PMID- 37090801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230425
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 17
DP  - 2023
TI  - Prenatal ethanol exposure leads to persistent anxiety-like behavior during 
      adulthood indicated by reduced horizontal and vertical exploratory behaviors.
PG  - 1163575
LID - 10.3389/fnins.2023.1163575 [doi]
LID - 1163575
AB  - BACKGROUND: Fetal alcohol spectrum disorders (FASD) caused by prenatal ethanol 
      exposure (PE) consist of many cognitive/behavioral deficits. Studies have 
      reported that PE leads to impairments of learning and memory, attention, 
      executive function, and anxiety. Open field (OF) is a common behavioral model 
      which offers comprehensive ethological information. Here, we analyzed multiple 
      parameters of OF to examine anxiety behavior and habituation after PE. MATERIAL 
      AND METHODS: Pregnant Sprague Dawley rats were gavaged twice/day with 0 or 
      3 g/kg/treatment ethanol (15% w/v) during gestational day (GD) 8-20, mimicking 
      second-trimester heavy PE in humans. The control and PE adult offspring were 
      subjected to OF task in different ambient light levels with or without acute 
      stress. RESULTS: Prenatal ethanol exposure did not influence the overall 
      locomotor activities or habituation in the OF. In lower ambient light, no PE 
      effects could be detected. In higher ambient light, female PE rats showed less 
      activities in the center zone, indicative of increased anxiety. Males show lower 
      activities in the center zone only after acute stress. Rats spent <2% of the time 
      in the center zone compared to >75% of the time in the corner zone where they 
      engaged in frequent rearing activities (vertical exploration; exploratory 
      rearing). Prenatal ethanol exposure led to lower rearing activities in the corner 
      in both males and females. Acute stress masks the PE effects in males but not in 
      females. DISCUSSION: The results support that heavy PE leads to persistent 
      anxiety-like behavior during adulthood in both sexes. This conclusion is 
      supported by using multiple parameters of exploratory behavior in the OF, 
      including the rearing activities in the corner to reach reliable quantification 
      of anxiety-like behavior.
CI  - Copyright (c) 2023 Wang, Micov, Kwarteng, Wang, Hausknecht, Oubraim, Haj-Dahmane 
      and Shen.
FAU - Wang, An-Li
AU  - Wang AL
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Micov, Veronika B
AU  - Micov VB
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Kwarteng, Francis
AU  - Kwarteng F
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Wang, Ruixiang
AU  - Wang R
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Hausknecht, Kathryn A
AU  - Hausknecht KA
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Oubraim, Saida
AU  - Oubraim S
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Haj-Dahmane, Samir
AU  - Haj-Dahmane S
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
FAU - Shen, Roh-Yu
AU  - Shen RY
AD  - Department of Pharmacology and Toxicology, Jacob School of Medicine and 
      Biomedical Sciences, University at Buffalo, Buffalo, NY, United States.
LA  - eng
PT  - Journal Article
DEP - 20230406
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC10117440
OTO - NOTNLM
OT  - acute restraint stress
OT  - anxiety-like behavior
OT  - exploratory rearing
OT  - fetal alcohol spectrum disorders
OT  - open field
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/04/24 06:42
MHDA- 2023/04/24 06:43
PMCR- 2023/01/01
CRDT- 2023/04/24 03:46
PHST- 2023/02/10 00:00 [received]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/04/24 06:43 [medline]
PHST- 2023/04/24 06:42 [pubmed]
PHST- 2023/04/24 03:46 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2023.1163575 [doi]
PST - epublish
SO  - Front Neurosci. 2023 Apr 6;17:1163575. doi: 10.3389/fnins.2023.1163575. 
      eCollection 2023.