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Ouyang, Li-Qun AU - Ouyang LQ AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Meng, Peng AU - Meng P AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - He, Meng-Hang AU - He MH AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Lin, Qin AU - Lin Q AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Chen, Yan-Kai AU - Chen YK AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Liu, Wen-Jing AU - Liu WJ AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Su, Xiao-Ming AU - Su XM AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. FAU - Dai, Ming AU - Dai M AD - Institute of Food Inspection, Fujian Inspection and Research Institute for Product Quality, National Quality Supervision and Testing Center for Processed Food, Fuzhou 350002, China. LA - chi PT - English Abstract PT - Journal Article PL - China TA - Se Pu JT - Se pu = Chinese journal of chromatography JID - 9424804 RN - 0YIW783RG1 (formic acid) RN - YOW8V9698H (Dimethyl Sulfoxide) RN - 0 (Acetonitriles) SB - IM MH - Animals MH - Cattle MH - Chromatography, Liquid MH - Chromatography, High Pressure Liquid MH - *Tandem Mass Spectrometry MH - *Dimethyl Sulfoxide/analysis MH - Food Contamination/analysis MH - Meat/analysis MH - Solid Phase Extraction MH - Acetonitriles/analysis PMC - PMC10122766 OTO - NOTNLM OT - animal meat OT - caine anesthetics OT - solid phase extraction (SPE) OT - ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) EDAT- 2023/04/23 06:41 MHDA- 2023/04/25 06:42 PMCR- 2023/05/08 CRDT- 2023/04/23 03:12 PHST- 2023/04/25 06:42 [medline] PHST- 2023/04/23 06:41 [pubmed] PHST- 2023/04/23 03:12 [entrez] PHST- 2023/05/08 00:00 [pmc-release] AID - 10.3724/SP.J.1123.2022.08019 [doi] PST - ppublish SO - Se Pu. 2023 May 8;41(5):434-442. doi: 10.3724/SP.J.1123.2022.08019.