PMID- 37132100
OWN - NLM
STAT- MEDLINE
DCOM- 20230719
LR  - 20230727
IS  - 1474-9726 (Electronic)
IS  - 1474-9718 (Print)
IS  - 1474-9718 (Linking)
VI  - 22
IP  - 7
DP  - 2023 Jul
TI  - Generalized low levels of serum N-glycans associate with better health status.
PG  - e13855
LID - 10.1111/acel.13855 [doi]
LID - e13855
AB  - Caloric restriction (CR) can prolong life and ameliorate age-related diseases; 
      thus, its molecular basis might provide new insights for finding biomarker and 
      intervention for aging and age-related disease. Glycosylation is an important 
      post-translational modification, which can timely reflect the changes of 
      intracellular state. Serum N-glycosylation was found changed with aging in humans 
      and mice. CR is widely accepted as an effective anti-aging intervention in mice 
      and could affect mouse serum fucosylated N-glycans. However, the effect of CR on 
      the level of global N-glycans remains unknown. In order to explore whether CR 
      affect the level of global N-glycans, we performed a comprehensive serum glycome 
      profiling in mice of 30% calorie restriction group and ad libitum group at 7 time 
      points across 60 weeks by MALDI-TOF-MS. At each time point, the majority of 
      glycans, including galactosylated and high mannose glycans, showed a consistent 
      low level in CR group. Interestingly, O-acetylated sialoglycans presented an 
      upward change different from other derived traits, which is mainly reflected in 
      two biantennary alpha2,6-linked sialoglycans (H5N4Ge2Ac1, H5N4Ge2Ac2). Liver 
      transcriptome analysis further revealed a decreased transcriptional level of 
      genes involved in N-glycan biosynthesis while increased level of acetyl-CoA 
      production. This finding is consistent with changes in serum N-glycans and 
      O-acetylated sialic acids. Therefore, we provided one possible molecular basis 
      for the beneficial effect of CR from N-glycosylation perspective.
CI  - (c) 2023 The Authors. Aging Cell published by Anatomical Society and John Wiley & 
      Sons Ltd.
FAU - Fan, Jiteng
AU  - Fan J
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Sha, Jichen
AU  - Sha J
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Chang, Shuwai
AU  - Chang S
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Zhao, Huijuan
AU  - Zhao H
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Niu, Xiaoyun
AU  - Niu X
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Gu, Yong
AU  - Gu Y
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Gu, Jianxin
AU  - Gu J
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
FAU - Ren, Shifang
AU  - Ren S
AUID- ORCID: 0000-0001-8931-9293
AD  - NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and 
      Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230502
PL  - England
TA  - Aging Cell
JT  - Aging cell
JID - 101130839
RN  - 0 (Sialic Acids)
RN  - 0 (Biomarkers)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - Glycosylation
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - *Sialic Acids
MH  - Biomarkers
MH  - *Polysaccharides
PMC - PMC10352567
OTO - NOTNLM
OT  - N-glycans
OT  - O-acetylation
OT  - caloric restriction
OT  - liver
OT  - serum
OT  - transcriptome
COIS- The authors declare no conflict of interest.
EDAT- 2023/05/03 06:42
MHDA- 2023/07/19 06:42
PMCR- 2023/05/02
CRDT- 2023/05/03 02:33
PHST- 2023/04/05 00:00 [revised]
PHST- 2022/12/06 00:00 [received]
PHST- 2023/04/10 00:00 [accepted]
PHST- 2023/07/19 06:42 [medline]
PHST- 2023/05/03 06:42 [pubmed]
PHST- 2023/05/03 02:33 [entrez]
PHST- 2023/05/02 00:00 [pmc-release]
AID - ACEL13855 [pii]
AID - 10.1111/acel.13855 [doi]
PST - ppublish
SO  - Aging Cell. 2023 Jul;22(7):e13855. doi: 10.1111/acel.13855. Epub 2023 May 2.