PMID- 37097143
OWN - NLM
STAT- MEDLINE
DCOM- 20230427
LR  - 20230427
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 73
IP  - 4
DP  - 2023 Apr
TI  - Streptomyces salinarius sp. nov., an actinomycete isolated from solar saltern 
      soil.
LID - 10.1099/ijsem.0.005863 [doi]
AB  - An actinobacterium, designated strain SS06011(T), was isolated from solar saltern 
      soil collected from Samut Sakhon province, Thailand. The taxonomic position of 
      this strain was established using the polyphasic taxonomic approach. The strain 
      produced grey aerial spore mass on International Streptomyces Project 2 seawater 
      agar that differentiated into spiral spore chains with rugose-surfaced spores. 
      Strain SS06011(T) was found to have ll-diaminopimelic acid in the cell 
      peptidoglycan. Whole-cell hydrolysates contained galactose, glucose and ribose. 
      MK-9(H(6)) and MK-9(H(4)) were major menaquinones. The major cellular fatty acids 
      comprised iso-C(16 : 0), anteiso-C(15 : 0) and anteiso-C(17 : 0). 
      Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and 
      phosphatidylinositol were detected in cells. These characteristics were 
      coincident with the typical morphological and chemotaxonomic properties of the 
      genus Streptomyces. The taxonomic affiliation at the genus level of this strain 
      could also be confirmed by its 16S rRNA gene sequence data. Strain SS06011(T) 
      showed the highest 16S rRNA gene sequence similarity to Streptomyces ardesiacus 
      NRRL B-1773(T) (99.1 %), Streptomyces coelicoflavus NBRC 15399(T) (99.1 %) and 
      Streptomyces hyderabadensis OU-40(T) (99.1 %). Digital DNA-DNA hybridization 
      (dDDH), average nucleotide identity-blast (ANIb) and average amino acid identity 
      (AAI) values between strain SS06011(T) and its closely related type strains, S. 
      ardesiacus NBRC 15402(T), S. coelicoflavus NBRC 15399(T) and S. hyderabadensis 
      JCM 17657(T), were in the range of 45.4-48.4 % (for dDDH), 90.8-91.9 % (for ANIb) 
      and 90.8-91.7 % (for AAI), respectively, which are lower than the cut-off 
      criteria for species delineation. The DNA G+C content of genomic DNA was 
      71.9 mol%. With the differences in physiological, biochemical and genotypic data, 
      strain SS06011(T) could be discriminated from its closest neighbours. Thus, 
      strain SS06011(T) should be recognized as representing a novel species of the 
      genus Streptomyces, for which the name Streptomyces salinarius sp. nov. is 
      proposed. The type strain is SS06011(T) (=TBRC 9951(T)=NBRC 113998(T)).
FAU - Klanbut, Khanungkan
AU  - Klanbut K
AD  - Department of Biology, School of Science, King Mongkut's Institute of Technology 
      Ladkrabang, Bangkok, 10520, Thailand.
AD  - Actinobacterial Research Unit, School of Science, King Mongkut's Institute of 
      Technology Ladkrabang, Bangkok, 10520, Thailand.
FAU - Rattanakavil, Thitiya
AU  - Rattanakavil T
AD  - Department of Biology, School of Science, King Mongkut's Institute of Technology 
      Ladkrabang, Bangkok, 10520, Thailand.
FAU - Duangupama, Thitikorn
AU  - Duangupama T
AD  - Department of Biology, School of Science, King Mongkut's Institute of Technology 
      Ladkrabang, Bangkok, 10520, Thailand.
FAU - Suriyachadkun, Chanwit
AU  - Suriyachadkun C
AD  - Thailand Bioresource Research Center (TBRC), National Center for Genetic 
      Engineering and Biotechnology (BIOTEC), National Science and Technology 
      Development Agency (NSTDA), Khlong Luang, Pathum Thani 12120, Thailand.
FAU - Herron, Paul Robert
AU  - Herron PR
AD  - Strathclyde Institute of Pharmacy and Biomedical Sciences, University of 
      Strathclyde, Glasgow G4 0RE, UK.
FAU - Thawai, Chitti
AU  - Thawai C
AD  - Department of Biology, School of Science, King Mongkut's Institute of Technology 
      Ladkrabang, Bangkok, 10520, Thailand.
AD  - Actinobacterial Research Unit, School of Science, King Mongkut's Institute of 
      Technology Ladkrabang, Bangkok, 10520, Thailand.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (Fatty Acids)
RN  - 0 (Phospholipids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Fatty Acids/chemistry
MH  - Phospholipids/chemistry
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Actinobacteria/genetics
MH  - Soil
MH  - Sequence Analysis, DNA
MH  - DNA, Bacterial/genetics
MH  - Base Composition
MH  - Thailand
MH  - Phylogeny
MH  - Bacterial Typing Techniques
MH  - *Streptomyces
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - Streptomyces
OT  - solar saltern soil
OT  - whole-genome analysis
EDAT- 2023/04/25 17:42
MHDA- 2023/04/27 06:42
CRDT- 2023/04/25 09:53
PHST- 2023/04/27 06:42 [medline]
PHST- 2023/04/25 17:42 [pubmed]
PHST- 2023/04/25 09:53 [entrez]
AID - 10.1099/ijsem.0.005863 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005863.