PMID- 37098653
OWN - NLM
STAT- MEDLINE
DCOM- 20230914
LR  - 20230930
IS  - 2590-3462 (Electronic)
IS  - 2590-3462 (Linking)
VI  - 4
IP  - 5
DP  - 2023 Sep 11
TI  - An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis.
PG  - 100607
LID - S2590-3462(23)00118-9 [pii]
LID - 10.1016/j.xplc.2023.100607 [doi]
LID - 100607
AB  - Quantitative disease resistance (QDR) remains the most prevalent form of plant 
      resistance in crop fields and wild habitats. Genome-wide association studies 
      (GWAS) have proved to be successful in deciphering the quantitative genetic basis 
      of complex traits such as QDR. To unravel the genetics of QDR to the devastating 
      worldwide bacterial pathogen Ralstonia solanacearum, we performed a GWAS by 
      challenging a highly polymorphic local mapping population of Arabidopsis thaliana 
      with four R. solanacearum type III effector (T3E) mutants, identified as key 
      pathogenicity determinants after a first screen on an A. thaliana core collection 
      of 25 accessions. Although most quantitative trait loci (QTLs) were highly 
      specific to the identity of the T3E mutant (ripAC, ripAG, ripAQ, and ripU), we 
      finely mapped a common QTL located on a cluster of nucleotide-binding domain and 
      leucine-rich repeat (NLR) genes that exhibited structural variation. We 
      functionally validated one of these NLRs as a susceptibility factor in response 
      to R. solanacearum, named it Bacterial Wilt Susceptibility 1 (BWS1), and cloned 
      two alleles that conferred contrasting levels of QDR. Further characterization 
      indicated that expression of BWS1 leads to suppression of immunity triggered by 
      different R. solanacearum effectors. In addition, we showed a direct interaction 
      between BWS1 and RipAC T3E, and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1 (SGT1b), 
      the latter interaction being suppressed by RipAC. Together, our results highlight 
      a putative role for BWS1 as a quantitative susceptibility factor directly 
      targeted by the T3E RipAC, mediating negative regulation of the SGT1-dependent 
      immune response.
CI  - Copyright (c) 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Demirjian, Choghag
AU  - Demirjian C
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Razavi, Narjes
AU  - Razavi N
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Yu, Gang
AU  - Yu G
AD  - Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular 
      Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
FAU - Mayjonade, Baptiste
AU  - Mayjonade B
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Zhang, Lu
AU  - Zhang L
AD  - Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular 
      Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
FAU - Lonjon, Fabien
AU  - Lonjon F
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Chardon, Fabien
AU  - Chardon F
AD  - Universite Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin 
      (IJPB), 78000 Versailles, France.
FAU - Carrere, Sebastien
AU  - Carrere S
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Gouzy, Jerome
AU  - Gouzy J
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Genin, Stephane
AU  - Genin S
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Macho, Alberto P
AU  - Macho AP
AD  - Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular 
      Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
FAU - Roux, Fabrice
AU  - Roux F
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Berthome, Richard
AU  - Berthome R
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France.
FAU - Vailleau, Fabienne
AU  - Vailleau F
AD  - LIPME, Universite de Toulouse, INRAE, CNRS, Castanet-Tolosan, France. Electronic 
      address: fabienne.vailleau@inrae.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230425
PL  - China
TA  - Plant Commun
JT  - Plant communications
JID - 101769147
RN  - 0 (Bacterial Proteins)
RN  - EC 2.4.1.- (SGT1 protein, Arabidopsis)
RN  - EC 2.4.1.- (Glucosyltransferases)
RN  - 0 (Arabidopsis Proteins)
SB  - IM
MH  - *Arabidopsis/genetics/metabolism
MH  - Bacterial Proteins/genetics
MH  - Genome-Wide Association Study
MH  - Disease Resistance/genetics
MH  - Virulence/genetics
MH  - Glucosyltransferases
MH  - *Arabidopsis Proteins/genetics
PMC - PMC10504594
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - GWAS
OT  - R gene
OT  - Ralstonia solanacearum
OT  - susceptibility
OT  - type III effectors
EDAT- 2023/04/26 06:42
MHDA- 2023/09/14 06:42
PMCR- 2023/04/25
CRDT- 2023/04/26 01:36
PHST- 2022/07/29 00:00 [received]
PHST- 2023/01/19 00:00 [revised]
PHST- 2023/04/20 00:00 [accepted]
PHST- 2023/09/14 06:42 [medline]
PHST- 2023/04/26 06:42 [pubmed]
PHST- 2023/04/26 01:36 [entrez]
PHST- 2023/04/25 00:00 [pmc-release]
AID - S2590-3462(23)00118-9 [pii]
AID - 100607 [pii]
AID - 10.1016/j.xplc.2023.100607 [doi]
PST - ppublish
SO  - Plant Commun. 2023 Sep 11;4(5):100607. doi: 10.1016/j.xplc.2023.100607. Epub 2023 
      Apr 25.