PMID- 37145170
OWN - NLM
STAT- MEDLINE
DCOM- 20230508
LR  - 20240515
IS  - 1532-2750 (Electronic)
IS  - 1098-612X (Print)
IS  - 1098-612X (Linking)
VI  - 25
IP  - 5
DP  - 2023 May
TI  - Cat alpha-1-acid glycoprotein enzyme-linked immunosorbent assay: performance 
      characteristics and reference intervals.
PG  - 1098612X231162836
LID - 10.1177/1098612X231162836 [doi]
LID - 1098612X231162836
AB  - OBJECTIVES: The aim of this study was to evaluate performance of a 
      feline-specific ELISA for the measurement of alpha-1-acid glycoprotein (AGP) and 
      to establish a reference interval (RI). METHODS: Surplus serum samples with low 
      (~200 microg/ml), medium (~450 microg/ml) and high (~745 and 930 microg/ml) AGP 
      concentrations were used to assess the intra- and inter-assay coefficients of 
      variation (CVs). The quality goal for bioanalytical method validation was a CV of 
      <20%. Linearity was assessed by serial dilution of a sample with a high AGP 
      concentration. Spike recovery was evaluated by mixing samples with low, medium 
      and high AGP concentrations at different ratios. To establish the RI, residual 
      serum samples from 51 healthy adult cats that were presented for health 
      examinations or blood donation between August 2020 and June 2021 were included. 
      RESULTS: The intra-assay CV was 8.5%, 4.3% and 4.0%, and the inter-assay CV was 
      18.8%, 15.5% and 11.5% for serum samples with low, medium and high AGP 
      concentrations, respectively. Excellent linearity (R(2) = 0.98) was demonstrated 
      for AGP concentrations ranging between 251.6 and 954.4 microg/ml. Average recovery 
      was 95.0-99.7%. The right-sided RI for AGP was 328 microg/ml (90% confidence interval 
      300-354). Age had a statistically significant impact (increasing values with 
      older age, P = 0.0026), but sex did not (P = 0.44), on AGP concentrations. 
      CONCLUSIONS AND RELEVANCE: The ELISA was accurate and showed acceptable precision 
      with the modification of dilution used in this study. AGP concentrations in this 
      population appeared to increase with increasing age.
FAU - Thalmeier, Sabine
AU  - Thalmeier S
AUID- ORCID: 0000-0003-1574-6051
AD  - Clinic for Small Animals (Internal Medicine, Clinical Pathology and Clinical 
      Pathophysiology), Justus-Liebig-University Giessen, Giessen, Germany.
FAU - Gussow, Arne
AU  - Gussow A
AD  - Clinic for Small Animals (Internal Medicine, Clinical Pathology and Clinical 
      Pathophysiology), Justus-Liebig-University Giessen, Giessen, Germany.
FAU - Hauser, Mia K
AU  - Hauser MK
AD  - Clinic for Small Animals (Internal Medicine, Clinical Pathology and Clinical 
      Pathophysiology), Justus-Liebig-University Giessen, Giessen, Germany.
FAU - Bauer, Natali
AU  - Bauer N
AD  - Clinic for Small Animals (Internal Medicine, Clinical Pathology and Clinical 
      Pathophysiology), Justus-Liebig-University Giessen, Giessen, Germany.
FAU - Hazuchova, Katarina
AU  - Hazuchova K
AD  - Clinic for Small Animals (Internal Medicine, Clinical Pathology and Clinical 
      Pathophysiology), Justus-Liebig-University Giessen, Giessen, Germany.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Feline Med Surg
JT  - Journal of feline medicine and surgery
JID - 100897329
RN  - 0 (Orosomucoid)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Cats
MH  - Animals
MH  - *Orosomucoid/analysis
MH  - Enzyme-Linked Immunosorbent Assay/veterinary/methods
MH  - Catalase
PMC - PMC10811989
OTO - NOTNLM
OT  - AGP
OT  - Reference interval
OT  - acute-phase protein
OT  - inflammatory biomarker
OT  - precision
COIS- The authors declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/05/05 12:42
MHDA- 2023/05/08 06:41
PMCR- 2023/05/05
CRDT- 2023/05/05 11:05
PHST- 2023/05/08 06:41 [medline]
PHST- 2023/05/05 12:42 [pubmed]
PHST- 2023/05/05 11:05 [entrez]
PHST- 2023/05/05 00:00 [pmc-release]
AID - 10.1177_1098612X231162836 [pii]
AID - 10.1177/1098612X231162836 [doi]
PST - ppublish
SO  - J Feline Med Surg. 2023 May;25(5):1098612X231162836. doi: 
      10.1177/1098612X231162836.