PMID- 37107048
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230430
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 12
IP  - 4
DP  - 2023 Mar 31
TI  - In Vitro Antibiofilm Activity of Resveratrol against Aeromonas hydrophila.
LID - 10.3390/antibiotics12040686 [doi]
LID - 686
AB  - Aeromonas hydrophila is a Gram-negative bacterium that widely exists in various 
      aquatic environments and causes septicemia in fish and humans. Resveratrol, a 
      natural polyterpenoid product, has potential chemo-preventive and antibacterial 
      properties. In this study, we investigated the effect of resveratrol on A. 
      hydrophila biofilm formation and motility. The results demonstrated that 
      resveratrol, at sub-MIC levels, can significantly inhibit the biofilm formation 
      of A. hydrophila, and the biofilm was decreased with increasing concentrations. 
      The motility assay showed that resveratrol could diminish the swimming and 
      swarming motility of A. hydrophila. Transcriptome analyses (RNA-seq) showed that 
      A. hydrophila treated with 50 and 100 mug/mL resveratrol, respectively, presented 
      230 and 308 differentially expressed genes (DEGs), including 90 or 130 
      upregulated genes and 130 or 178 downregulated genes. Among them, genes related 
      to flagellar, type IV pilus and chemotaxis were significantly repressed. In 
      addition, mRNA of virulence factors OmpA, extracellular proteases, lipases and 
      T6SS were dramatically suppressed. Further analysis revealed that the major DEGs 
      involved in flagellar assembly and bacterial chemotaxis pathways could be 
      regulated by cyclic-di-guanosine monophosphate (c-di-GMP)- and LysR-Type 
      transcriptional regulator (LTTR)-dependent quorum sensing (QS) systems. Overall, 
      our results indicate that resveratrol can inhibit A. hydrophila biofilm formation 
      by disturbing motility and QS systems, and can be used as a promising candidate 
      drug against motile Aeromonad septicemia.
FAU - Qin, Ting
AU  - Qin T
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
FAU - Chen, Kai
AU  - Chen K
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
FAU - Xi, Bingwen
AU  - Xi B
AUID- ORCID: 0000-0002-0402-7281
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Pan, Liangkun
AU  - Pan L
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
FAU - Xie, Jun
AU  - Xie J
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Lu, Liushen
AU  - Lu L
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
FAU - Liu, Kai
AU  - Liu K
AD  - Key Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries 
      Research Center, Chinese Academy of Fishery Science, Wuxi 214081, China.
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
LA  - eng
GR  - CARS-45/the China Agriculture Research System of MOF and MARA/
PT  - Journal Article
DEP - 20230331
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC10135085
OTO - NOTNLM
OT  - Aeromonas hydrophila
OT  - RNA-seq
OT  - biofilm formation
OT  - motility
OT  - quorum sensing
OT  - resveratrol
COIS- The authors declare no conflict of interest.
EDAT- 2023/04/28 06:42
MHDA- 2023/04/28 06:43
PMCR- 2023/03/31
CRDT- 2023/04/28 01:19
PHST- 2023/02/20 00:00 [received]
PHST- 2023/03/23 00:00 [revised]
PHST- 2023/03/27 00:00 [accepted]
PHST- 2023/04/28 06:43 [medline]
PHST- 2023/04/28 06:42 [pubmed]
PHST- 2023/04/28 01:19 [entrez]
PHST- 2023/03/31 00:00 [pmc-release]
AID - antibiotics12040686 [pii]
AID - antibiotics-12-00686 [pii]
AID - 10.3390/antibiotics12040686 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2023 Mar 31;12(4):686. doi: 10.3390/antibiotics12040686.