PMID- 37092326
OWN - NLM
STAT- MEDLINE
DCOM- 20230802
LR  - 20230802
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 103
IP  - 12
DP  - 2023 Sep
TI  - Identification of major and cross-reactive allergens of local freshwater snail 
      (Pila polita) and the impact of thermal and non-thermal food processing on 
      allergen stability.
PG  - 5819-5830
LID - 10.1002/jsfa.12659 [doi]
AB  - BACKGROUND: Snail allergy is rare but can be fatal. Pila polita, a freshwater 
      snail, was considered as a popular exotic food, particularly in tropical 
      countries, and consumed in processed forms. Thus, the purpose of this study was 
      to identify the major and cross-reactive allergens of P. polita and to determine 
      the impact of food processing on the allergen stability. RESULTS: Sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis fractionated raw snail extract to 
      approximately 24 protein bands, between 9 and 245 kDa. The prominent band at 
      33 kDa was detected in all raw and processed snail extracts. Immunoblotting tests 
      of the raw extract demonstrated 19 immunoglobulin E (IgE)-binding proteins, and 
      four of them, at 30, 35, 42 and 49 kDa, were revealed as the major IgE-binding 
      proteins of P. polita. Matrix-assisted laser desorption/ionization time-of-flight 
      mass spectrometry identified the 49 and 42 kDa major allergens as actin, whereas 
      the 30 and 35 kDa major allergens were identified as tropomyosin. Immunoblotting 
      revealed that the raw snail had more allergenic proteins than the processed 
      snail. The degree of allergenicity in decreasing order was raw > brine pickled> 
      boiled > roasted > fried > vinegar pickled. The presence of cross-reactivity 
      between P. polita and the shellfish tested was exhibited with either no, 
      complete, or partial inhibitions. CONCLUSION: Actin and tropomyosin were 
      identified as the major and cross-reactive allergens of P. polita among local 
      patients with snail allergy. Those major allergens are highly stable to high 
      temperatures, acidic pH, and high salt, which might played a crucial role in 
      snail allergy in Malaysia. (c) 2023 Society of Chemical Industry.
CI  - (c) 2023 Society of Chemical Industry.
FAU - Misnan, Rosmilah
AU  - Misnan R
AUID- ORCID: 0000-0002-1349-2193
AD  - Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan 
      Sultan Idris, Tanjong Malim, Perak, 35900, Malaysia.
FAU - Kamarazaman, Noor Asyikin
AU  - Kamarazaman NA
AD  - Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan 
      Sultan Idris, Tanjong Malim, Perak, 35900, Malaysia.
FAU - Sockalingam, Komathi
AU  - Sockalingam K
AD  - Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan 
      Sultan Idris, Tanjong Malim, Perak, 35900, Malaysia.
FAU - Yadzir, Zailatul Hani Mohamad
AU  - Yadzir ZHM
AD  - Disease Control Division, Ministry of Health Malaysia, Federal Government 
      Administrative Centre, Putrajaya, 62590, Malaysia.
FAU - Bakhtiar, Faizal
AU  - Bakhtiar F
AD  - Allergy and Immunology Research Centre, Institute for Medical Research (IMR), 
      National Institutes of Health (NIH), Shah Alam, Selangor, 40170, Malaysia.
FAU - Abdullah, Noormalin
AU  - Abdullah N
AD  - Allergy and Immunology Research Centre, Institute for Medical Research (IMR), 
      National Institutes of Health (NIH), Shah Alam, Selangor, 40170, Malaysia.
FAU - Arip, Masita
AU  - Arip M
AD  - Allergy and Immunology Research Centre, Institute for Medical Research (IMR), 
      National Institutes of Health (NIH), Shah Alam, Selangor, 40170, Malaysia.
LA  - eng
GR  - Ministry of Higher Education, Malaysia/
PT  - Journal Article
DEP - 20230509
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Allergens)
RN  - 0 (Tropomyosin)
RN  - 0 (Actins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Allergens/chemistry
MH  - *Food Hypersensitivity
MH  - Tropomyosin/chemistry
MH  - Actins
MH  - Immunoglobulin E
MH  - Snails
MH  - Food Handling
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fresh Water
MH  - Immunoblotting
OTO - NOTNLM
OT  - 2-DE
OT  - MALDI-TOF
OT  - Pila polita
OT  - SDS-PAGE
OT  - cross-reactivity
OT  - freshwater snail
OT  - immunoblotting
OT  - major allergen
OT  - stability
EDAT- 2023/04/24 06:42
MHDA- 2023/08/02 06:42
CRDT- 2023/04/24 05:03
PHST- 2023/04/18 00:00 [revised]
PHST- 2023/01/23 00:00 [received]
PHST- 2023/04/24 00:00 [accepted]
PHST- 2023/08/02 06:42 [medline]
PHST- 2023/04/24 06:42 [pubmed]
PHST- 2023/04/24 05:03 [entrez]
AID - 10.1002/jsfa.12659 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2023 Sep;103(12):5819-5830. doi: 10.1002/jsfa.12659. Epub 2023 
      May 9.