PMID- 37140882
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20230602
IS  - 1573-7373 (Electronic)
IS  - 0167-594X (Linking)
VI  - 163
IP  - 1
DP  - 2023 May
TI  - Evidence for an intra-tumoral microbiome in pituitary neuroendocrine tumors with 
      different clinical phenotypes.
PG  - 133-142
LID - 10.1007/s11060-023-04318-2 [doi]
AB  - PURPOSE: Bacteria have been observed in the tumor environment for decades and 
      have been demonstrated to play important roles in the pathogenesis and 
      development of several different tumors. So far there is a clear lack of specific 
      studies relating to the presence of bacteria in pituitary neuroendocrine tumors 
      (PitNETs). METHODS: In this study, we performed five region-based amplification 
      and bacterial 16 S rRNA sequencing to identify the microbiome of PitNET tissues 
      across four clinical phenotypes. Multiple filter procedures were performed to 
      inhibit the risk of contamination with bacteria and bacterial DNA. Histological 
      analysis was also conducted to validate the localization of bacteria in the 
      intra-tumoral region. RESULTS: We identified common and diverse bacterial types 
      across the four clinical phenotypes of PitNET. We also predicted the potential 
      functions of these bacteria in tumor phenotypes and found that these functions 
      were reported in certain previous mechanistic studies. Our data indicate that the 
      pathogenesis and development of tumors may correlate with the behavior of 
      intra-tumoral bacteria. Histological results, including lipopolysaccharide (LPS) 
      staining and fluorescence in situ hybridization (FISH) for bacterial 16 S rRNA 
      clearly demonstrated the localization of bacteria in the intra-tumoral region. 
      Staining for Iba-1 suggested that the proportion of microglia was more abundant 
      in FISH-positive regions than in FISH-negative regions. Furthermore, in 
      FISH-positive regions, the microglia exhibited a longitudinally branched 
      morphology that was different to the compact morphology observed in FISH-negative 
      regions. CONCLUSION: In summary, we provide an evidence for the existence of 
      intra-tumoral bacteria in PitNET.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Ye, Lei
AU  - Ye L
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Wu, Bing-Shan
AU  - Wu BS
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Xu, Zi-Ao
AU  - Xu ZA
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Ji, Xue-Fei
AU  - Ji XF
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Guan, Liao
AU  - Guan L
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Li, Ping-Ping
AU  - Li PP
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China.
FAU - Cheng, Hong-Wei
AU  - Cheng HW
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China. hongwei.cheng@ahmu.edu.cn.
FAU - Xiao, Jin
AU  - Xiao J
AD  - Department of Neurosurgery, the First Affiliated Hospital of Anhui Medical 
      University, Jixi Road 218, Hefei, 230022, P.R. China. xjtulip@163.com.
LA  - eng
GR  - 81901238/National Natural Science Foundation of China/
GR  - KJ2019A0248/University Natural Science Research Project of Anhui Province/
GR  - 2022xkj144/Scientific Foundation of Anhui Medical University/
GR  - 2021lcxk017/Co-construction Project of Anhui Medical University and Affiliated 
      Hospital/
PT  - Journal Article
DEP - 20230504
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
SB  - IM
MH  - Humans
MH  - *Neuroendocrine Tumors
MH  - In Situ Hybridization, Fluorescence
MH  - *Pituitary Neoplasms/pathology
MH  - Pituitary Gland/pathology
MH  - *Microbiota
OTO - NOTNLM
OT  - Histology
OT  - Intra-tumoral
OT  - Microbiome
OT  - Pituitary adenomas
EDAT- 2023/05/04 12:42
MHDA- 2023/06/02 06:42
CRDT- 2023/05/04 11:16
PHST- 2023/03/17 00:00 [received]
PHST- 2023/04/19 00:00 [accepted]
PHST- 2023/06/02 06:42 [medline]
PHST- 2023/05/04 12:42 [pubmed]
PHST- 2023/05/04 11:16 [entrez]
AID - 10.1007/s11060-023-04318-2 [pii]
AID - 10.1007/s11060-023-04318-2 [doi]
PST - ppublish
SO  - J Neurooncol. 2023 May;163(1):133-142. doi: 10.1007/s11060-023-04318-2. Epub 2023 
      May 4.