PMID- 37142453
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240418
IS  - 1878-3562 (Electronic)
IS  - 1590-8658 (Linking)
VI  - 55
IP  - 12
DP  - 2023 Dec
TI  - Elaboration of NTRK-rearranged colorectal cancer: Integration of immunoreactivity 
      pattern, cytogenetic identity, and rearrangement variant.
PG  - 1757-1764
LID - S1590-8658(23)00578-9 [pii]
LID - 10.1016/j.dld.2023.04.019 [doi]
AB  - Fused information from protein status, DNA breakage, and transcripts are still 
      limited because of the low rate of activated-NTRK in colorectal cancer (CRC). In 
      total, 104 archived CRC tissue samples with dMMR were analyzed using 
      immunohistochemistry (IHC), polymerase chain reaction (PCR), and pyrosequencing 
      to mine the NTRK-enriched CRC group, and then subjected to NTRK fusion detection 
      using pan-tyrosine kinase IHC, fluorescence in situ hybridization (FISH), and 
      DNA-/RNA-based next generation sequencing (NGS) assays. Of the 15 NTRK-enriched 
      CRCs, eight NTRK fusions (53.3%, 8/15), including two TPM3(e7)-NTRK1(e10), one 
      TPM3(e5)-NTRK1(e11), one LMNA(e10)-NTRK1(e10), two EML4(e2)-NTRK3(e14), and two 
      ETV6(e5)-NTRK3(e15) fusions, were identified. There was no immunoreactivity for 
      ETV6-NTRK3 fusion. In addition to cytoplasmic staining found in six specimens, 
      membrane positive (TPM3-NTRK1 fusion) and nuclear positive (LMNA-NTRK1 fusion) 
      were also observed in two of them. Atypical FISH-positive types were observed in 
      four cases. Unlike IHC, NTRK-rearranged tumors appeared homogeneous on FISH. 
      ETV6-NTRK3 may be missed in pan-TRK IHC screening for CRC. Regarding break-apart 
      FISH, NTRK detection is difficult because of the diversity of signal patterns. 
      Further research is warranted to identify the characteristics of NTRK-fusion 
      CRCs.
CI  - Copyright (c) 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Wu, Shafei
AU  - Wu S
AD  - Department of Pathology, Peking Union Medical College Hospital, Molecular 
      Pathology Research Center, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan 
      Wangfujing Dongcheng District, Beijing 100730,China.
FAU - Liu, Yuanyuan
AU  - Liu Y
AD  - Department of Pathology, Peking Union Medical College Hospital, Molecular 
      Pathology Research Center, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan 
      Wangfujing Dongcheng District, Beijing 100730,China.
FAU - Shi, Xiaohua
AU  - Shi X
AD  - Department of Pathology, Peking Union Medical College Hospital, Molecular 
      Pathology Research Center, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan 
      Wangfujing Dongcheng District, Beijing 100730,China.
FAU - Zhou, Weixun
AU  - Zhou W
AD  - Department of Pathology, Peking Union Medical College Hospital, Molecular 
      Pathology Research Center, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan 
      Wangfujing Dongcheng District, Beijing 100730,China.
FAU - Zeng, Xuan
AU  - Zeng X
AD  - Department of Pathology, Peking Union Medical College Hospital, Molecular 
      Pathology Research Center, Chinese Academy of Medical Sciences, No.1 Shuaifuyuan 
      Wangfujing Dongcheng District, Beijing 100730,China. Electronic address: 
      zengxuan88@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20230502
PL  - Netherlands
TA  - Dig Liver Dis
JT  - Digestive and liver disease : official journal of the Italian Society of 
      Gastroenterology and the Italian Association for the Study of the Liver
JID - 100958385
RN  - 0 (Biomarkers, Tumor)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Humans
MH  - Biomarkers, Tumor/genetics/analysis
MH  - *Colorectal Neoplasms/genetics
MH  - DNA
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - *Receptor, trkA/genetics/analysis
MH  - Receptor, trkB/genetics
MH  - Receptor, trkC/genetics
OTO - NOTNLM
OT  - Colorectal carcinoma
OT  - Fluorescence in situ hybridization
OT  - Immunohistochemistry
OT  - NTRK fusion
OT  - Next generation sequencing
COIS- Conflicts of interest The other authors declare no conflicts of interest.
EDAT- 2023/05/05 00:42
MHDA- 2023/12/17 09:42
CRDT- 2023/05/04 21:57
PHST- 2023/02/01 00:00 [received]
PHST- 2023/04/13 00:00 [revised]
PHST- 2023/04/17 00:00 [accepted]
PHST- 2023/12/17 09:42 [medline]
PHST- 2023/05/05 00:42 [pubmed]
PHST- 2023/05/04 21:57 [entrez]
AID - S1590-8658(23)00578-9 [pii]
AID - 10.1016/j.dld.2023.04.019 [doi]
PST - ppublish
SO  - Dig Liver Dis. 2023 Dec;55(12):1757-1764. doi: 10.1016/j.dld.2023.04.019. Epub 
      2023 May 2.