PMID- 3712004
OWN - NLM
STAT- MEDLINE
DCOM- 19860718
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 6
IP  - 6
DP  - 1986 Jun
TI  - Vasopressin mRNA regulation in individual hypothalamic nuclei: a northern and in 
      situ hybridization analysis.
PG  - 1685-94
AB  - The present study examines the relative levels of vasopressin (AVP) mRNA within 
      the paraventricular (PVN), supraoptic (SON), and suprachiasmatic (SCN) nuclei of 
      the rat hypothalamus, and details the rates at which these levels change over the 
      course of a 6 d salt-loading regimen. The quantitation of vasopressin mRNA was 
      achieved by using three different procedures: (1) cell-free translation in rabbit 
      reticulocyte lysate or (2) Northern analysis of poly(A)RNAs isolated from 
      micro-punch dissected SON, PVN, and SCN, and (3) in situ hybridization 
      histochemistry. The former involved the quantitative immunoprecipitation of the 
      neurophysin precursors containing arginine8-vasopressin (AVP) or oxytocin, and 
      the latter two techniques employed a radiolabeled synthetic oligodeoxynucleotide 
      complementary to the 3' region of the AVP mRNA. Both the cell-free studies and 
      the Northern gel analyses detected a sevenfold increase of AVP mRNA in the SON, a 
      fivefold increase in the PVN, and no significant change in the SCN following 6 d 
      of salt-loading. After the initiation of salt-drinking, these increases were 
      shown to occur between 24 and 48 hr in the SON and between 48 and 72 hr in the 
      PVN. The in situ hybridization studies revealed the anatomically correct 
      hybridization of either 32P- or 3H-labeled AVP oligonucleotide to magnocellular 
      perikarya within both the SON and PVN. Autoradiographic grains could be shown to 
      be confined to the cytoplasm of these cells, and could be co-localized with 
      immunoreactivity directed against the carboxy terminus of the AVP percursor. 
      Comparison of x-ray level autoradiograms of control and 6 day salt-loaded SON 
      revealed up to a sevenfold increase in specific signal in the salt-loaded 
      sections. It is concluded that the response of AVP mRNA to osmotic stimuli in the 
      three hypothalamic nuclei is heterogeneous, and that this heterogeneity can be 
      explained by separating AVP neurons into two systems: one responsible for 
      eliciting the antidiuretic actions of AVP via plasma AVP levels, and the other 
      involved in CNS activities not directly involved with antidiuresis.
FAU - Sherman, T G
AU  - Sherman TG
FAU - McKelvy, J F
AU  - McKelvy JF
FAU - Watson, S J
AU  - Watson SJ
LA  - eng
GR  - 1F 32 MH09239-01/MH/NIMH NIH HHS/United States
GR  - F32 MH09239-01/MH/NIMH NIH HHS/United States
GR  - P30 AM34933-10/AM/NIADDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (RNA, Messenger)
RN  - 11000-17-2 (Vasopressins)
SB  - IM
MH  - Animals
MH  - Cell-Free System
MH  - Hypothalamus/*metabolism
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Vasopressins/*metabolism
PMC - PMC6568715
EDAT- 1986/06/01 00:00
MHDA- 1986/06/01 00:01
PMCR- 1986/12/01
CRDT- 1986/06/01 00:00
PHST- 1986/06/01 00:00 [pubmed]
PHST- 1986/06/01 00:01 [medline]
PHST- 1986/06/01 00:00 [entrez]
PHST- 1986/12/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.06-06-01685.1986 [doi]
PST - ppublish
SO  - J Neurosci. 1986 Jun;6(6):1685-94. doi: 10.1523/JNEUROSCI.06-06-01685.1986.