PMID- 3715810
OWN - NLM
STAT- MEDLINE
DCOM- 19860703
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 42
IP  - 4
DP  - 1986 May 15
TI  - Comparison of the effects of low molecular weight heparin and unfractionated 
      heparin on activation of human platelets in vitro.
PG  - 435-47
AB  - An unfractionated heparin (UFH) and a depolymerised derivative of low molecular 
      weight heparin (LMWH) have been compared for their ability to activate platelets 
      suspended in citrated plasma (PRP) or after washing and suspension in hepes 
      buffered tyrode containing fibrinogen. Neither heparin alone induced aggregation 
      of washed platelets, but UFH and to a much lesser extent LMWH, induced 
      aggregation of platelets in PRP. Both heparins caused significant enhancement of 
      a low concentration of ADP-induced activation of PRP and, again, the effect of 
      LMWH was somewhat less than that of UFH. UFH produced a marked potentiation of 
      ADP-induced activation of washed platelets and LMWH was about a third as potent. 
      In addition, UFH induced a potentiation of PAF-induced aggregation and 
      dense-granule release in PRP, a property not shared by LMWH. In PRP, UFH was 
      three times more potent at inhibiting thrombin-induced aggregation and 
      dense-granule release, as might be expected from their specific activities in the 
      KCCT and thrombin time assay. However, with washed platelets, both heparins were 
      equivalent at inhibiting thrombin-induced aggregation, dense-granule release and 
      elevation of cytosolic free calcium ([Ca++]i) as monitored by quin 2 
      fluorescence. UFH and LMWH alone did not induce a change in [Ca++]i, nor had they 
      any effect on ADP- or PAF-induced elevation of [Ca++]i.
FAU - Westwick, J
AU  - Westwick J
FAU - Scully, M F
AU  - Scully MF
FAU - Poll, C
AU  - Poll C
FAU - Kakkar, V V
AU  - Kakkar VV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Platelet Activating Factor)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.5 (Thrombin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology/physiology
MH  - Calcium/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Heparin/*pharmacology/physiology
MH  - Humans
MH  - Male
MH  - Molecular Weight
MH  - Platelet Activating Factor/pharmacology/physiology
MH  - Platelet Aggregation/*drug effects
MH  - Thrombin/pharmacology/physiology
EDAT- 1986/05/15 00:00
MHDA- 1986/05/15 00:01
CRDT- 1986/05/15 00:00
PHST- 1986/05/15 00:00 [pubmed]
PHST- 1986/05/15 00:01 [medline]
PHST- 1986/05/15 00:00 [entrez]
AID - 10.1016/0049-3848(86)90207-0 [doi]
PST - ppublish
SO  - Thromb Res. 1986 May 15;42(4):435-47. doi: 10.1016/0049-3848(86)90207-0.