PMID- 37154326
OWN - NLM
STAT- MEDLINE
DCOM- 20230510
LR  - 20231213
IS  - 1872-2075 (Electronic)
IS  - 1000-3061 (Linking)
VI  - 39
IP  - 4
DP  - 2023 Apr 25
TI  - [Lamin B1 regulates the growth of hepatocellular carcinoma cells by influencing 
      telomerase activity].
PG  - 1609-1620
LID - 10.13345/j.cjb.220625 [doi]
AB  - Lamin B1 (LMNB1) is highly expressed in liver cancer tissues, and its influence 
      and mechanism on the proliferation of hepatocellular carcinoma cells were 
      explored by knocking down the expression of the protein. In liver cancer cells, 
      siRNAs were used to knock down LMNB1. Knockdown effects were detected by Western 
      blotting. Changes in telomerase activity were detected by telomeric repeat 
      amplification protocol assay (TRAP) experiments. Telomere length changes were 
      detected by quantitative real-time polymerase chain reaction (qPCR). CCK8, 
      cloning formation, transwell and wound healing were performed to detect changes 
      in its growth, invasion and migration capabilities. The lentiviral system was 
      used to construct HepG2 cells that steadily knocked down LMNB1. Then the changes 
      of telomere length and telomerase activity were detected, and the cell aging 
      status was detected by SA-beta-gal senescence staining. The effects of tumorigenesis 
      were detected by nude mouse subcutaneous tumorigenesis experiments, subsequent 
      histification staining of tumors, SA-beta-gal senescence staining, fluorescence in 
      situ hybridization (FISH) for telomere analysis and other experiments. Finally, 
      the method of biogenesis analysis was used to find the expression of LMNB1 in 
      clinical liver cancer tissues, and its relationship with clinical stages and 
      patient survival. Knockdown of LMNB1 in HepG2 and Hep3B cells significantly 
      reduced telomerase activity, cell proliferation, migration and invasion 
      abilities. Experiments in cells and tumor formation in nude mice had demonstrated 
      that stable knockdown of LMNB1 reduced telomerase activity, shortened telomere 
      length, senesced cells, reduced cell tumorigenicity and KI-67 expression. 
      Bioinformatics analysis showed that LMNB1 was highly expressed in liver cancer 
      tissues and correlated with tumor stage and patient survival. In conclusion, 
      LMNB1 is overexpressed in liver cancer cells, and it is expected to become an 
      indicator for evaluating the clinical prognosis of liver cancer patients and a 
      target for precise treatment.
FAU - Wang, Ruiguan
AU  - Wang R
AD  - Faculty of Hepato-Pancreato-Biliary Surgery, Chinese People's Liberation Army 
      General Hospital, Beijing 100853, China.
AD  - Department of Hepatobiliary Surgery, the Eighth Medical Center, Chinese People's 
      Liberation Army General Hospital, Beijing 100091, China.
FAU - Chen, Si
AU  - Chen S
AD  - Department of Obstetrics and Gynecology, Hunan Prevention and Treatment Institute 
      for Occupational Diseases, Changsha 410021, Hunan, China.
FAU - Sun, Zhijia
AU  - Sun Z
AD  - Radiotherapy Department, People's Liberation Army Air Force Characteristic 
      Medical Center, Beijing 100142, China.
FAU - Wang, Shikun
AU  - Wang S
AD  - Physician of Information and Communication Brigade of Xinjiang Military 
      Prefecture, Ngari Prefecture 859499, Tibet, China.
FAU - Wang, Jie
AU  - Wang J
AD  - School of Medicine, Shihezi University, Shihezi 832000, Xinjiang, China.
FAU - Qin, Lingmei
AU  - Qin L
AD  - Institute of Biotechnology, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100089, China.
FAU - Li, Jiangbo
AU  - Li J
AD  - Institute of Biotechnology, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100089, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Telomerase/genetics/metabolism
MH  - *Carcinoma, Hepatocellular/genetics
MH  - *Liver Neoplasms/genetics
MH  - Telomere Shortening
MH  - In Situ Hybridization, Fluorescence
MH  - Mice, Nude
MH  - Telomere/metabolism/pathology
MH  - Carcinogenesis
MH  - Lamin Type B
OTO - NOTNLM
OT  - cell senescence
OT  - hepatocellular carcinoma
OT  - nuclear lamina protein B1 (LMNB1)
OT  - telomerase
OT  - telomere
EDAT- 2023/05/08 13:41
MHDA- 2023/05/10 06:42
CRDT- 2023/05/08 07:13
PHST- 2023/05/10 06:42 [medline]
PHST- 2023/05/08 13:41 [pubmed]
PHST- 2023/05/08 07:13 [entrez]
AID - 10.13345/j.cjb.220625 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2023 Apr 25;39(4):1609-1620. doi: 
      10.13345/j.cjb.220625.