PMID- 37145276
OWN - NLM
STAT- MEDLINE
DCOM- 20230508
LR  - 20240210
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2667
DP  - 2023
TI  - Immunological Analysis of Cryptococcal Meningoencephalitis in a Murine Model.
PG  - 71-86
LID - 10.1007/978-1-0716-3199-7_5 [doi]
AB  - Cryptococcal meningoencephalitis (CM), caused by the fungal pathogen Cryptococcus 
      neoformans species complex, can lead to high mortality or severe neurological 
      sequelae in survivors that are associated with excessive inflammation in the 
      central nervous system (CNS), especially in those who develop immune 
      reconstitution inflammatory syndrome (IRIS) or postinfectious immune response 
      syndrome (PIIRS). While the means to establish a cause-and-effect relationship of 
      a specific pathogenic immune pathway during CM by human studies are limited, 
      mouse models allow dissection of the potential mechanistic links within the CNS 
      immunological network. In particular, these models are useful for separating 
      pathways contributing predominantly to immunopathology from those important for 
      fungal clearance. In this protocol, we described methods to induce a robust, 
      physiologically relevant murine model of C. neoformans CNS infection that 
      reproduces multiple aspects of human cryptococcal disease immunopathology and 
      subsequent detailed immunological analysis. Combined with tools including gene 
      knockout mice, antibody blockade, cell adoptive transfer, as well as high 
      throughput techniques such as single-cell RNA sequencing, studies using this 
      model will provide new insights regarding the cellular and molecular processes 
      that elucidate the pathogenesis of cryptococcal CNS diseases in order to develop 
      more effective therapeutic strategies.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Xu, Jintao
AU  - Xu J
AD  - Department of Veterans Affairs Health System, Research Service, Ann Arbor VA 
      Healthcare System, Ann Arbor, MI, USA.
AD  - Division of Pulmonary and Critical Care Medicine, Department of Internal 
      Medicine, University of Michigan Health System, Ann Arbor, MI, USA.
FAU - Goughenour, Kristie
AU  - Goughenour K
AD  - Department of Veterans Affairs Health System, Research Service, Ann Arbor VA 
      Healthcare System, Ann Arbor, MI, USA.
AD  - Division of Pulmonary and Critical Care Medicine, Department of Internal 
      Medicine, University of Michigan Health System, Ann Arbor, MI, USA.
FAU - Underwood, W Rex
AU  - Underwood WR
AD  - Department of Veterans Affairs Health System, Research Service, Ann Arbor VA 
      Healthcare System, Ann Arbor, MI, USA.
AD  - Division of Pulmonary and Critical Care Medicine, Department of Internal 
      Medicine, University of Michigan Health System, Ann Arbor, MI, USA.
FAU - Olszewski, Michal A
AU  - Olszewski MA
AD  - Department of Veterans Affairs Health System, Research Service, Ann Arbor VA 
      Healthcare System, Ann Arbor, MI, USA. olszewsm@umich.edu.
AD  - Division of Pulmonary and Critical Care Medicine, Department of Internal 
      Medicine, University of Michigan Health System, Ann Arbor, MI, USA. 
      olszewsm@umich.edu.
LA  - eng
GR  - I01 BX000656/BX/BLRD VA/United States
GR  - IK6 BX003615/BX/BLRD VA/United States
GR  - IK6 BX005966/BX/BLRD VA/United States
GR  - T32 HL007749/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - *Cryptococcosis
MH  - Disease Models, Animal
MH  - *Cryptococcus neoformans
MH  - *Meningoencephalitis/microbiology/pathology
MH  - Central Nervous System/pathology
PMC - PMC10588511
MID - NIHMS1935639
OTO - NOTNLM
OT  - Cryptococcal meningoencephalitis
OT  - Mice model
OT  - Neuroinflammation
EDAT- 2023/05/05 12:42
MHDA- 2023/05/08 06:41
PMCR- 2023/10/20
CRDT- 2023/05/05 11:10
PHST- 2023/05/08 06:41 [medline]
PHST- 2023/05/05 12:42 [pubmed]
PHST- 2023/05/05 11:10 [entrez]
PHST- 2023/10/20 00:00 [pmc-release]
AID - 10.1007/978-1-0716-3199-7_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2667:71-86. doi: 10.1007/978-1-0716-3199-7_5.