PMID- 37155009
OWN - NLM
STAT- MEDLINE
DCOM- 20230526
LR  - 20240131
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 6
DP  - 2023 Jun
TI  - MiR-125 family improves the radiosensitivity of head and neck squamous cell 
      carcinoma.
PG  - 5307-5317
LID - 10.1007/s11033-023-08364-x [doi]
AB  - BACKGROUND: MiRNAs can affect the radiosensitization of head and neck squamous 
      cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family 
      members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect 
      on radiation in laryngeal squamous cell cancer (LSCC). METHODS: First, we 
      systematically analyzed the role of the miR-125 family in HNSCC using the TCGA 
      database and found that miR-125a-5p is associated with radiotherapy. We then 
      performed comprehensive enrichment analysis of miR-125a-5p and predicted target 
      genes. Then, we performed transfection, cell proliferation assays, reverse 
      transcription polymerase chain reaction, apoptosis assays, micronucleus tests, 
      and western blotting on hep-2 cells selected with puromycin. RESULTS: MiR-125 
      family members exhibited significantly different expression in HNSCC. They were 
      significantly associated with tumor-node-metastasis staging, clinical stages, and 
      histological grades. Radiation therapy had a statistically effect on miR-125 
      family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall 
      survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of 
      miR-125a-5p. The proliferation rate of cells transfected with lentivirus vector 
      expressing miR-125a-5p was significantly reduced compared to the other groups. 
      The radiation effect was enhanced in cells transfected with miR-125a-5p. The 
      ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly 
      higher than that of the Ad-control group. Western blotting analysis revealed that 
      miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p 
      may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes. 
      CONCLUSIONS: MiR-125 family members could be prognostic biomarkers of HNSCC and 
      improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating 
      miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the 
      effect of radiotherapy on LSCC.
CI  - (c) 2023. The Author(s).
FAU - Wang, Qi-Wei
AU  - Wang QW
AD  - Department of Otolaryngology, Head and Neck Surgery, Harbin Medical University, 
      Harbin, People's Republic of China.
FAU - Sun, Ya-Nan
AU  - Sun YN
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, No.246, Xuefu Road, Harbin, 150081, 
      People's Republic of China. syn2767@126.com.
FAU - Tan, Li-Jun
AU  - Tan LJ
AD  - Department of Oncology, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, People's Republic of China.
FAU - Zhao, Jian-Nan
AU  - Zhao JN
AD  - Department of Otolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, 
      People's Republic of China.
FAU - Zhou, Xiao-Jie
AU  - Zhou XJ
AD  - Department of Otolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, 
      People's Republic of China.
FAU - Yu, Tian-Jiao
AU  - Yu TJ
AD  - Department of Otolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, 
      People's Republic of China.
FAU - Liu, Jiang-Tao
AU  - Liu JT
AD  - Department of Otolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital of Harbin Medical University, No.23, Youzheng Street, Harbin, 150001, 
      People's Republic of China. ljt89103@163.com.
LA  - eng
GR  - No. 81772874/National Natural Science Foundation of China/
GR  - 81272965/National Natural Science Foundation of China/
GR  - H2017033/Natural Science Foundation of Heilongjiang Province/
GR  - 2018019/Health Commission of Heilongjiang Province/
GR  - No.2021M12/the Scientific Research Innovation Fund of the First Affiliated 
      Hospital of Harbin Medical University/
PT  - Journal Article
PT  - Retracted Publication
DEP - 20230508
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN125 microRNA, human)
SB  - IM
RIN - Mol Biol Rep. 2024 Jan 30;51(1):239. PMID: 38289564
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/radiotherapy
MH  - Tumor Suppressor Protein p53/genetics
MH  - Cell Line, Tumor
MH  - *MicroRNAs/genetics
MH  - Radiation Tolerance/genetics
MH  - *Head and Neck Neoplasms/genetics/radiotherapy
MH  - Gene Expression Regulation, Neoplastic
MH  - Cell Proliferation/genetics
PMC - PMC10209316
OTO - NOTNLM
OT  - Cancer Genome Atlas
OT  - Head and neck squamous cell carcinoma prognosis
OT  - Laryngeal squamous cell cancer
OT  - Radiotherapy
COIS- The authors have no conflicts of interest to declare.
EDAT- 2023/05/08 13:42
MHDA- 2023/05/26 06:42
PMCR- 2023/05/08
CRDT- 2023/05/08 11:19
PHST- 2022/11/30 00:00 [received]
PHST- 2023/02/25 00:00 [accepted]
PHST- 2023/05/26 06:42 [medline]
PHST- 2023/05/08 13:42 [pubmed]
PHST- 2023/05/08 11:19 [entrez]
PHST- 2023/05/08 00:00 [pmc-release]
AID - 10.1007/s11033-023-08364-x [pii]
AID - 8364 [pii]
AID - 10.1007/s11033-023-08364-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 Jun;50(6):5307-5317. doi: 10.1007/s11033-023-08364-x. Epub 
      2023 May 8.