PMID- 37108441
OWN - NLM
STAT- MEDLINE
DCOM- 20230501
LR  - 20230501
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 8
DP  - 2023 Apr 14
TI  - Comparative Analysis of Molecular Functions and Biological Role of Proteins from 
      Cell-Free DNA-Protein Complexes Circulating in Plasma of Healthy Females and 
      Breast Cancer Patients.
LID - 10.3390/ijms24087279 [doi]
LID - 7279
AB  - Cell-free DNA (cfDNA) circulates in the bloodstream packed in membrane-coated 
      structures (such as apoptotic bodies) or bound to proteins. To identify proteins 
      involved in the formation of deoxyribonucleoprotein complexes circulating in the 
      blood, native complexes were isolated using affinity chromatography with 
      immobilized polyclonal anti-histone antibodies from plasma of healthy females 
      (HFs) and breast cancer patients (BCPs). It was found that the nucleoprotein 
      complexes (NPCs) from HF plasma samples contained shorter DNA fragments (~180 bp) 
      than BCP NPCs. However, the share of DNA in the NPCs from cfDNA in blood plasma 
      in HFs and BCPs did not differ significantly, as well as the share of NPC protein 
      from blood plasma total protein. Proteins were separated by SDS-PAGE and 
      identified by MALDI-TOF mass spectrometry. Bioinformatic analysis showed that in 
      the presence of a malignant tumor, the proportion of proteins involved in ion 
      channels, protein binding, transport, and signal transduction increased in the 
      composition of blood-circulating NPCs. Moreover, 58 (35%) proteins are 
      differentially expressed in a number of malignant neoplasms in the NPCs of BCPs. 
      Identified NPC proteins from BCP blood can be recommended for further testing as 
      breast cancer diagnostic/prognostic biomarkers or as being useful in developing 
      gene-targeted therapy approaches.
FAU - Tutanov, Oleg
AU  - Tutanov O
AUID- ORCID: 0000-0003-4794-6322
AD  - V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
FAU - Shefer, Aleksei
AU  - Shefer A
AUID- ORCID: 0000-0001-5369-397X
AD  - V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
FAU - Tsentalovich, Yuri
AU  - Tsentalovich Y
AUID- ORCID: 0000-0002-1380-3000
AD  - International Tomography Center, Siberian Branch of Russian Academy of Sciences, 
      630090 Novosibirsk, Russia.
FAU - Tamkovich, Svetlana
AU  - Tamkovich S
AUID- ORCID: 0000-0001-7774-943X
AD  - V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
LA  - eng
GR  - N22-25-00130/Russian Science Foundation/
PT  - Journal Article
DEP - 20230414
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Cell-Free Nucleic Acids)
RN  - 0 (Nucleoproteins)
RN  - 9007-49-2 (DNA)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Humans
MH  - Female
MH  - *Breast Neoplasms/metabolism
MH  - *Cell-Free Nucleic Acids
MH  - Nucleoproteins
MH  - DNA
MH  - Plasma/metabolism
MH  - Biomarkers, Tumor/genetics
PMC - PMC10138639
OTO - NOTNLM
OT  - MALDI-TOF mass spectrometry
OT  - breast cancer
OT  - circulating DNA
OT  - histones
OT  - nucleoprotein complexes
OT  - plasma
COIS- The authors declare no conflict of interest.
EDAT- 2023/04/28 06:42
MHDA- 2023/05/01 06:42
PMCR- 2023/04/14
CRDT- 2023/04/28 01:27
PHST- 2023/03/18 00:00 [received]
PHST- 2023/04/05 00:00 [revised]
PHST- 2023/04/12 00:00 [accepted]
PHST- 2023/05/01 06:42 [medline]
PHST- 2023/04/28 06:42 [pubmed]
PHST- 2023/04/28 01:27 [entrez]
PHST- 2023/04/14 00:00 [pmc-release]
AID - ijms24087279 [pii]
AID - ijms-24-07279 [pii]
AID - 10.3390/ijms24087279 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Apr 14;24(8):7279. doi: 10.3390/ijms24087279.