PMID- 37157183
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231024
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 101
IP  - 7
DP  - 2023 Aug
TI  - The potential of dietary nanoparticles to enhance allergenicity of milk proteins: 
      an in vitro investigation.
PG  - 625-638
LID - 10.1111/imcb.12649 [doi]
AB  - In recent years, the popularity of dietary nanoparticles (NPs) in the food 
      industry as additives has raised concerns because of the lack of knowledge about 
      potential adverse health outcomes ensuing from the interactions of NPs with 
      components of the food matrix and gastrointestinal system. In this study, we used 
      a transwell culture system that consisted of human colorectal adenocarcinoma 
      (Caco-2) cells in the apical insert and Laboratory of Allergic Diseases 2 mast 
      cells in the basal compartment to study the effect of NPs on milk allergen 
      delivery across the epithelial layer, mast cell responses and signaling between 
      epithelial and mast cells in allergenic inflammation. A library of dietary 
      particles (silicon dioxide NPs, titanium dioxide NPs and silver NPs) that varied 
      in particle size, surface chemistry and crystal structures with or without 
      pre-exposure to milk was used in this investigation. Milk-interacted particles 
      were found to acquire surface corona and increased the bioavailability of milk 
      allergens (casein and beta-lactoglobulin) across the intestinal epithelial layer. 
      The signaling between epithelial cells and mast cells resulted in significant 
      changes in the early phase and late-phase activation of the mast cells. This 
      study suggested that antigen challenge in mast cells with the presence of dietary 
      NPs may cause the transition of allergic responses from an immunoglobulin 
      E (IgE)-dependent mechanism to a mixed mechanism (both IgE-dependent and 
      IgE-independent mechanisms).
CI  - (c) 2023 the Australian and New Zealand Society for Immunology, Inc.
FAU - Xu, Ke
AU  - Xu K
AUID- ORCID: 0000-0002-0471-0523
AD  - Department of Food Science and Agricultural Chemistry, McGill University, 
      Sainte-Anne-de-Bellevue, QC, Canada.
FAU - Phue, Wut Hmone
AU  - Phue WH
AD  - Department of Food Science and Agricultural Chemistry, McGill University, 
      Sainte-Anne-de-Bellevue, QC, Canada.
FAU - Basu, Niladri
AU  - Basu N
AD  - Department of Natural Resource Sciences, McGill University, 
      Sainte-Anne-de-Bellevue, QC, Canada.
FAU - George, Saji
AU  - George S
AD  - Department of Food Science and Agricultural Chemistry, McGill University, 
      Sainte-Anne-de-Bellevue, QC, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230508
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Milk Proteins)
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - Caco-2 Cells
MH  - *Milk Proteins
MH  - Allergens
MH  - *Nanoparticles/chemistry
MH  - Immunoglobulin E
OTO - NOTNLM
OT  - Cytokines
OT  - dietary nanoparticles
OT  - food-grade silicon dioxide
OT  - immune response
OT  - silver
OT  - titanium dioxide
EDAT- 2023/05/09 06:42
MHDA- 2023/10/23 12:42
CRDT- 2023/05/09 01:03
PHST- 2023/02/17 00:00 [revised]
PHST- 2023/04/06 00:00 [revised]
PHST- 2022/12/21 00:00 [received]
PHST- 2023/04/11 00:00 [accepted]
PHST- 2023/10/23 12:42 [medline]
PHST- 2023/05/09 06:42 [pubmed]
PHST- 2023/05/09 01:03 [entrez]
AID - 10.1111/imcb.12649 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2023 Aug;101(7):625-638. doi: 10.1111/imcb.12649. Epub 2023 
      May 8.