PMID- 37139781
OWN - NLM
STAT- Publisher
LR  - 20230504
IS  - 1947-6043 (Electronic)
IS  - 1947-6035 (Linking)
DP  - 2023 May 4
TI  - Mesenchymal Stem Cell-Derived Extracellular Vesicles Protect Rat Nucleus Pulposus 
      Cells from Oxidative Stress.
PG  - 19476035231172154
LID - 10.1177/19476035231172154 [doi]
AB  - BACKGROUND: Oxidative stress (OS) is mainly associated with the pathogenesis of 
      intervertebral disc (IVD) degeneration; it causes nucleus pulposus cells (NPCs) 
      to undergo senescence and triggers autophagy and apoptosis. This study aims to 
      evaluate the regeneration potential of extracellular vesicles (EVs) derived from 
      human umbilical cord-mesenchymal stem cells (hUC-MSCs) in an in vitro rat 
      NPC-induced OS model. DESIGN: NPCs were isolated from rat coccygeal discs, 
      propagated, and characterized. OS was induced by hydrogen peroxide (H(2)O(2)), 
      which is confirmed by 2,7-dichlorofluorescein diacetate (H(2)DCFDA) assay. EVs 
      were isolated from hUC-MSCs and characterized by analyzing the vesicles using 
      fluorescence microscope, scanning electron microscope (SEM), atomic force 
      microscope (AFM), dynamic light scattering (DLS), and Western blot (WB). The in 
      vitro effects of EVs on migration, uptake, and survival of NPCs were determined. 
      RESULTS: SEM and AFM topographic images revealed the size distribution of EVs. 
      The phenotypes of isolated EVs showed that the size of EVs was 403.3 +/- 85.94 nm, 
      and the zeta potential was -0.270 +/- 4.02 mV. Protein expression analysis showed 
      that EVs were positive for CD81 and annexin V. Treatment of NPCs with EVs reduced 
      H(2)O(2)-induced OS as evidenced by a decrease in reactive oxygen species (ROS) 
      levels. Co-culture of NPCs with DiI-labeled EVs showed the cellular 
      internalization of EVs. In the scratch assay, EVs significantly increased NPC 
      proliferation and migration toward the scratched area. Quantitative polymerase 
      chain reaction analysis showed that EVs significantly reduced the expression of 
      OS genes. CONCLUSION: EVs protected NPCs from H(2)O(2)-induced OS by reducing 
      intracellular ROS generation and improved NPC proliferation and migration.
FAU - Ekram, Sobia
AU  - Ekram S
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Khalid, Shumaila
AU  - Khalid S
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Ramzan, Faiza
AU  - Ramzan F
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Salim, Asmat
AU  - Salim A
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Bashir, Imtiaz
AU  - Bashir I
AD  - University of Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, Pessac, France.
FAU - Durrieu, Marie Christine
AU  - Durrieu MC
AD  - Zainab Panjwani Memorial Hospital, Karachi, Pakistan.
FAU - Khan, Irfan
AU  - Khan I
AUID- ORCID: 0000-0003-1878-7836
AD  - Dr. Panjwani Center for Molecular Medicine and Drug Research, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20230504
PL  - United States
TA  - Cartilage
JT  - Cartilage
JID - 101518378
SB  - IM
OTO - NOTNLM
OT  - extracellular vesicles
OT  - human umbilical cord
OT  - intervertebral discs
OT  - mesenchymal stem cells
OT  - nucleus pulposus
OT  - oxidative stress
OT  - reactive oxygen species
EDAT- 2023/05/04 06:42
MHDA- 2023/05/04 06:42
CRDT- 2023/05/04 04:43
PHST- 2023/05/04 06:42 [medline]
PHST- 2023/05/04 06:42 [pubmed]
PHST- 2023/05/04 04:43 [entrez]
AID - 10.1177/19476035231172154 [doi]
PST - aheadofprint
SO  - Cartilage. 2023 May 4:19476035231172154. doi: 10.1177/19476035231172154.