PMID- 37104208
OWN - NLM
STAT- MEDLINE
DCOM- 20230501
LR  - 20230508
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 15
IP  - 4
DP  - 2023 Apr 5
TI  - Development of a Novel Magnetic-Bead-Based Automated Strategy for Efficient and 
      Low-Cost Sample Preparation for Ochratoxin A Detection Using Mycotoxin-Albumin 
      Interaction.
LID - 10.3390/toxins15040270 [doi]
LID - 270
AB  - The mycotoxin ochratoxin A (OTA) is toxic to humans and frequently contaminates 
      wine and beer. Antibodies are essential recognition probes for the detection of 
      OTA. However, they have several drawbacks, such as high costs and difficulty in 
      preparation. In this study, a novel magnetic-bead-based automated strategy for 
      efficient and low-cost OTA sample preparation was developed. Human serum albumin, 
      which is an economical and stable receptor based on the mycotoxin-albumin 
      interaction, was adapted and validated to replace conventional antibodies to 
      capture OTA in the sample. Ultra-performance liquid chromatography-fluorescence 
      detection was used in combination with this preparation method for efficient 
      detection. The effects of different conditions on this method were investigated. 
      The recovery of OTA samples spiked at three different concentrations ranged from 
      91.2% to 102.1%, and the relative standard deviations (RSDs) were 1.2%-8.2% in 
      wine and beer. For red wine and beer samples, the LODs were 0.37 and 0.15 microg/L, 
      respectively. This reliable method overcomes the drawbacks of conventional 
      methods and offers significant application prospects.
FAU - Ye, Jin
AU  - Ye J
AUID- ORCID: 0000-0001-9619-4943
AD  - Key Laboratory of Grain Information Processing and Control, Henan University of 
      Technology, Ministry of Education, Zhengzhou 450001, China.
AD  - Henan Key Laboratory of Grain Photoelectric Detection and Control, Henan 
      University of Technology, Zhengzhou 450001, China.
AD  - College of Information Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
AD  - Academy of National Food and Strategic Reserves Administration, Beijing 102600, 
      China.
FAU - Bao, Hui
AU  - Bao H
AD  - Key Laboratory of Grain Information Processing and Control, Henan University of 
      Technology, Ministry of Education, Zhengzhou 450001, China.
AD  - Henan Key Laboratory of Grain Photoelectric Detection and Control, Henan 
      University of Technology, Zhengzhou 450001, China.
AD  - College of Information Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
FAU - Zheng, Mengyao
AU  - Zheng M
AD  - Academy of National Food and Strategic Reserves Administration, Beijing 102600, 
      China.
AD  - College of Food Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
FAU - Liu, Hongmei
AU  - Liu H
AD  - Academy of National Food and Strategic Reserves Administration, Beijing 102600, 
      China.
FAU - Chen, Jinnan
AU  - Chen J
AD  - Academy of National Food and Strategic Reserves Administration, Beijing 102600, 
      China.
FAU - Wang, Songxue
AU  - Wang S
AUID- ORCID: 0000-0002-0555-5545
AD  - Academy of National Food and Strategic Reserves Administration, Beijing 102600, 
      China.
AD  - College of Food Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
FAU - Ma, Haihua
AU  - Ma H
AD  - Key Laboratory of Grain Information Processing and Control, Henan University of 
      Technology, Ministry of Education, Zhengzhou 450001, China.
AD  - Henan Key Laboratory of Grain Photoelectric Detection and Control, Henan 
      University of Technology, Zhengzhou 450001, China.
AD  - College of Information Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
FAU - Zhang, Yuan
AU  - Zhang Y
AD  - Key Laboratory of Grain Information Processing and Control, Henan University of 
      Technology, Ministry of Education, Zhengzhou 450001, China.
AD  - Henan Key Laboratory of Grain Photoelectric Detection and Control, Henan 
      University of Technology, Zhengzhou 450001, China.
AD  - College of Information Science and Engineering, Henan University of Technology, 
      Zhengzhou 450001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230405
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 1779SX6LUY (ochratoxin A)
RN  - 0 (Mycotoxins)
RN  - 0 (Ochratoxins)
RN  - 0 (Albumins)
SB  - IM
MH  - Humans
MH  - *Mycotoxins/analysis
MH  - *Ochratoxins/analysis
MH  - *Wine/analysis
MH  - Albumins
MH  - Magnetic Phenomena
MH  - Chromatography, High Pressure Liquid/methods
MH  - Food Contamination/analysis
PMC - PMC10145472
OTO - NOTNLM
OT  - albumin
OT  - automated magneto-controlled preparation
OT  - beer
OT  - mycotoxin-albumin interaction
OT  - ochratoxin A
OT  - wine
COIS- The authors declare no conflict of interest.
EDAT- 2023/04/27 18:41
MHDA- 2023/05/01 06:42
PMCR- 2023/04/05
CRDT- 2023/04/27 13:23
PHST- 2023/02/17 00:00 [received]
PHST- 2023/03/28 00:00 [revised]
PHST- 2023/04/04 00:00 [accepted]
PHST- 2023/05/01 06:42 [medline]
PHST- 2023/04/27 18:41 [pubmed]
PHST- 2023/04/27 13:23 [entrez]
PHST- 2023/04/05 00:00 [pmc-release]
AID - toxins15040270 [pii]
AID - toxins-15-00270 [pii]
AID - 10.3390/toxins15040270 [doi]
PST - epublish
SO  - Toxins (Basel). 2023 Apr 5;15(4):270. doi: 10.3390/toxins15040270.