PMID- 37105033
OWN - NLM
STAT- MEDLINE
DCOM- 20230524
LR  - 20230524
IS  - 1808-8686 (Electronic)
IS  - 1808-8694 (Print)
IS  - 1808-8686 (Linking)
VI  - 89
IP  - 3
DP  - 2023 May-Jun
TI  - Exploration of the heterogeneity and interaction of epithelial cells and 
      NK/T-cells in Laryngeal Squamous Cell Carcinoma based on single-cell RNA 
      sequencing data.
PG  - 393-400
LID - S1808-8694(23)00010-1 [pii]
LID - 10.1016/j.bjorl.2023.02.003 [doi]
AB  - OBJECTIVES: We aimed to explore the heterogeneity and differentiation 
      trajectories of epithelial cells and NK/T-cells in Laryngeal Squamous Cell 
      Carcinoma (LSCC). METHODS: We downloaded the GSE150321 data set containing LSCC01 
      and LSCC02 samples single cell RNA data from Gene Expression Omnibus. The UMAP 
      analysis was performed to identify the cell subpopulations and cell locations of 
      subpopulations. Seurat package was used to analyze the differential expression of 
      genes. The function of differential expression genes was analyzed using DAVID 
      database. The monocle2 package was used to analyze differentiation trajectories. 
      We used the CellChat package to observe the signaling pathways and 
      ligand-receptor pairs for epithelial cells and NK/T-cells. RESULTS: All the LSCC 
      cells were divided into 16 subpopulation that included 7 epithelial cell subsets, 
      3 T-cell subsets. The function analysis indicated that epithelial cells and 
      NK/T-cells mainly participated in different process, such as cell cycle, immune 
      response, and cell migration. Then, the results of differentiation trajectory 
      indicated that the ability of migration, and the activation of the immune system 
      increases, while the ability of apoptosis, and glucose metabolic process 
      decreases as pseudotime. Migration-related epithelial cells act on all T-cells 
      via the CNTN2-CNTN2 ligand-receptor pair, which suggested that CNTN2 might be an 
      important biomarker for regulating migration of epithelial cells. CONCLUSIONS: 
      Our study characterized the heterogeneity of LSCC, which provided novel insights 
      into LSCC and identified a new mechanism and target for clinical LSCC threapies. 
      EVIDENCE: IV.
CI  - Copyright (c) 2023 Associacao Brasileira de Otorrinolaringologia e Cirurgia 
      Cervico-Facial. Published by Elsevier Espana, S.L.U. All rights reserved.
FAU - Liu, Yanan
AU  - Liu Y
AD  - Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, 
      Department of Otorhinolaryngology, Harbin, Heilongjiang, PR China.
FAU - Gao, Zhiguang
AU  - Gao Z
AD  - Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, 
      Department of Otorhinolaryngology, Harbin, Heilongjiang, PR China.
FAU - Peng, Cheng
AU  - Peng C
AD  - Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, 
      Department of Otorhinolaryngology, Harbin, Heilongjiang, PR China.
FAU - Jiang, Xingli
AU  - Jiang X
AD  - Heilongjiang Provincial Hospital Affiliated to Harbin Institute of Technology, 
      Department of Otorhinolaryngology, Harbin, Heilongjiang, PR China. Electronic 
      address: jxl20210517@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230214
PL  - Brazil
TA  - Braz J Otorhinolaryngol
JT  - Brazilian journal of otorhinolaryngology
JID - 101207337
RN  - 0 (MicroRNAs)
RN  - 0 (Ligands)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - *Carcinoma, Squamous Cell/genetics/pathology
MH  - *Laryngeal Neoplasms/pathology
MH  - Ligands
MH  - *Head and Neck Neoplasms
MH  - Epithelial Cells
MH  - Sequence Analysis, RNA
MH  - Gene Expression Regulation, Neoplastic
MH  - Cell Proliferation
PMC - PMC10164759
OTO - NOTNLM
OT  - Epithelial cells
OT  - Heterogeneity
OT  - Laryngeal Squamous Cell Carcinoma
OT  - NK/T-cells
EDAT- 2023/04/28 00:42
MHDA- 2023/05/24 06:42
PMCR- 2023/02/14
CRDT- 2023/04/27 18:07
PHST- 2023/01/30 00:00 [received]
PHST- 2023/02/06 00:00 [accepted]
PHST- 2023/05/24 06:42 [medline]
PHST- 2023/04/28 00:42 [pubmed]
PHST- 2023/04/27 18:07 [entrez]
PHST- 2023/02/14 00:00 [pmc-release]
AID - S1808-8694(23)00010-1 [pii]
AID - 10.1016/j.bjorl.2023.02.003 [doi]
PST - ppublish
SO  - Braz J Otorhinolaryngol. 2023 May-Jun;89(3):393-400. doi: 
      10.1016/j.bjorl.2023.02.003. Epub 2023 Feb 14.