PMID- 37115250 OWN - NLM STAT- MEDLINE DCOM- 20230515 LR - 20230515 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 107 IP - 11 DP - 2023 Jun TI - Optimization of deposition process for a productive and cohesive bio-CaCO(3) to repair concrete existing cracks. PG - 3479-3494 LID - 10.1007/s00253-023-12531-2 [doi] AB - Microbial-induced carbonate precipitation (MICP) is being investigated to repair concrete cracks because of its good durability and compatibility with cementitious matrix. However, during the in-situ application, the repairing often lasts weeks, even months. And the strength regain is quite low. The repairing time is largely determined by the CaCO(3) yield, and the strength regain after the repair is closely related to the cohesion and bonding strength of CaCO(3) itself. Thus, the purpose of this paper is to obtain an efficient precipitation of bio-CaCO(3) with both high yield and good cohesion to improve the in-situ repairing efficiency. Firstly, the most influential factors on urease activity were screened and the precipitation kinetics were detailly investigated. The results show that the CaCO(3) with the largest yield and cohesion was obtained when the bacterial concentration was 10(7) cells/mL and the concentration of urea and calcium was both 0.5 M at 20 degrees C. This weight loss of bio-CaCO(3) was 9.24% under ultrasonic attack. Secondly, two models were established to quantify or semi-quantify the relationship between the most influential factors and the yield and cohesion of precipitates, respectively. The results showed the order of contribution for bio-CaCO(3) precipitation was calcium ions concentration > bacterial concentration > urea concentration > temperature > initial pH. According to these models, the required yield and cohesion of CaCO(3) by engineering could be obtained by adjusting affecting factors. Models were proposed for guiding the application of MICP in practical engineering. KEY POINTS: * Screened the most affecting factors on urease activity and investigated the precipitation kinetics. * Obtained optimal conditions of bio-CaCO. * Established two models in order to give some guidance for practical civil engineering. CI - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Gao, Ruixiao AU - Gao R AD - Department of Civil Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Ma, Jinhu AU - Ma J AD - Department of Civil Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Liu, Gang AU - Liu G AD - Department of Civil Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Chen, Huayu AU - Chen H AD - Department of Civil Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Wen, Jianfeng AU - Wen J AD - Anhui China Railway Engineering Materials Technology Co. Ltd, Hefei, 230041, China. FAU - Wang, Jianyun AU - Wang J AUID- ORCID: 0000-0001-9718-8633 AD - Department of Civil Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. jianyun.wang@xjtu.edu.cn. LA - eng PT - Journal Article DEP - 20230428 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - SY7Q814VUP (Calcium) RN - EC 3.5.1.5 (Urease) RN - H0G9379FGK (Calcium Carbonate) RN - 8W8T17847W (Urea) SB - IM MH - *Calcium MH - *Urease MH - Calcium Carbonate MH - Chemical Precipitation MH - Bacteria MH - Urea OTO - NOTNLM OT - Bio-CaCO3, OT - Cohesion, OT - Crack, OT - Existing concrete OT - Model, OT - Yield, EDAT- 2023/04/28 12:43 MHDA- 2023/05/15 06:42 CRDT- 2023/04/28 11:05 PHST- 2022/12/09 00:00 [received] PHST- 2023/04/15 00:00 [accepted] PHST- 2023/04/12 00:00 [revised] PHST- 2023/05/15 06:42 [medline] PHST- 2023/04/28 12:43 [pubmed] PHST- 2023/04/28 11:05 [entrez] AID - 10.1007/s00253-023-12531-2 [pii] AID - 10.1007/s00253-023-12531-2 [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2023 Jun;107(11):3479-3494. doi: 10.1007/s00253-023-12531-2. Epub 2023 Apr 28.