PMID- 37095163
OWN - NLM
STAT- MEDLINE
DCOM- 20230426
LR  - 20230724
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Apr 24
TI  - Standardized Centella asiatica (ECa 233) extract decreased pain hypersensitivity 
      development in a male mouse model of chronic inflammatory temporomandibular 
      disorder.
PG  - 6642
LID - 10.1038/s41598-023-33769-w [doi]
LID - 6642
AB  - Chronic inflammatory temporomandibular disorder (TMD) pain has a high prevalence, 
      and available nonspecific treatments have adverse side effects. ECa 233, a 
      standardized Centella asiatica extract, is highly anti-inflammatory and safe. We 
      investigated its therapeutic effects by injecting complete Freund's adjuvant 
      (CFA) into right temporomandibular joint of mice and administering either 
      ibuprofen or ECa 233 (30, 100, and 300 mg/kg) for 28 days. Inflammatory and 
      nociceptive markers, bone density, and pain hypersensitivity were examined. CFA 
      decreased ipsilateral bone density, suggesting inflammation localization, which 
      ipsilaterally caused immediate calcitonin gene-related peptide elevation in the 
      trigeminal ganglia (TG) and trigeminal subnucleus caudalis (TNC), followed by 
      late increase of NaV1.7 in TG and of p-CREB and activation of microglia in TNC. 
      Contralaterally, only p-CREB and activated microglia in TNC showed delayed 
      increase. Pain hypersensitivity, which developed early ipsilaterally, but late 
      contralaterally, was reduced by ibuprofen and ECa 233 (30 or 100 mg/kg). However, 
      ibuprofen and only 100-mg/kg ECa 233 effectively mitigated marker elevation. This 
      suggests 30-mg/kg ECa 233 was antinociceptive, whereas 100-mg/kg ECa 233 was both 
      anti-inflammatory and antinociceptive. ECa 233 may be alternatively and safely 
      used for treating chronic inflammatory TMD pain, showing an inverted U-shaped 
      dose-response relationship with maximal effect at 100 mg/kg.
CI  - (c) 2023. The Author(s).
FAU - Rotpenpian, Nattapon
AU  - Rotpenpian N
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
AD  - Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla 
      University, Songkhla, Thailand.
FAU - Wanasuntronwong, Aree
AU  - Wanasuntronwong A
AD  - Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, 
      Thailand.
FAU - Tapechum, Sompol
AU  - Tapechum S
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
FAU - Vattarakorn, Anchalee
AU  - Vattarakorn A
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
FAU - Care, Chit
AU  - Care C
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
FAU - Chindasri, Wongsathit
AU  - Chindasri W
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
FAU - Tilokskulchai, Kanokwan
AU  - Tilokskulchai K
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand.
FAU - Tantisira, Mayuree H
AU  - Tantisira MH
AD  - Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand.
FAU - Pakaprot, Narawut
AU  - Pakaprot N
AD  - Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol 
      University, 2 Srisavarindhira Bldg., 13Th Floor, Wanglang Road, Siriraj 
      Subdistrict, Bangkoknoi District, Bangkok, 10700, Thailand. 
      narawut.pak@mahidol.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230424
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (ECa 233 extract)
RN  - 0 (Centella asiatica extract)
RN  - WK2XYI10QM (Ibuprofen)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - 0 (Analgesics)
SB  - IM
EIN - Sci Rep. 2023 Jul 21;13(1):11794. PMID: 37479881
MH  - Animals
MH  - Male
MH  - *Centella
MH  - Ibuprofen
MH  - Pain
MH  - *Temporomandibular Joint Disorders
MH  - Freund's Adjuvant
MH  - Disease Models, Animal
MH  - *Hypersensitivity
MH  - Analgesics
PMC - PMC10126003
COIS- N.P. is currently an instructor at Faculty of Medicine Siriraj Hospital, Mahidol 
      University, Thailand. He has received a research grant (R016331041) from Siriraj 
      Research Fund (SRF) type 1, Faculty of Medicine Siriraj Hospital, Mahidol 
      University. Other authors declare no financial relationships or activities with 
      any organizations that might have an influence on the submitted work.
EDAT- 2023/04/25 00:41
MHDA- 2023/04/26 06:41
PMCR- 2023/04/24
CRDT- 2023/04/24 11:20
PHST- 2022/06/22 00:00 [received]
PHST- 2023/04/18 00:00 [accepted]
PHST- 2023/04/26 06:41 [medline]
PHST- 2023/04/25 00:41 [pubmed]
PHST- 2023/04/24 11:20 [entrez]
PHST- 2023/04/24 00:00 [pmc-release]
AID - 10.1038/s41598-023-33769-w [pii]
AID - 33769 [pii]
AID - 10.1038/s41598-023-33769-w [doi]
PST - epublish
SO  - Sci Rep. 2023 Apr 24;13(1):6642. doi: 10.1038/s41598-023-33769-w.