PMID- 37139641
OWN - NLM
STAT- MEDLINE
DCOM- 20230721
LR  - 20230723
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 9
IP  - 4
DP  - 2023 Jul
TI  - Reference interval, longitudinal variability and reliability of activated 
      clotting time in healthy dogs using a point-of-care analyser.
PG  - 1534-1540
LID - 10.1002/vms3.1148 [doi]
AB  - BACKGROUND: Activated clotting times (ACTs) are used to screen for coagulopathies 
      and monitor heparin therapy. OBJECTIVES: To determine a reference interval (RI) 
      for ACT in dogs using a point-of-care analyser, to quantify intra-subject within- 
      and between-day variability, to quantify analyser reliability and inter-analyser 
      agreement and to study the influence of a delay in measurement. METHODS: 
      Forty-two healthy dogs were included. Measurements were performed on fresh venous 
      blood using the i-STAT 1 analyser. The RI was determined using the Robust method. 
      Intra-subject within-day variability and between-day variability were quantified 
      between baseline and 2 h (n = 8) or 48 h (n = 10) later. Analyser reliability and 
      inter-analyser agreement were studied by duplicate measurements (n = 8) on 
      identical analysers. The influence of measurement delay was studied before and 
      after a delay of one analytical run (n = 6). RESULTS: Mean, lower and upper 
      reference limits for ACT were 92.9 +/- 9.1, 74.4 and 111.2 s, respectively. 
      Coefficients of variation of intra-subject within- and between-day variability 
      were 8.1% and 10.4%, respectively, resulting in a significant between-day 
      measurement difference. Analyser reliability assessed by the intraclass 
      correlation coefficient and coefficient of variation were 0.87% and 3.3%, 
      respectively. Significantly lower ACT values were observed after a measurement 
      delay compared to direct analysis. CONCLUSIONS: Our study provides an RI for ACT 
      in healthy dogs using the i-STAT 1 and suggests low intra-subject within- and 
      between-day variability. Analyser reliability and inter-analyser agreement were 
      good; however, analysis delay and between-day differences could significantly 
      influence ACT results.
CI  - (c) 2023 The Authors. Veterinary Medicine and Science published by John Wiley & 
      Sons Ltd.
FAU - Hellemans, Arnaut
AU  - Hellemans A
AUID- ORCID: 0000-0002-5373-3131
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Devriendt, Nausikaa
AU  - Devriendt N
AUID- ORCID: 0000-0002-5070-8176
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - De Somer, Filip
AU  - De Somer F
AD  - Department of Cardiac Surgery, Faculty of Medical Sciences, Ghent University, 
      Ghent, Belgium.
AD  - Experimental Research Laboratory of Cardiac Surgery and Circulatory Physiology, 
      Faculty of Medical Sciences, Ghent University, Ghent, Belgium.
FAU - Marynissen, Sofie
AU  - Marynissen S
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Daminet, Sylvie
AU  - Daminet S
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Paepe, Dominique
AU  - Paepe D
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
FAU - Smets, Pascale
AU  - Smets P
AD  - Small Animal Department, Faculty of Veterinary Medicine, Ghent University, 
      Merelbeke, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230504
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
SB  - IM
MH  - Dogs
MH  - Animals
MH  - *Point-of-Care Systems
MH  - Reproducibility of Results
PMC - PMC10357262
OTO - NOTNLM
OT  - bedside testing
OT  - canine
OT  - coagulation
OT  - heparin monitoring
OT  - i-STAT 1
OT  - screening
COIS- The authors declare no conflicts of interest.
EDAT- 2023/05/04 06:42
MHDA- 2023/07/21 06:42
PMCR- 2023/05/04
CRDT- 2023/05/04 03:43
PHST- 2023/02/19 00:00 [revised]
PHST- 2022/09/07 00:00 [received]
PHST- 2023/04/11 00:00 [accepted]
PHST- 2023/07/21 06:42 [medline]
PHST- 2023/05/04 06:42 [pubmed]
PHST- 2023/05/04 03:43 [entrez]
PHST- 2023/05/04 00:00 [pmc-release]
AID - VMS31148 [pii]
AID - 10.1002/vms3.1148 [doi]
PST - ppublish
SO  - Vet Med Sci. 2023 Jul;9(4):1534-1540. doi: 10.1002/vms3.1148. Epub 2023 May 4.