PMID- 37096864
OWN - NLM
STAT- MEDLINE
DCOM- 20230707
LR  - 20230707
IS  - 2045-7634 (Electronic)
IS  - 2045-7634 (Linking)
VI  - 12
IP  - 12
DP  - 2023 Jun
TI  - Visinin-like 1, a novel target gene of the Wnt/beta-catenin signaling pathway, is 
      involved in apoptosis resistance in colorectal cancer.
PG  - 13426-13437
LID - 10.1002/cam4.5970 [doi]
AB  - BACKGROUND: Abnormal activation of Wnt/beta-catenin signaling is associated with 
      various aspects of cancer development. This study explored the roles of novel 
      target genes of the Wnt/beta-catenin signaling pathway in cancer cells. METHODS: 
      Using the haploid chronic myelogenous leukemia cell line HAP1, RNA sequencing 
      (RNA-seq) was performed to identify genes whose expression was increased by APC 
      disruption and reversed by beta-catenin knockdown (KD). The regulatory mechanism and 
      function of one of the candidate genes was investigated in colorectal cancer 
      (CRC) cells. RESULTS: In total, 64 candidate genes whose expression was regulated 
      by Wnt/beta-catenin signaling were identified. Of these candidate genes, the 
      expression levels of six were reduced by beta-catenin KD in HCT116 CRC cells in our 
      previous microarray. One of these genes was Visinin-like 1 (VSNL1), which belongs 
      to the neuronal calcium-sensor gene family. The expression of VSNL1 was regulated 
      by the beta-catenin/TCF7L2 complex via two TCF7L2-binding elements in intron 1. 
      VSNL1 KD-induced apoptosis in VSNL1-positive CRC cells. Additionally, forced 
      expression of wild-type VSNL1, but not a myristoylation, Ca(2+) -binding, or 
      dimerization-defective mutant, suppressed the apoptosis induced by camptothecin 
      and doxorubicin in VSNL1-negative CRC cells. CONCLUSION: Our findings suggest 
      that VSNL1, a novel target gene of the Wnt/beta-catenin signaling pathway, is 
      associated with apoptosis resistance in CRC cells.
CI  - (c) 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
FAU - Tage, Hiroki
AU  - Tage H
AUID- ORCID: 0009-0003-3155-0179
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Yamaguchi, Kiyoshi
AU  - Yamaguchi K
AUID- ORCID: 0000-0003-2113-4369
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Nakagawa, Saya
AU  - Nakagawa S
AUID- ORCID: 0000-0002-5893-1663
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Kasuga, So
AU  - Kasuga S
AUID- ORCID: 0000-0002-4199-9549
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Takane, Kiyoko
AU  - Takane K
AUID- ORCID: 0000-0002-2467-1509
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Furukawa, Yoichi
AU  - Furukawa Y
AUID- ORCID: 0000-0003-0462-8631
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
FAU - Ikenoue, Tsuneo
AU  - Ikenoue T
AUID- ORCID: 0000-0001-8264-2573
AD  - Division of Clinical Genome Research, Advanced Clinical Research Center, 
      Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230425
PL  - United States
TA  - Cancer Med
JT  - Cancer medicine
JID - 101595310
RN  - 0 (beta Catenin)
RN  - 0 (visinin)
RN  - 0 (VSNL1 protein, human)
RN  - 0 (Neurocalcin)
SB  - IM
MH  - Humans
MH  - Apoptosis/genetics
MH  - beta Catenin/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - *Colorectal Neoplasms/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Wnt Signaling Pathway/genetics
MH  - *Neurocalcin/genetics
PMC - PMC10315817
OTO - NOTNLM
OT  - Visinin-like 1 (VSNL1)
OT  - Wnt/beta-catenin signaling
OT  - apoptosis
OT  - colorectal cancer
COIS- The authors have no conflict of interest to disclose.
EDAT- 2023/04/25 17:42
MHDA- 2023/07/04 06:42
PMCR- 2023/04/25
CRDT- 2023/04/25 07:44
PHST- 2023/03/14 00:00 [revised]
PHST- 2022/11/10 00:00 [received]
PHST- 2023/04/07 00:00 [accepted]
PHST- 2023/07/04 06:42 [medline]
PHST- 2023/04/25 17:42 [pubmed]
PHST- 2023/04/25 07:44 [entrez]
PHST- 2023/04/25 00:00 [pmc-release]
AID - CAM45970 [pii]
AID - 10.1002/cam4.5970 [doi]
PST - ppublish
SO  - Cancer Med. 2023 Jun;12(12):13426-13437. doi: 10.1002/cam4.5970. Epub 2023 Apr 
      25.