PMID- 37126879
OWN - NLM
STAT- MEDLINE
DCOM- 20230512
LR  - 20230512
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1699
DP  - 2023 Jun 21
TI  - Microtrap-assisted microfluidic magnetic separation and concentration for 
      ultrasensitive immunoassays of biomarkers.
PG  - 464021
LID - S0021-9673(23)00247-9 [pii]
LID - 10.1016/j.chroma.2023.464021 [doi]
AB  - Precise and accurate quantitation of important biomarkers is significant, 
      especially in early-stage diseases diagnosis. To realized effective biosample 
      preparation and trace-level biomarker detection, a microtrap-assisted 
      microfluidic magnetic immunoassays (muMI) method was developed in this work. A 
      microtrap was fabricated inside the straight microchannel of muMI device to help 
      magnetic separation and concentration of immunocomplexes. These immunocomplexes 
      were enriched in microtrap of muMI device to accomplish selective and sensitive 
      biomarker detection. Horseradish peroxidase-labeled magnetic beads were employed 
      to evaluate assay feasibility and microtrap effect on assay sensitivity. The 
      microtrap-assisted muMI was then applied for model biomarkers detection. The 
      limits of detection of muMI were 0.025 pg/mL for monocyte chemoattractant 
      protein-1 (MCP-1) and 0.021 pg/mL for matrix metalloproteinase-9 (MMP-9), which 
      corresponded up to 2014-fold sensitivity improvement compared to their standard 
      microwell enzyme-linked immunosorbent assay (ELISA) results. In addition, the 
      selectivity and reproducibility of microtrap-assisted muMI were confirmed. In 
      clinical serum sample analysis, recoveries of 91.3%-106.7% with relative standard 
      deviations less than 6.1% were obtained for MCP-1 and MMP-9, and method accuracy 
      was verified by commercial ELISA kit. The developed muMI can accomplish ultratrace 
      biomarker detection offering practical tool for laboratorial and clinical 
      research.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Dong, Jianwei
AU  - Dong J
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China.
FAU - Li, Gongke
AU  - Li G
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China. Electronic 
      address: cesgkl@mail.sysu.edu.cn.
FAU - Xia, Ling
AU  - Xia L
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou, 510006, China. Electronic 
      address: xialing@mail.sysu.edu.cn.
FAU - Li, He
AU  - Li H
AD  - School of Chemistry, South China Normal University, Guangzhou 510006, China.
LA  - eng
PT  - Journal Article
DEP - 20230425
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - 0 (Biomarkers)
SB  - IM
MH  - *Microfluidics
MH  - *Matrix Metalloproteinase 9
MH  - Reproducibility of Results
MH  - Immunoassay/methods
MH  - Biomarkers
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Magnetic Phenomena
OTO - NOTNLM
OT  - Biomarker
OT  - Magnetic separation and concentration
OT  - Microfluidic immunoassay
OT  - Microtrap
OT  - Ultrasensitive detection
COIS- Declaration of Competing Interest The authors declare no conflicts of interest in 
      this work.
EDAT- 2023/05/01 18:42
MHDA- 2023/05/12 07:06
CRDT- 2023/05/01 18:00
PHST- 2023/02/18 00:00 [received]
PHST- 2023/04/07 00:00 [revised]
PHST- 2023/04/21 00:00 [accepted]
PHST- 2023/05/12 07:06 [medline]
PHST- 2023/05/01 18:42 [pubmed]
PHST- 2023/05/01 18:00 [entrez]
AID - S0021-9673(23)00247-9 [pii]
AID - 10.1016/j.chroma.2023.464021 [doi]
PST - ppublish
SO  - J Chromatogr A. 2023 Jun 21;1699:464021. doi: 10.1016/j.chroma.2023.464021. Epub 
      2023 Apr 25.