PMID- 37121691
OWN - NLM
STAT- MEDLINE
DCOM- 20230502
LR  - 20230502
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 46
IP  - 5
DP  - 2023
TI  - Anti-inflammatory Activity of Gegen Decoction and Its Modulatory Mechanism on the 
      NF-kappaB and MAPK Signaling Pathways.
PG  - 647-654
LID - 10.1248/bpb.b22-00632 [doi]
AB  - Gegen Decoction as anti-inflammatory medicine is used in clinic widespread, 
      however the specific anti-inflammatory molecular mechanism of Gegen Decoction is 
      still unclear. The purpose was to study the anti-inflammatory activity of Gegen 
      Decoction in vivo and to research its anti-inflammatory molecular mechanism. The 
      content of main essential components in Gegen Decoction were determined by HPLC 
      method. The anti-inflammatory activity of Gegen Decoction was confirmed through 
      in vivo animal experiments. Furthermore, RAW 264.7 cells were stimulated by 
      lipopolysaccharides to induce inflammatory reaction, the modulatory effect of 
      Gegen Decoction on the activation process of mitogen-activated protein kinases 
      and nuclear factor-kappaB signaling pathways was investigated. The content of 
      puerarin was the highest among all the index components. Gegen Decoction 
      inhibited carrageenan-induced paw edema in rats and xylene-induced ear swelling 
      in mice. Gegen Decoction had no obvious toxicity against RAW 264.7 cells at the 
      concentrations of 10-40 mg/mL; significantly inhibited the release of nitric 
      oxide, prostaglandin E(2), tumor necrosis factor-alpha and interleukin-6; 
      down-regulated the high expression of inflammatory proteins inducible nitric 
      oxide synthase and cyclooxygenase-2. It inhibited the phosphorylation of 
      mitogen-activated protein kinases (MAPKs)/extracellular regulated protein kinases 
      (ERK)/c-Jun N-terminal kinase (JNK), the degradation of nuclear factor-kappaB 
      (NF-kappaB)/inhibitor of NF-kappaB-alpha (IkappaB-alpha) and the nuclear translocation of NF-kappaB/p65 
      into nucleus. Gegen Decoction exerts significant anti-inflammatory activity, 
      mainly by blocking the activation of both MAPKs and NF-kappaB pathway.
FAU - Gao, Yinhe
AU  - Gao Y
AD  - Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of 
      Education of China, School of Pharmacy, Yantai University.
FAU - Zhang, Yonggang
AU  - Zhang Y
AD  - Weifang Hospital of Traditional Chinese Medicine.
FAU - Zhang, Qingran
AU  - Zhang Q
AD  - Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of 
      Education of China, School of Pharmacy, Yantai University.
FAU - Song, Wenting
AU  - Song W
AD  - Weifang Hospital of Traditional Chinese Medicine.
FAU - Xu, Hui
AU  - Xu H
AD  - Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of 
      Education of China, School of Pharmacy, Yantai University.
FAU - Chen, Daquan
AU  - Chen D
AD  - Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of 
      Education of China, School of Pharmacy, Yantai University.
FAU - Zhao, Feng
AU  - Zhao F
AD  - Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of 
      Education of China, School of Pharmacy, Yantai University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (NF-kappa B)
RN  - 0 (kakkon-to)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (I-kappa B Proteins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - 0 (Lipopolysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Rats
MH  - Mice
MH  - Animals
MH  - *NF-kappa B/metabolism
MH  - *Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - I-kappa B Proteins/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Signal Transduction
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Nitric Oxide/metabolism
MH  - Cyclooxygenase 2/metabolism
OTO - NOTNLM
OT  - Gegen Decoction
OT  - anti-inflammatory
OT  - mechanism
OT  - mitogen-activated protein kinase (MAPK)
OT  - nuclear factor-kappaB (NF-kappaB)
EDAT- 2023/05/01 00:42
MHDA- 2023/05/02 06:42
CRDT- 2023/04/30 21:23
PHST- 2023/05/02 06:42 [medline]
PHST- 2023/05/01 00:42 [pubmed]
PHST- 2023/04/30 21:23 [entrez]
AID - 10.1248/bpb.b22-00632 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2023;46(5):647-654. doi: 10.1248/bpb.b22-00632.