PMID- 37145284
OWN - NLM
STAT- MEDLINE
DCOM- 20230531
LR  - 20230531
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2667
DP  - 2023
TI  - Genetic Mouse Models of Pneumocystis Pneumonia.
PG  - 169-179
LID - 10.1007/978-1-0716-3199-7_13 [doi]
AB  - Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major 
      challenge in drug susceptibility testing and in understanding host/pathogen 
      interactions is that Pneumocystis spp. are not viable in vitro. Continuous 
      culture of the organism is not currently available, and therefore, developing new 
      drug targets is very limited. Due to this limitation, mouse models of 
      Pneumocystis pneumonia have proven to be an invaluable resource to researchers. 
      In this chapter, we provide an overview of selected methods used in mouse models 
      of infection including, in vivo Pneumocystis murina propagation, routes of 
      transmission, genetic mouse models available, a P. murina life form-specific 
      model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), 
      and the experimental parameters associated with these models.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Hoving, J Claire
AU  - Hoving JC
AD  - AFRICA CMM Medical Mycology Research Unit, Institute of Infectious Disease and 
      Molecular Medicine (IDM), Cape Town, South Africa. Jennifer.hoving@uct.ac.za.
AD  - Department of Pathology, Faculty of Health Sciences, University of Cape Town, 
      Cape Town, South Africa. Jennifer.hoving@uct.ac.za.
AD  - MRC Centre for Medical Mycology, University of Exeter, Exeter, UK. 
      Jennifer.hoving@uct.ac.za.
FAU - Munyonho, Ferris T
AU  - Munyonho FT
AD  - Centre for Translational Research in Infection and Inflammation, Tulane School of 
      Medicine, New Orleans, LA, USA.
FAU - Kolls, Jay K
AU  - Kolls JK
AD  - Centre for Translational Research in Infection and Inflammation, Tulane School of 
      Medicine, New Orleans, LA, USA. jkolls1@tulane.edu.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - *Mycobacterium tuberculosis
MH  - *Pneumocystis carinii/genetics
MH  - *Pneumonia, Pneumocystis/genetics
OTO - NOTNLM
OT  - Immune response
OT  - Immunocompromised
OT  - Lung fungal infection
OT  - Pneumocystis murina
OT  - Rag1-deficient
EDAT- 2023/05/05 12:42
MHDA- 2023/05/08 06:41
CRDT- 2023/05/05 11:10
PHST- 2023/05/08 06:41 [medline]
PHST- 2023/05/05 12:42 [pubmed]
PHST- 2023/05/05 11:10 [entrez]
AID - 10.1007/978-1-0716-3199-7_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2667:169-179. doi: 10.1007/978-1-0716-3199-7_13.