PMID- 37111284
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230501
IS  - 1424-8247 (Print)
IS  - 1424-8247 (Electronic)
IS  - 1424-8247 (Linking)
VI  - 16
IP  - 4
DP  - 2023 Apr 1
TI  - Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats.
LID - 10.3390/ph16040527 [doi]
LID - 527
AB  - The anti-inflammatory and anti-apoptotic effects of molecular hydrogen, delivered 
      as hydrogen-rich saline (HRS), on spinal cord injury was investigated. 
      Four-month-old male Sprague Dawley rats (n = 24) were classified into four 
      groups: (1) control-laminectomy only at T7-T10; (2) spinal injury-dura left 
      intact, Tator and Rivlin clip compression model applied to the spinal cord for 1 
      min, no treatment given; (3) HRS group-applied intraperitoneally (i.p.) for seven 
      days; and (4) spinal injury-HRS administered i.p. for seven days after 
      laminectomy at T7-T10 level, leaving the dura intact and applying the Tator and 
      Rivlin clip compression model to the spinal cord for 1 min. Levels of 
      interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were measured in 
      blood taken at day seven from all groups, and hematoxylin-eosin (H & E) and 
      terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) were used to 
      stain the tissue samples. IL-6 and TNF-alpha levels were significantly lower in the 
      group treated with HRS following the spinal cord injury compared to the group 
      whose spinal cord was damaged. A decrease in apoptosis was also observed. The 
      anti-inflammatory and anti-apoptotic effect of IL-6 may be a clinically useful 
      adjuvant therapy after spinal cord injury.
FAU - Kayabas, Murat
AU  - Kayabas M
AD  - Department of Neurosurgery, Faculty of Medicine, Kafkas University, 36100 Kars, 
      Turkiye.
FAU - Sahin, Levent
AU  - Sahin L
AD  - Department of Emergency Medicine, Faculty of Medicine, Kafkas University, 36100 
      Kars, Turkiye.
FAU - Makav, Mustafa
AU  - Makav M
AD  - Department of Physiology, Faculty of Veterinary, Kafkas University, 36040 Kars, 
      Turkiye.
FAU - Alwazeer, Duried
AU  - Alwazeer D
AUID- ORCID: 0000-0002-2291-1628
AD  - Department of Nutrition and Dietetics, Faculty of Health Sciences, Igdir 
      University, 76000 Igdir, Turkiye.
FAU - Aras, Levent
AU  - Aras L
AD  - Department of Neurosurgery, Faculty of Medicine, Kafkas University, 36100 Kars, 
      Turkiye.
FAU - Yigit, Serdar
AU  - Yigit S
AD  - Department of Histology and Embryology, Faculty of Medicine, Kafkas University, 
      36100 Kars, Turkiye.
FAU - LeBaron, Tyler W
AU  - LeBaron TW
AUID- ORCID: 0000-0001-9164-6728
AD  - Department of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar 
      City, UT 84720, USA.
AD  - Molecular Hydrogen Institute, Enoch, UT 84721, USA.
LA  - eng
PT  - Journal Article
DEP - 20230401
PL  - Switzerland
TA  - Pharmaceuticals (Basel)
JT  - Pharmaceuticals (Basel, Switzerland)
JID - 101238453
PMC - PMC10143771
OTO - NOTNLM
OT  - apoptosis
OT  - cytokines
OT  - hydrogen-rich saline
OT  - reactive oxygen species
OT  - spinal cord injury
COIS- The authors declare no conflict of interest.
EDAT- 2023/04/28 06:41
MHDA- 2023/04/28 06:42
PMCR- 2023/04/01
CRDT- 2023/04/28 01:45
PHST- 2023/02/14 00:00 [received]
PHST- 2023/03/16 00:00 [revised]
PHST- 2023/03/29 00:00 [accepted]
PHST- 2023/04/28 06:42 [medline]
PHST- 2023/04/28 06:41 [pubmed]
PHST- 2023/04/28 01:45 [entrez]
PHST- 2023/04/01 00:00 [pmc-release]
AID - ph16040527 [pii]
AID - pharmaceuticals-16-00527 [pii]
AID - 10.3390/ph16040527 [doi]
PST - epublish
SO  - Pharmaceuticals (Basel). 2023 Apr 1;16(4):527. doi: 10.3390/ph16040527.