PMID- 37090767
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230425
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 10
DP  - 2023
TI  - Oral pyruvate prevents high-intensity interval exercise-induced metabolic 
      acidosis in rats by promoting lactate dehydrogenase reaction.
PG  - 1096986
LID - 10.3389/fnut.2023.1096986 [doi]
LID - 1096986
AB  - INTRODUCTION: There is no denying the clinical benefits of exogenous pyruvate in 
      the treatment of pathological metabolic acidosis. However, whether it can prevent 
      exercise physiological metabolic acidosis, delay the occurrence of exercise 
      fatigue, and improve the beneficial effects of exercise and its internal 
      mechanism remain unclear. METHODS: We randomly divided 24 male SD rats into 3 
      groups: one group was a control without exercise (CC, n = 8), and the other two 
      groups were supplemented with 616 mg/kg/day pyruvate (EP, n = 8) or distilled 
      water of equal volume (EC, n = 8). These groups completed acute high-intensity 
      interval exercise (HIIE) after 7 days of supplementation. The acid metabolism 
      variables were measured immediately after exercise including blood pH (pH(e)), 
      base excess (BE), HCO(3) (-), blood lactic acid and skeletal muscle pH (pH(i)). 
      The redox state was determined by measuring the oxidized coenzyme I/reduced 
      coenzyme I (nicotinamide adenine dinucleotide [NAD(+)]/reduced NAD(+) [NADH]) 
      ratio and lactate/pyruvate (L/P) ratio. In addition, the activities of lactate 
      dehydrogenase A (LDHA), hexokinase (HK), phosphofructokinase (PFK) and pyruvate 
      kinase (PK) were determined by ELISA. RESULTS: Pyruvate supplementation 
      significantly reversed the decrease of pHe, BE, HCO(3) (-) and pH(i) values after 
      HIIE (p < 0.001), while significantly increased the activities of LDHA (p = 
      0.048), HK (p = 0.006), and PFK (p = 0.047). Compared with the CC, the NAD+/NADH 
      (p = 0.008) ratio and the activities of LDHA (p = 0.002), HK (p < 0.001), PFK (p 
      < 0.001), and PK (p = 0.006) were significantly improved in EP group. DISCUSSION: 
      This study provides compelling evidence that oral pyruvate attenuates 
      HIIE-induced intracellular and extracellular acidification, possibly due to 
      increased activity of LDHA, which promotes the absorption of H(+) in the LDH 
      reaction. The beneficial effects of improving the redox state and glycolysis rate 
      were also shown. Our results suggest that pyruvate can be used as an oral 
      nutritional supplement to buffer HIIE induced metabolic acidosis.
CI  - Copyright (c) 2023 Che, Yang, Zhang, Feng, Xie, Li and Qiu.
FAU - Che, Kaixuan
AU  - Che K
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Yang, Yanping
AU  - Yang Y
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Feng, Lin
AU  - Feng L
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Xie, Yan
AU  - Xie Y
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Li, Qinlong
AU  - Li Q
AD  - Department of Exercise Physiology, Exercise Science School, Beijing Sport 
      University, Beijing, China.
FAU - Qiu, Junqiang
AU  - Qiu J
AD  - Department of Exercise Biochemistry, Exercise Science School, Beijing Sport 
      University, Beijing, China.
AD  - Beijing Sports Nutrition Engineering Research Center, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20230406
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC10117856
OTO - NOTNLM
OT  - glycolysis
OT  - high-intensity interval exercise
OT  - lactate dehydrogenase reaction
OT  - metabolic acidosis
OT  - redox
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/04/24 06:41
MHDA- 2023/04/24 06:42
PMCR- 2023/01/01
CRDT- 2023/04/24 03:46
PHST- 2022/11/13 00:00 [received]
PHST- 2023/03/20 00:00 [accepted]
PHST- 2023/04/24 06:42 [medline]
PHST- 2023/04/24 06:41 [pubmed]
PHST- 2023/04/24 03:46 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2023.1096986 [doi]
PST - epublish
SO  - Front Nutr. 2023 Apr 6;10:1096986. doi: 10.3389/fnut.2023.1096986. eCollection 
      2023.