PMID- 37089022
OWN - NLM
STAT- MEDLINE
DCOM- 20230425
LR  - 20230427
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 15
IP  - 1
DP  - 2023 Jan-Dec
TI  - A distinct microbiota signature precedes the clinical diagnosis of hepatocellular 
      carcinoma.
PG  - 2201159
LID - 10.1080/19490976.2023.2201159 [doi]
LID - 2201159
AB  - Oral, gut, and tumor microbiota have been implicated as important regulators in 
      the carcinogenesis and progression of gastrointestinal malignancies. However, few 
      studies focused on the existence and association of resident microbes within 
      different body regions. Herein, we aim to reveal the durability of the 
      oral-gut-tumor microbiome and its diagnostic performance in hepatocellular 
      carcinoma (HCC). Our study included two cohorts: a retrospective discovery cohort 
      of 364 HBV-HCC patients and 160 controls with oral or fecal samples, a 
      prospective validation cohort of 91 cases, and 124 controls for matching samples, 
      as well as 48 HBV, and 39 HBV-cirrhosis patients for gut microbial patterns 
      examined by 16S rRNA gene sequencing. With the random forest analysis, 10 oral 
      and 9 gut genera that could distinguish HCC from controls in the retrospective 
      cohort were validated among the prospective matching participants, with area 
      under the curve (AUC) values of 0.7971 and 0.8084, respectively. When influential 
      taxa were merged, the AUC of the consistent classifier increased to 0.9405. The 
      performance continued to improve to 0.9811 when combined with serum levels of 
      alpha-fetoprotein (AFP). Specifically, microbial biomarkers represented by 
      Streptococcus displayed a constantly increasing trend during the disease 
      transition. Furthermore, the presence of several dominant microbiota species was 
      confirmed in hepatic tumor and non-tumor tissues with fluorescence in situ 
      hybridization (FISH) and 5 R 16S rRNA gene sequencing. Overall, our findings 
      based on the oral-gut-tumor microbiota provide a reliable approach for the early 
      detection of HCC.
FAU - Yang, Jinhuan
AU  - Yang J
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - He, Qikuan
AU  - He Q
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Lu, Fei
AU  - Lu F
AD  - The First School of Medicine, School of Information and Engineering, Wenzhou 
      Medical University, Wenzhou, China.
FAU - Chen, Kaiwen
AU  - Chen K
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Ni, ZhiHao
AU  - Ni Z
AD  - School of Nursing, Wenzhou Medical University, Wenzhou, China.
FAU - Wang, Haoyue
AU  - Wang H
AD  - School of Mental Health, Wenzhou Medical University, Wenzhou, China.
FAU - Zhou, Chen
AU  - Zhou C
AD  - The First School of Medicine, School of Information and Engineering, Wenzhou 
      Medical University, Wenzhou, China.
FAU - Zhang, Yaosheng
AU  - Zhang Y
AD  - The First School of Medicine, School of Information and Engineering, Wenzhou 
      Medical University, Wenzhou, China.
FAU - Chen, Bo
AU  - Chen B
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Bo, Zhiyuan
AU  - Bo Z
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Li, Jialiang
AU  - Li J
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Yu, Haitao
AU  - Yu H
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Epidemiology and Biostatistics, School of Public Health and 
      Management, Wenzhou Medical University; Chashan High Education Zone, Wenzhou, 
      China.
FAU - Chen, Gang
AU  - Chen G
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, China.
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, 
      Wenzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Humans
MH  - *Carcinoma, Hepatocellular/diagnosis/pathology
MH  - *Liver Neoplasms/diagnosis
MH  - Retrospective Studies
MH  - RNA, Ribosomal, 16S/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - ROC Curve
MH  - *Gastrointestinal Microbiome/genetics
MH  - *Microbiota
PMC - PMC10128432
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - AFP
OT  - Microbiota
OT  - early detection
OT  - fluorescence in situ hybridization
OT  - hepatocellular carcinoma
COIS- No potential conflict of interest was reported by the authors.
EDAT- 2023/04/24 06:42
MHDA- 2023/04/25 10:20
PMCR- 2023/04/23
CRDT- 2023/04/24 02:12
PHST- 2023/04/25 10:20 [medline]
PHST- 2023/04/24 06:42 [pubmed]
PHST- 2023/04/24 02:12 [entrez]
PHST- 2023/04/23 00:00 [pmc-release]
AID - 2201159 [pii]
AID - 10.1080/19490976.2023.2201159 [doi]
PST - ppublish
SO  - Gut Microbes. 2023 Jan-Dec;15(1):2201159. doi: 10.1080/19490976.2023.2201159.