PMID- 37088470
OWN - NLM
STAT- MEDLINE
DCOM- 20230802
LR  - 20230802
IS  - 2055-5822 (Electronic)
IS  - 2055-5822 (Linking)
VI  - 10
IP  - 4
DP  - 2023 Aug
TI  - Rnf144b alleviates the inflammatory responses and cardiac dysfunction in sepsis.
PG  - 2338-2344
LID - 10.1002/ehf2.14383 [doi]
AB  - AIMS: Sepsis is an inflammatory disease with high mortality and morbidity. 
      Inflammation plays an essential role in sepsis, and suppressing inflammation has 
      been shown to ameliorate sepsis. Rnf144b is an ubiquitin E3 ligation with 
      anti-inflammation activities. Its precise roles in sepsis remain unknown. Here, 
      we explored the function of Rnf144b in sepsis. METHODS AND RESULTS: We generated 
      conditional knockout mice with Rnf144b deficiency in the myeloid cells. We 
      monitored the Rnf144b expression in peripheral blood mononuclear cells from 
      healthy donor and patients with sepsis, and in lipopolysaccharides (LPS)-treated 
      bone marrow-derived macrophages (BMDMs). The cytokine expression between 
      wild-type BMDMs and Rnf144b-deficient BMDMs after LPS and CpG treatments was 
      compared. The survival rate and cardiac function were monitored. The activation 
      of TANK binding kinase 1 and nuclear factor kappa-B was examined by Western blot 
      and real-time PCR. Up-regulated expression of Rnf144b was observed in peripheral 
      blood mononuclear cells from patients with sepsis. LPS induced the expression of 
      Rnf144b in BMDMs. Rnf144b-deficient BMDMs produced more inflammatory cytokines 
      after LPS or CpG stimulation. Septic mice with Rnf144b deficiency in myeloid 
      cells had higher mortality and exacerbated cardiac dysfunction. Rnf144b 
      interacted with TANK binding kinase 1 and Rnf144b deficiency resulted in impaired 
      activation of TBK1 but enhanced activation of nuclear factor kappa-B. 
      CONCLUSIONS: Rnf144b prevents inflammatory responses and cardiac dysfunction in 
      sepsis.
CI  - (c) 2023 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on 
      behalf of European Society of Cardiology.
FAU - Guo, Rennan
AU  - Guo R
AD  - Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur 
      Autonomous Region, No. 91 Tianchi Road, Urumqi, Xinjiang Uygur Autonomous Region, 
      830001, China.
FAU - Wang, Jingjing
AU  - Wang J
AD  - Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur 
      Autonomous Region, No. 91 Tianchi Road, Urumqi, Xinjiang Uygur Autonomous Region, 
      830001, China.
FAU - Tang, Wen
AU  - Tang W
AD  - Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur 
      Autonomous Region, No. 91 Tianchi Road, Urumqi, Xinjiang Uygur Autonomous Region, 
      830001, China.
FAU - Xiao, Dong
AU  - Xiao D
AD  - Department of Critical Care Medicine, People's Hospital of Xinjiang Uygur 
      Autonomous Region, No. 91 Tianchi Road, Urumqi, Xinjiang Uygur Autonomous Region, 
      830001, China.
LA  - eng
PT  - Journal Article
DEP - 20230423
PL  - England
TA  - ESC Heart Fail
JT  - ESC heart failure
JID - 101669191
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - EC 2.3.2.27 (Rnf146 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Cytokines/metabolism
MH  - *Heart Diseases
MH  - Inflammation/metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Lipopolysaccharides/metabolism/pharmacology
MH  - Macrophages/metabolism
MH  - NF-kappa B/metabolism
MH  - *Sepsis/complications/metabolism
PMC - PMC10375149
OTO - NOTNLM
OT  - Cardiac dysfunction
OT  - Inflammation
OT  - Rnf144b
OT  - Sepsis
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/04/24 00:41
MHDA- 2023/07/31 11:43
PMCR- 2023/04/23
CRDT- 2023/04/23 20:22
PHST- 2023/02/10 00:00 [revised]
PHST- 2022/10/18 00:00 [received]
PHST- 2023/04/02 00:00 [accepted]
PHST- 2023/07/31 11:43 [medline]
PHST- 2023/04/24 00:41 [pubmed]
PHST- 2023/04/23 20:22 [entrez]
PHST- 2023/04/23 00:00 [pmc-release]
AID - EHF214383 [pii]
AID - 10.1002/ehf2.14383 [doi]
PST - ppublish
SO  - ESC Heart Fail. 2023 Aug;10(4):2338-2344. doi: 10.1002/ehf2.14383. Epub 2023 Apr 
      23.