PMID- 37127284
OWN - NLM
STAT- MEDLINE
DCOM- 20230511
LR  - 20230515
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Print)
IS  - 1944-8244 (Linking)
VI  - 15
IP  - 18
DP  - 2023 May 10
TI  - Neural Stem Cells as Carriers of Nucleoside-Conjugated Nanogels: A New Approach 
      toward Cell-Mediated Delivery.
PG  - 21792-21803
LID - 10.1021/acsami.2c23283 [doi]
AB  - Neural stem cells (NSCs) present attractive natural drug delivery systems (DDSs). 
      Their migratory potential enables crossing of the blood-brain barrier and 
      efficient and selective accumulation near malignant cells. Here, we present the 
      potential of NSCs as DDSs for nucleoside analogue-conjugated nanogels (NGs). Two 
      different approaches were investigated: the intracellular loading and 
      extracellular cell surface decoration with NGs. For both designs, the 
      tumor-specific migratory potentials of NSCs remained unchanged; however, the 
      intracellular loading showed a shorter NG retention. The cell surface decoration 
      protocol yielded a high loading capacity of 100% after 1 h and a prolonged drug 
      retention. A redox-sensitive linker between NGs and the nucleoside analogue 
      5-ethynyl-2'-deoxycytidine (EdC) allowed a tumor environment-specific drug 
      release and its efficient and preferential incorporation into the DNA of the 
      tumor cells. Interestingly, the tumor-trafficking potentials of NSCs were 
      significantly potentiated by irradiation of tumor cells. In conclusion, this 
      study indicates the potentials of cell surface-decorated NSCs as DDSs for 
      tumor-specific release, cellular uptake, and incorporation of EdC into DNA.
FAU - Morgenroth, Agnieszka
AU  - Morgenroth A
AUID- ORCID: 0000-0002-5712-6881
AD  - Department of Nuclear Medicine, RWTH Aachen University, 52074 Aachen, Germany.
FAU - Baazaoui, Fatima
AU  - Baazaoui F
AD  - Department of Nuclear Medicine, RWTH Aachen University, 52074 Aachen, Germany.
FAU - Hosseinnejad, Aisa
AU  - Hosseinnejad A
AD  - DWI - Leibniz-Institute for Interactive Materials, RWTH Aachen University, 52074 
      Aachen, Germany.
FAU - Schafer, Laura
AU  - Schafer L
AD  - Department of Nuclear Medicine, RWTH Aachen University, 52074 Aachen, Germany.
FAU - Vogg, Andreas
AU  - Vogg A
AD  - Department of Nuclear Medicine, RWTH Aachen University, 52074 Aachen, Germany.
FAU - Singh, Smriti
AU  - Singh S
AUID- ORCID: 0000-0002-2164-9912
AD  - DWI - Leibniz-Institute for Interactive Materials, RWTH Aachen University, 52074 
      Aachen, Germany.
AD  - Max Planck Institute for Medical Research, Heidelberg 69120, Germany.
FAU - Mottaghy, Felix M
AU  - Mottaghy FM
AUID- ORCID: 0000-0002-7212-6521
AD  - Department of Nuclear Medicine, RWTH Aachen University, 52074 Aachen, Germany.
AD  - Department of Nuclear Medicine, Maastricht University Medical Centre, 6229 HX 
      Maastricht, Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20230501
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Nanogels)
RN  - 0 (Nucleosides)
SB  - IM
MH  - Humans
MH  - Nanogels
MH  - Nucleosides
MH  - Drug Delivery Systems
MH  - *Neoplasms
MH  - *Neural Stem Cells
PMC - PMC10176478
OTO - NOTNLM
OT  - drug delivery
OT  - glioblastoma
OT  - nanogels
OT  - neural stem cells
OT  - nucleoside
COIS- The authors declare no competing financial interest.
EDAT- 2023/05/02 00:42
MHDA- 2023/05/11 06:42
PMCR- 2023/05/12
CRDT- 2023/05/01 19:43
PHST- 2023/05/11 06:42 [medline]
PHST- 2023/05/02 00:42 [pubmed]
PHST- 2023/05/01 19:43 [entrez]
PHST- 2023/05/12 00:00 [pmc-release]
AID - 10.1021/acsami.2c23283 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2023 May 10;15(18):21792-21803. doi: 
      10.1021/acsami.2c23283. Epub 2023 May 1.