PMID- 371372
OWN - NLM
STAT- MEDLINE
DCOM- 19790523
LR  - 20061115
IS  - 0301-0546 (Print)
IS  - 0301-0546 (Linking)
VI  - 6
IP  - 2
DP  - 1978 Mar-Apr
TI  - Variation in the measurement of serum IgE levels.
PG  - 127-32
AB  - Three assays for immunoglobulin E (IgE) are compared using seven serum samples 
      covering a wide range of values. The assays examined are the double antibody 
      radioimmunoassay, single antibody/ammonium sulfate precipitation method and the 
      paper radioimmunosorbent test (PRIST). In addition, values from the double 
      antibody and single antibody/ammonium sulfate assays are compared using two 
      different antisera to IgE. PRIST results in consistently lower values but the 
      three assays produce results that would not have been clinically significantly 
      different. The range of values obtained over a six-month period with aliquots of 
      the same two samples run in duplicate twenty-three times in the double antibody 
      assay was 1080 to 1440 I.U./ml for the Hi sample and 5.4 to 9.0 I.U./ml for the 
      LO. Results are compared to those of a national study in which serum samples were 
      supplied blind to research laboratories for analysis by two methods. Coefficients 
      of variation from 35% to 73% are unacceptably large and indicate the need to 
      establish international standards for serum IgE quantitation.
FAU - Wilcsek, R J
AU  - Wilcsek RJ
FAU - Hamburger, R N
AU  - Hamburger RN
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - Immunoglobulin E/*analysis/*metabolism
MH  - Immunosorbent Techniques
MH  - Methods
EDAT- 1978/03/01 00:00
MHDA- 1978/03/01 00:01
CRDT- 1978/03/01 00:00
PHST- 1978/03/01 00:00 [pubmed]
PHST- 1978/03/01 00:01 [medline]
PHST- 1978/03/01 00:00 [entrez]
PST - ppublish
SO  - Allergol Immunopathol (Madr). 1978 Mar-Apr;6(2):127-32.