PMID- 37123908
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230502
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 4
DP  - 2023 Apr
TI  - FIR-preconditioning promotes Akt-mTOR-exosome manufacture in cooperation with 
      MITF to boost resilience of rat bone marrow-derived stem cells.
PG  - e15003
LID - 10.1016/j.heliyon.2023.e15003 [doi]
LID - e15003
AB  - A previous study from our laboratory observed the protective effects of 
      far-infrared irradiation (FIR) on bone marrow-derived stem cells (BMSCs) against 
      oxidative stress. However, it remains unknown precisely how FIR influences BMSC 
      survival. We identify an unexpected route among the expression of MITF, BCL2, 
      mTOR, and exosome in FIR-preconditioned BMSCs. MITF siRNA demonstrated that loss 
      of MITF expression not only inhibited cell proliferation but also reduced the 
      FIR-mediated expression of mTOR, BCL2, and exosome. mTOR signaling pathways have 
      been implicated in cell growth, proliferation, and survival. We also found that 
      rapamycin, a potent and selective inhibitor of mTOR, when combined with MITF 
      siRNA, repressed FIR-mediated CD63 and BCL2 expression. In addition, 
      FIR-preconditioned BMSCs demonstrated more tolerance in multiple stressful 
      environments than untreated BMSCs. The elevated exosomes in conditioned medium 
      derived from FIR-preconditioned BMSCs also repaired H9c2 cells that sustained 
      cellular damage after subjected to an array of environmental stress conditions. 
      Taken together, these results reveal a possible mechanism about how 
      FIR-preconditioned BMSCs and its conditioned media could contribute to cellular 
      resilience during environmental changes via MITF-Akt-mTOR associated with exosome 
      manufacture. FIR preconditioning could thus complement and improve therapeutic 
      applications of BMSCs on outcomes of various disorders.
CI  - (c) 2023 Tech Unversity of Korea.
FAU - Jeong, Yun-Mi
AU  - Jeong YM
AD  - Department of Mechanical Engineering, Tech University of Korea, 237 Sangidaehak 
      Street, Si-heung City, Republic of Korea.
FAU - Kim, Weon
AU  - Kim W
AD  - Division of Cardiology, Department of Internal Medicine, Kyung Hee University 
      Hospital, Kyung Hee University, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20230407
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10130773
OTO - NOTNLM
OT  - Akt/mTOR/exosome manufacture
OT  - Bone marrow-derived mesenchymal/stromal cells
OT  - Cellular resilience
OT  - Far-infrared irradiation
OT  - MITF
COIS- All authors declare no competing interest.
EDAT- 2023/05/01 06:42
MHDA- 2023/05/01 06:43
PMCR- 2023/04/07
CRDT- 2023/05/01 03:35
PHST- 2022/06/30 00:00 [received]
PHST- 2023/03/21 00:00 [revised]
PHST- 2023/03/23 00:00 [accepted]
PHST- 2023/05/01 06:43 [medline]
PHST- 2023/05/01 06:42 [pubmed]
PHST- 2023/05/01 03:35 [entrez]
PHST- 2023/04/07 00:00 [pmc-release]
AID - S2405-8440(23)02210-7 [pii]
AID - e15003 [pii]
AID - 10.1016/j.heliyon.2023.e15003 [doi]
PST - epublish
SO  - Heliyon. 2023 Apr 7;9(4):e15003. doi: 10.1016/j.heliyon.2023.e15003. eCollection 
      2023 Apr.