PMID- 37146711
OWN - NLM
STAT- MEDLINE
DCOM- 20230526
LR  - 20230526
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 176
DP  - 2023 Jun
TI  - Comprehensive analysis and identification of the circ_0084615/miR-451a/MEF2D axis 
      in benzo(a)pyrene exposed tumor cells in hepato-carcinogenesis.
PG  - 113810
LID - S0278-6915(23)00212-0 [pii]
LID - 10.1016/j.fct.2023.113810 [doi]
AB  - Hepatocellular carcinoma (HCC) is caused by genetic and epigenetic alterations, 
      as well as abnormal lifestyle and dietary habits, including contaminated food 
      intake. Benzo(a)pyrene (B[a]P), derived from deep-fried meats, is regarded as the 
      main dietary factor for tumorigenesis in epidemiological investigations. Although 
      various studies have illustrated the adverse effects of B[a]P in malignancy 
      through cell and animal models, the correlation between B[a]P exposure and 
      clinical data remain to be explored. In the present study, we analyzed and 
      identified novel B[a]P-associated circular RNA (circRNA) from microarray 
      databases of liver tumor cells and HCC patient samples. Considering that circRNA 
      regulates mRNA as a miRNA sponge, molecular circRNA-miRNA-mRNA interactions based 
      on the stimulation of B[a]P exposure were predicted and established. Furthermore, 
      up-regulated circ_0084615 in B[a]P-treated tumor cells was verified as a miRNA 
      sponge via fluorescence in situ hybridization (FISH) assays, and the repression 
      between circ_0084615 and target miR-451a exhibited a contrasting effect on 
      hepatocarcinogenesis. Therefore, we performed integrated bioinformatics analysis 
      and molecular experiments to establish the circ_0084615/miR-451a/MEF2D pathway, 
      which provided a better understanding of the adverse effects of fried food 
      preference on human health.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Mu, Wei
AU  - Mu W
AD  - School of Public Health, Shanghai Jiao Tong University School of Medicine, 
      Shanghai, 200025, China.
FAU - Gu, Pengfei
AU  - Gu P
AD  - School of Public Health, Shanghai Jiao Tong University School of Medicine, 
      Shanghai, 200025, China.
FAU - Song, Weiwei
AU  - Song W
AD  - School of Public Health, Shanghai Jiao Tong University School of Medicine, 
      Shanghai, 200025, China.
FAU - Zhu, Tengfei
AU  - Zhu T
AD  - School of Public Health, Shanghai Jiao Tong University School of Medicine, 
      Shanghai, 200025, China.
FAU - Wang, Wenbo
AU  - Wang W
AD  - Department of Oncology, Shanghai Tenths People's Hospital, School of Medicine, 
      Tongji University, Shanghai, 200072, China.
FAU - Zhou, Yanfeng
AU  - Zhou Y
AD  - School of Public Health, Shanghai Jiao Tong University School of Medicine, 
      Shanghai, 200025, China. Electronic address: yfzhou@shsmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230503
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (RNA, Circular)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (MEF2D protein, human)
RN  - 0 (MEF2 Transcription Factors)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Carcinoma, Hepatocellular/genetics/pathology
MH  - RNA, Circular/genetics
MH  - Benzo(a)pyrene/toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - *Liver Neoplasms/genetics/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - *MicroRNAs/genetics/metabolism
MH  - Carcinogenesis/genetics
MH  - RNA, Messenger
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - MEF2 Transcription Factors/genetics/metabolism
OTO - NOTNLM
OT  - Benzo(a)pyrene
OT  - Circular RNA
OT  - Competing endogenous RNA
OT  - Hepatocellular carcinoma
OT  - Prognostic biomarker
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/05/06 09:42
MHDA- 2023/05/26 06:42
CRDT- 2023/05/05 19:25
PHST- 2022/06/08 00:00 [received]
PHST- 2023/04/27 00:00 [revised]
PHST- 2023/05/02 00:00 [accepted]
PHST- 2023/05/26 06:42 [medline]
PHST- 2023/05/06 09:42 [pubmed]
PHST- 2023/05/05 19:25 [entrez]
AID - S0278-6915(23)00212-0 [pii]
AID - 10.1016/j.fct.2023.113810 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2023 Jun;176:113810. doi: 10.1016/j.fct.2023.113810. Epub 2023 
      May 3.