PMID- 37129704
OWN - NLM
STAT- MEDLINE
DCOM- 20230605
LR  - 20230612
IS  - 1874-270X (Electronic)
IS  - 1874-2718 (Print)
IS  - 1874-270X (Linking)
VI  - 17
IP  - 1
DP  - 2023 Jun
TI  - (1)H, (13)C, (15)N backbone chemical shift assignments of the extended ARID 
      domain in human AT-rich interactive domain protein 5a (Arid5a).
PG  - 121-127
LID - 10.1007/s12104-023-10130-w [doi]
AB  - The family of AT-rich interactive domain (ARID) containing proteins -Arids- 
      contains 15 members that have almost exclusively been described as DNA-binding 
      proteins. Interestingly, a decade ago the family member Arid5a was found to bind 
      and stabilize mRNAs of immune system key players and thereby account for driving 
      inflammatory and autoimmune diseases. How exactly binding to DNA and RNA is 
      coordinated by the Arid5a ARID domain remains unknown, mainly due to the lack of 
      atom-resolved information on nucleic acid-binding. This in particular applies to 
      the protein's ARID domain, despite the comfortable size of its core unit for 
      NMR-based investigations. Furthermore, the core domain of ARID domains is found 
      to be extended by functionally relevant, often flexible stretches, but whether 
      such elongations are present and crucial for the versatile Arid5a functions is 
      unknown. We here provide a near-complete NMR backbone resonance assignment of the 
      Arid5a ARID domain with N- and C-terminal extensions, which serves as a basis for 
      further studies of its nucleic acid-binding preferences and targeted inhibition 
      by means of NMR. Our data thus significantly contribute to unravelling mechanisms 
      of Arid5a-mediated gene regulation and diseases.
CI  - (c) 2023. The Author(s).
FAU - von Ehr, Julian
AU  - von Ehr J
AUID- ORCID: 0000-0003-4535-661X
AD  - Institute for Molecular Biosciences and Biomolecular Resonance Center (BMRZ) of 
      Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438, Frankfurt am Main, 
      Germany.
AD  - IMPRS on Cellular Biophysics, Max-von-Laue-Str. 7-9, 60438, Frankfurt am Main, 
      Germany.
FAU - Korn, Sophie Marianne
AU  - Korn SM
AUID- ORCID: 0000-0003-3798-3277
AD  - Institute for Molecular Biosciences and Biomolecular Resonance Center (BMRZ) of 
      Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438, Frankfurt am Main, 
      Germany.
FAU - Weiss, Lena
AU  - Weiss L
AD  - Institute for Molecular Biosciences and Biomolecular Resonance Center (BMRZ) of 
      Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438, Frankfurt am Main, 
      Germany.
FAU - Schlundt, Andreas
AU  - Schlundt A
AUID- ORCID: 0000-0003-2254-7560
AD  - Institute for Molecular Biosciences and Biomolecular Resonance Center (BMRZ) of 
      Goethe University Frankfurt, Max-von-Laue-Str. 7-9, 60438, Frankfurt am Main, 
      Germany. schlundt@bio.uni-frankfurt.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230502
PL  - Netherlands
TA  - Biomol NMR Assign
JT  - Biomolecular NMR assignments
JID - 101472371
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nucleic Acids)
RN  - 0 (ARID5A protein, human)
SB  - IM
MH  - Humans
MH  - *DNA-Binding Proteins/chemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - *Nucleic Acids
PMC - PMC10232636
OTO - NOTNLM
OT  - ARID domain
OT  - AT-rich interactive domain containing proteins
OT  - Helix-turn-helix motif
OT  - Inflammatory diseases
OT  - Nucleic acid-binding
OT  - Transcription regulation
COIS- The authors declare no competing interests.
EDAT- 2023/05/02 12:44
MHDA- 2023/06/02 06:42
PMCR- 2023/05/02
CRDT- 2023/05/02 11:07
PHST- 2023/02/25 00:00 [received]
PHST- 2023/04/22 00:00 [accepted]
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/05/02 12:44 [pubmed]
PHST- 2023/05/02 11:07 [entrez]
PHST- 2023/05/02 00:00 [pmc-release]
AID - 10.1007/s12104-023-10130-w [pii]
AID - 10130 [pii]
AID - 10.1007/s12104-023-10130-w [doi]
PST - ppublish
SO  - Biomol NMR Assign. 2023 Jun;17(1):121-127. doi: 10.1007/s12104-023-10130-w. Epub 
      2023 May 2.