PMID- 37160747
OWN - NLM
STAT- MEDLINE
DCOM- 20230511
LR  - 20230511
IS  - 1539-6304 (Electronic)
IS  - 1088-5412 (Linking)
VI  - 44
IP  - 3
DP  - 2023 May 1
TI  - Diagnostic utility of basophil CD203c expression in beta-lactam allergy.
PG  - 200-207
LID - 10.2500/aap.2023.44.230013 [doi]
AB  - Background: A diagnosis of immunoglobulin E (IgE) mediated reactions to beta-lactam 
      (BL) antibiotics is still challenging because of the limited availability of 
      skin-prick test (SPT), and standardization issues, particularly with newer BLs, 
      are still ongoing. Because encouraging data are increasingly emerging in the use 
      of basophil activation tests in the diagnosis of IgE-mediated drug 
      hypersensitivity reactions, in this study, we aimed to determine CD203c 
      expression, a basophil surface marker, in the diagnosis of IgE-mediated 
      hypersensitivity to BL antibiotics. Methods: This study included two groups of 
      subjects. The first group (group 1) (n = 20) included patients with a diagnosis 
      of IgE-mediated allergy to BLs as confirmed through STs or drug provocation 
      tests, and the control group consisted of healthy volunteers (group 2) (n = 24). 
      Expression of CD203c by flow cytometry was studied in samples stimulated by two 
      different concentrations of six different BL antibiotics. A stimulation index >/= 2 
      was considered a positive response. Results: The study groups had comparable age 
      and sex distribution. In the entire group, the sensitivity and specificity of 
      CD203c were 29.4% (5 out of 17) and 82.6% (19 out of 23), respectively. When 
      considering the single reactors, two among four patients who were allergic to 
      amoxicillin demonstrated upregulation of CD203c with amoxicillin, which makes 50% 
      sensitivity. The specificity was 100%. Conclusion: Our data demonstrated that 
      assessment of CD203c in the diagnosis of IgE-mediated reactions to BLs provided 
      encouraging results, particularly with amoxicillin allergy. However, this finding 
      needs to be verified in a larger number of cases.
FAU - Celik, Gulfem Elif
AU  - Celik GE
AD  - From the, Department of Chest Diseases, Division of Immunology and Allergy, 
      Ankara, Turkey, and.
FAU - Guloglu Karatas, Deniz
AU  - Guloglu Karatas D
AD  - Department of Pediatric Immunology and Allergy, Ankara, Turkey.
FAU - Aydin, Omur
AU  - Aydin O
AD  - From the, Department of Chest Diseases, Division of Immunology and Allergy, 
      Ankara, Turkey, and.
FAU - Dogu, Figen
AU  - Dogu F
AD  - Department of Pediatric Immunology and Allergy, Ankara, Turkey.
FAU - Ikinciogullari, Aydan
AU  - Ikinciogullari A
AD  - Department of Pediatric Immunology and Allergy, Ankara, Turkey.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Allergy Asthma Proc
JT  - Allergy and asthma proceedings
JID - 9603640
RN  - 0 (beta-Lactams)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Monobactams)
RN  - 804826J2HU (Amoxicillin)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Humans
MH  - Basophils
MH  - beta-Lactams/adverse effects
MH  - *Hypersensitivity
MH  - Immunoglobulin E
MH  - *Drug Hypersensitivity/diagnosis
MH  - Monobactams
MH  - Amoxicillin
MH  - Anti-Bacterial Agents/adverse effects
EDAT- 2023/05/10 06:42
MHDA- 2023/05/11 06:42
CRDT- 2023/05/10 00:53
PHST- 2023/05/11 06:42 [medline]
PHST- 2023/05/10 06:42 [pubmed]
PHST- 2023/05/10 00:53 [entrez]
AID - 10.2500/aap.2023.44.230013 [doi]
PST - ppublish
SO  - Allergy Asthma Proc. 2023 May 1;44(3):200-207. doi: 10.2500/aap.2023.44.230013.