PMID- 37113661
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230430
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 11
DP  - 2023
TI  - Agarose/crystalline nanocellulose (CNC) composites promote bone marrow-derived 
      mast cell integrity, degranulation and receptor expression but inhibit production 
      of de novo synthesized mediators.
PG  - 1160460
LID - 10.3389/fbioe.2023.1160460 [doi]
LID - 1160460
AB  - Introduction: Mast cells are highly granulated tissue-resident leukocytes that 
      require a three-dimensional matrix to differentiate and mediate immune responses. 
      However, almost all cultured mast cells rely on two-dimensional suspension or 
      adherent cell culture systems, which do not adequately reflect the complex 
      structure that these cells require for optimal function. Methods: Crystalline 
      nanocellulose (CNC), consisting of rod-like crystals 4-15 nm in diameter and 
      0.2-1 microm in length, were dispersed in an agarose matrix (12.5% w/v), and bone 
      marrow derived mouse mast cells (BMMC) were cultured on the agarose/CNC 
      composite. BMMC were activated with the calcium ionophore A23187 or 
      immunoglobulin E (IgE) and antigen (Ag) to crosslink high affinity IgE receptors 
      (FcepsilonRI). Results: BMMC cultured on a CNC/agarose matrix remained viable and 
      metabolically active as measured by reduction of sodium 
      3'-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) 
      benzene-sulfonic acid hydrate (XTT), and the cells maintained their membrane 
      integrity as analyzed by measuring the release of lactate dehydrogenase (LDH) and 
      propidium iodide exclusion by flow cytometry. Culture on CNC/agarose matrix had 
      no effect on BMMC degranulation in response to IgE/Ag or A23187. However, culture 
      of BMMC on a CNC/agarose matrix inhibited A23187-and IgE/Ag-activated production 
      of tumor necrosis factor (TNF) and other mediators such as IL-1beta, IL-4, IL-6, 
      IL-13, MCP-1/CCL2, MMP-9 and RANTES by as much as 95%. RNAseq analysis indicated 
      that BMMC expressed a unique and balanced transcriptome when cultured on 
      CNC/agarose. Discussion: These data demonstrate that culture of BMMCs on a 
      CNC/agarose matrix promotes cell integrity, maintains expression of surface 
      biomarkers such as FcepsilonRI and KIT and preserves the ability of BMMC to release 
      pre-stored mediators in response to IgE/Ag and A23187. However, culture of BMMC 
      on CNC/agarose matrix inhibits BMMC production of de novo synthesized mediators, 
      suggesting that CNC may be altering specific phenotypic characteristics of these 
      cells that are associated with late phase inflammatory responses.
CI  - Copyright (c) 2023 Kulka, Wagner, Cho, Alam, Santos, Jovel, Karamchand and 
      Marcet-Palacios.
FAU - Kulka, Marianna
AU  - Kulka M
AD  - Nanotechnology Research Centre, National Research Council Canada, Edmonton, AB, 
      Canada.
AD  - Department of Medical Microbiology and Immunology 6-020 Katz Group Centre, 
      University of Alberta, Edmonton, AB, Canada.
FAU - Wagner, Ashley
AU  - Wagner A
AD  - Nanotechnology Research Centre, National Research Council Canada, Edmonton, AB, 
      Canada.
FAU - Cho, Jae-Young
AU  - Cho JY
AD  - Nanotechnology Research Centre, National Research Council Canada, Edmonton, AB, 
      Canada.
FAU - Alam, Syed Benazir
AU  - Alam SB
AD  - Nanotechnology Research Centre, National Research Council Canada, Edmonton, AB, 
      Canada.
FAU - Santos, Joy Ramielle
AU  - Santos JR
AD  - Department of Medicine, University of Alberta, Edmonton, AB, Canada.
FAU - Jovel, Juan
AU  - Jovel J
AD  - The Metabolomics Innovation Centre (TMIC), 7-12 Heritage Medical Research Centre, 
      University of Alberta, Edmonton, AB, Canada.
FAU - Karamchand, Leshern
AU  - Karamchand L
AD  - Nanotechnology Research Centre, National Research Council Canada, Edmonton, AB, 
      Canada.
FAU - Marcet-Palacios, Marcelo
AU  - Marcet-Palacios M
AD  - Northern Alberta Institute of Technology, Edmonton, AB, Canada.
LA  - eng
PT  - Journal Article
DEP - 20230411
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC10126518
OTO - NOTNLM
OT  - IgE
OT  - cytokines
OT  - inflammation
OT  - mast cell
OT  - nanocellulose
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/04/28 06:41
MHDA- 2023/04/28 06:42
PMCR- 2023/01/01
CRDT- 2023/04/28 02:32
PHST- 2023/02/07 00:00 [received]
PHST- 2023/03/22 00:00 [accepted]
PHST- 2023/04/28 06:42 [medline]
PHST- 2023/04/28 06:41 [pubmed]
PHST- 2023/04/28 02:32 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1160460 [pii]
AID - 10.3389/fbioe.2023.1160460 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2023 Apr 11;11:1160460. doi: 10.3389/fbioe.2023.1160460. 
      eCollection 2023.