PMID- 37099541
OWN - NLM
STAT- MEDLINE
DCOM- 20230428
LR  - 20230511
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 4
DP  - 2023
TI  - Differential innate immune response of endometrial cells to porcine reproductive 
      and respiratory syndrome virus type 1 versus type 2.
PG  - e0284658
LID - 10.1371/journal.pone.0284658 [doi]
LID - e0284658
AB  - Modification of cellular and immunological events due to porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection is associated with pathogenesis in 
      lungs. PRRSV also causes female reproductive dysfunction and persistent infection 
      which can spread to fetus, stillbirth, and offspring. In this study, changes in 
      cellular and innate immune responses to PRRSV type 1 or type 2 infection, 
      including expression of PRRSV mediators, mRNA expression of Toll-like receptors 
      (TLRs) and cytokine, and cytokine secretion, were examined in primary porcine 
      glandular endometrial cells (PGE). Cell infectivity as observed by cytopathic 
      effect (CPE), PRRSV nucleocapsid proteins, and viral nucleic acids was detected 
      as early as two days post-infection (2 dpi) and persisted until 6 dpi. A higher 
      percentage of CPE and PRRSV-positive cells were observed in type 2 infections. 
      PRRSV mediator proteins, CD151, CD163, sialoadhesin (Sn), integrin and vimentin, 
      were upregulated following type 1 and type 2 infection. CD151, CD163 and Sn were 
      upregulated by type 2. In both PRRSV types, mRNA expression of TLR1 and TLR6 was 
      upregulated. However, TLR3 was upregulated by type 1, but TLR4 and TLR8 mRNA and 
      protein were downregulated by type 2 only. Interleukin (IL)-1beta, IL-6 and tumor 
      necrotic factor (TNF)-alpha were upregulated by type 2, but IL-8 was upregulated by 
      type 1. Both PRRSV type 1 and 2 stimulated IL-6 but suppressed TNF-alpha secretion. 
      In addition, IL-1beta secretion was suppressed only by type 2. These findings reveal 
      an important mechanism underlying the strategy of PRRSV infection in the 
      endometrium and associated with the viral persistence.
CI  - Copyright: (c) 2023 Lothong et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Lothong, Muttarin
AU  - Lothong M
AD  - Faculty of Veterinary Science, Department of Physiology, Chulalongkorn 
      University, Pathum Wan, Bangkok, Thailand.
FAU - Rukarcheep, Dran
AU  - Rukarcheep D
AD  - Faculty of Veterinary Science, Department of Physiology, Chulalongkorn 
      University, Pathum Wan, Bangkok, Thailand.
FAU - Wattanaphansak, Suphot
AU  - Wattanaphansak S
AD  - Faculty of Veterinary Science, Department of Veterinary Medicine, Chulalongkorn 
      University, Pathum Wan, Bangkok, Thailand.
FAU - Thammacharoen, Sumpun
AU  - Thammacharoen S
AD  - Faculty of Veterinary Science, Department of Physiology, Chulalongkorn 
      University, Pathum Wan, Bangkok, Thailand.
FAU - Deachapunya, Chatsri
AU  - Deachapunya C
AD  - Faculty of Medicine, Department of Physiology, Srinakharinwirot University, 
      Wattana, Bangkok, Thailand.
FAU - Poonyachoti, Sutthasinee
AU  - Poonyachoti S
AUID- ORCID: 0000-0003-3267-8599
AD  - Faculty of Veterinary Science, Department of Physiology, Chulalongkorn 
      University, Pathum Wan, Bangkok, Thailand.
LA  - eng
SI  - figshare/10.6084/m9.figshare.22303558.v1
SI  - figshare/10.6084/m9.figshare.22303543.v2
SI  - figshare/10.6084/m9.figshare.22308451.v4
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230426
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Interleukin-6)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Swine
MH  - Female
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/metabolism
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - Interleukin-6
MH  - Immunity, Innate
MH  - Cytokines/metabolism
MH  - Tumor Necrosis Factor-alpha
MH  - Endometrium/metabolism
MH  - RNA, Messenger/genetics/metabolism
PMC - PMC10132667
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/04/26 18:42
MHDA- 2023/04/28 06:41
PMCR- 2023/04/26
CRDT- 2023/04/26 13:43
PHST- 2022/11/30 00:00 [received]
PHST- 2023/04/04 00:00 [accepted]
PHST- 2023/04/28 06:41 [medline]
PHST- 2023/04/26 18:42 [pubmed]
PHST- 2023/04/26 13:43 [entrez]
PHST- 2023/04/26 00:00 [pmc-release]
AID - PONE-D-22-31801 [pii]
AID - 10.1371/journal.pone.0284658 [doi]
PST - epublish
SO  - PLoS One. 2023 Apr 26;18(4):e0284658. doi: 10.1371/journal.pone.0284658. 
      eCollection 2023.